我们以链霉菌为模式系统，聚焦于微生物次级代谢产物生物合成途径的解析及其复杂分子调控机制的阐明，为定向改良菌株的生产能力、设计组合生物合成途径、激活隐性基因簇进而获得新型代谢产物和药物提供重要的理论指导；同时，针对具有重要应用价值的天然产物，包括具有复杂结构的糖类化合物等，进行结构-活性评价研究，并通过合成生物学方法重构其合成体系，实现重要药物分子的高效生物制造。

1.Li J*, Zhang J, Tan H*. Microbial production of chondroitin sulfate and its derivatives. Sci China Life Sci. 2025 Mar;68(3):871-873. doi: 10.1007/s11427-024-2805-1. Epub 2024 Dec 10. PMID: 39666218.

2. Chen H, Liu X, Wei B, Tian Y, Li Y, Zhang J, Tan H, Li J*. Identification of a novel butenolide signal system to regulate high production of tylosin in Streptomyces fradiae. Appl Microbiol Biotechnol. 2025 Jan 22;109(1):18. doi: 10.1007/s00253-024-13396-9. PMID: 39841199; PMCID: PMC11754346.

3. Li J, Wang K, Luo S, Tian Y, Li Y, Hu S, Tan H, Zhang J*, Li J*. Co-expression of a pair of interdependent regulators coding genes ovmZ and ovmW awakens the production of angucyclinones antibiotics in Streptomyces neyagawaensis. Microb Cell Fact. 2024 Jul 18;23(1):202. doi: 10.1186/s12934-024-02478-y. PMID: 39026365; PMCID: PMC11264864.

4. Liu X, Li Y, Li J, Ren J, Li D, Zhang S, Wu Y, Li J*, Tan H*, Zhang J*. Cinnamoyl lipids as novel signaling molecules modulate the physiological metabolism of cross-phylum microorganisms. Commun Biol. 2024 Oct 1;7(1):1231. doi: 10.1038/s42003-024-06950-8. PMID: 39354171; PMCID: PMC11445547.

5. Li Y, Guan H, Li J, Zhang J, Wang Y, Li J, Tan H. An intricate regulation of WblA controlling production of silent tylosin analogues and abolishment of expressible nikkomycin. Sci China Life Sci. 2023 Mar;66(3):612-625. doi: 10.1007/s11427-022-2199-1. Epub 2023 Jan 4. PMID: 36607495.

6. Tian Y, Li D, Wang K, Wei B, Zhang J, Li J*. An efficient method for targeted cloning of large DNA fragments from Streptomyces. Appl Microbiol Biotechnol. 2023 Sep;107(18):5749-5760. doi: 10.1007/s00253-023-12685-z. Epub 2023 Jul 24. PMID: 37486353.

7. Liu X, Li J, Li Y, Li J, Sun H, Zheng J, Zhang J, Tan H. A visualization reporter system for characterizing antibiotic biosynthetic gene clusters expression with high-sensitivity. Commun Biol. 2022 Sep 2;5(1):901. doi: 10.1038/s42003-022-03832-9. PMID: 36056143; PMCID: PMC9440138.

8. Francisco AB, Li J, Farghli AR, Kanke M, Shui B, Munn PR, Grenier JK, Soloway PD, Wang Z, Reid LM, Liu J, Sethupathy P. Chemical, Molecular, and Single-nucleus Analysis Reveal Chondroitin Sulfate Proteoglycan Aberrancy in Fibrolamellar Carcinoma. Cancer Res Commun. 2022 Jul 18;2(7):663-678. doi: 10.1158/2767-9764.CRC-21-0177. PMID: 36923282; PMCID: PMC10010304.

9. Li J, Su G, Xu Y, Arnold K, Pagadala V, Wang C, Liu J. Synthesis of 3-O-Sulfated Heparan Sulfate Oligosaccharides Using 3-O-Sulfotransferase Isoform 4. ACS Chem Biol. 2021 Oct 15;16(10):2026-2035. doi: 10.1021/acschembio.1c00474. Epub 2021 Aug 5. PMID: 34351732; PMCID: PMC8526403.

10. Li J, Sparkenbaugh EM, Su G, Zhang F, Xu Y, Xia K, He P, Baytas S, Pechauer S, Padmanabhan A, Linhardt RJ, Pawlinski R, Liu J. Enzymatic Synthesis of Chondroitin Sulfate E to Attenuate Bacteria Lipopolysaccharide-Induced Organ Damage. ACS Cent Sci. 2020 Jul 22;6(7):1199-1207. doi: 10.1021/acscentsci.0c00712. Epub 2020 Jul 1. PMID: 32724854; PMCID: PMC7379384.

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