Microfluidics and Analytical Microbiology Applications
09/2007 - 08/2011 Postdoctoral Fellow, University of Chicago, (with Professor Rustem F. Ismagilov)
09/2011 - 11/2013 Principle Investigator, Department of Chemistry, Renmin University
11/2013 - Professor, Institute of Microbiology, Chinese Academy of Sciences
09/2007 - 08/2011 Professor, Savaid Medical School, University of the Chinese Academy of Sciences
09/2007 - 08/2011 Doctoral supervisor, Biomedical Sciences College & Shandong Medical Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences
代表性图片
Low-cost digital nucleic acids amplification for precision diagnostics (Xu, Anal Chem, 2016; Hu & Xu, Anal Chem. 2017)
High-throughput single-cell functional screening by fluorescence activated droplet sorting (Qiao, Lab Chip 2018)
High-throughput single cell cultivation from microbial communities (Jiang & Dong, AEM, 2016)
近期代表性论文(* 通讯作者)
Publications:
Google Scholar Page
Dulab @ ResearchGate
2022
89. Ye, S.#; Li, C.M.#; Zheng, X.*; Huang, W.H.; Tao Y.; Yu Y.H.; Yang, L.M.; Lan, Y.; Ma, L.*; Bian, S.T.*; Du, W.B.* OsciDrop: A Versatile Deterministic Droplet Generator, Analytical Chemistry, 2022, 94, 6, 2918-2925.
88. Qiao, Y.X.; Hu, R.; Chen, D.W.; Wang, L.; Wang, Z.Y.; Yu, H.Y.; Fu, Y.; Li, C.L.; Dong, Z.Y.; Weng, Y.-X.*; Du W.B.* Fluorescence-Activated Droplet Sorting of PET Degrading Microorganisms, Journal of Hazardous Materials, 2022, 424, 127417.
2021
89. Hu, B.Y.; Ye, S.; Chen, D.W.; Xie, B.L.; Hu, R.; Qiao, Y.X.; Yu, Y.H.; Yu, H.Y.; Zheng, X.; Lan, Y.; Du, W.B.* Tunable and Contamination-free Injection with Microfluidics by Stepinjection, Analytical Chemistry, 2021, doi:10.1021/acs.analchem.1c02721.
88. Yun, J.L.; Crombie, A.T.; Ul Haque, F.M; Cai, Y.F.; Zheng, X.W.; Wang, J.; Jia, Z.J.; Murrell, J.C.; Wang, Y.F.*; Du, W.B.* Revealing the community and metabolic potential of active methanotrophs by targeted metagenomics in the Zoige wetland of the Tibetan Plateau, Environmental Microbiology, 2021, 23(11), 6520-6535.
87. Xie, B.L.; Wang, J.; Nie, Y.; Chen, D.W.; Hu, B.Y.; Wu, X.L.; Du, W.B.* EpicPCR-Directed Cultivation of a Candidatus Saccharibacteria Symbiont Reveals a Type IV Pili-dependent Epibiotic Lifestyle, bioRxiv, 2021, doi: 10.1101/2021.07.08.451036.
86. Ye, S.; Zheng, X.*; Li, C.M.; Huang, W.H.; Tao Y.; Yu Y.H.; Yang, L.M.; Lan, Y.; Ma, L.; Bian, S.T.*; Du, W.B.* OsciDrop: A versatile on-demand droplet generator, bioRxiv, 2021, doi: 10.1101/2021.06.14.448273.
85. Chen, D.W.; Nie, M.Y.; Tang, W.; Zhang, Y.W.; Qiao, Y.X.; Yun, J.L.; Wang, J.; Lan, Y.; Ma, L.; Chen, Y.H.*; Du, W.B.* Microfluidic Streptomyces Cultivation for Whole Lifecycle Characterization and Phenotypic Assays Enabled by Nanogap-stabilized Air-Water Interface, bioRxiv, 2021, doi: 10.1101/2021.05.13.443940.
84. Qiao, Y.X.; Hu, R.; Chen, D.W.; Wang, L.; Wang, Z.Y.; Yu, H.Y.; Fu, Y.; Li, C.L.; Dong, Z.Y.; Weng, Y.-X.*; Du W.B.* Fluorescence-Activated Droplet Sorting of PET Degrading Microorganisms, Journal of Hazardous Materials, 2021, 424, 127417.
83. Yun, J.L.; Du, W.B.* Two Metagenome-Assembled Genomes of Hydrogen-Dependent Methanomassiliicoccales Methanogens from the Zoige Wetland of the Tibetan Plateau, Microbiology Resource Announcements, 2021, 10(17), e00021-21.
82. Hu, B.Y.; Xu, P.; Ma, L.; Chen, D.W.; Wang, J.; Dai, X.; Huang, L.; Du, W.B.* One cell at a time: droplet-based microbial cultivation, screening, and sequencing, Marine Life Science & Technology, 2021, 3, 169-188.
81. Cui, Y.L.; Chen, Y.C.; Liu, X.Y.; Dong, S.J.; Tian, Y.E.; Qiao, Y.X.; Mitra, R.; Han, J.; Li, C.L.; Han, X.; Liu, W.D.; Chen, Q.; Wei, W.Q.; Wang, X.; Du, W.B.; Tang, S.Y.; Xiang, H.; Liu, H.Y.;Liang, Y.; Houk, K.; Wu, B.* Computational redesign of a PETase for plastic biodegradation under ambient condition by the GRAPE strategy, ACS Catalysis, 2021, 11, 1340-1350.
80. 张婧琳; 胡然; 靳增军; 杜文斌*; 梁宇红*. 基于微流控的生物被膜研究进展及口腔微生态系统应用展望, 微生物学报, 2021, 61(5), 1091-1105.
79. 张思琦; 王剑; 兰英; 贠娟莉; 陈建; 何湘伟*; 杜文斌*. 抗菌活性放线菌的界面微滴移液互作分离筛选方法, 微生物学通报, 2021, 48(1), 325-335.
78. Zheng, X.W.; Liu, W.; Dai, X.; Zhu, Y.X.; Wang, J.F.; Zhu, Y.Q.; Zheng, H.J.; Huang, Y.; Dong, Z.Y.; Du, W.B.; Zhao, F.Q.; Huang, L.* Extraordinary diversity of viruses in deep-sea sediments as revealed by metagenomics without prior virion separation, Environmental Microbiology, 2021, 23(2):728-743.
2020
77. 段云峰,王升跃,陈禹保,杨瑞馥,李后开,朱怀球,童贻刚,杜文斌,付钰,胡松年,王军,辛玉华,赵方庆,鲍一明,张雯,曾明,牛海涛,周欣,李岩,崔生辉,袁静,李俊桦,王加义,刘东来,倪铭,孙青,邓晔,朱宝利.* 微生物组测序与分析专家共识, 生物工程学报, 2020, 36(12), 2516-2524.
76. Ruan, C.J.; Wang, J.; Zheng, X.W.; Song, L.; Zhu, Y.X.; Huang, Y.; Lu, Z.J.; Du, W.B.; Huang, L.; Dai, X.* Halovulum marinum sp. nov., isolated from deep seawater of the Northwest Indian Ocean, and emended description of the genus Halovulum, International Journal of Systematic and Evolutionary Microbiology, 2020,70(8):4523-4530.
75. Wang, J.; Ruan, C.-J.; Song, L.; Zhu, Y.-X.; Li, A.; Zheng, X.-W.; Wang, L.; Lu, Z.-J.; Huang, Y.; Du, W.B.; Zhou, Y.G.; Huang, L.; Dai, X.* Gimesia benthica sp. nov., a novel planctomycete isolated from a deep-sea water sample of the Northwest Indian Ocean, International Journal of Systematic and Evolutionary Microbiology, 2020, 70(7), 4384-4389.
74. Tao, Y.; Yun, J.L.; Wang, J.; Xu, P.; Li, C.M.; Liu, H.T.; Lan, Y.*; Pan, J.Z.*; Du, W.B.* High-Performance Detection of Mycobacterium bovis in Milk Using Digital LAMP, Food Chemistry, 2020, 327, 126945.
73. Liu, Y.Y.; Xu, J.J.; Tao, Y.; Fang, T.; Du, W.B.; Ye, A.P.* Rapid and accurate identification of marine microbes with single-cell Raman spectroscopy, Analyst, 2020, 145, 3297-3305.
72. Qu, H.J.; Yu, M.C.; Du, W.B.; Xu, L.; Lv, W.Y.; Shen, F.* Slip molding for precision fabrication of microparts, Langmiur, 2020, 36, 2, 585-590.
71. Hu, B.Y.; Xu, B.X.; Yun, J.L.*; Wang, J.; Xie, B.L.; Li, C.M.; Yu, Y.H.; Lan, Y.; Zhu, Y.X.; Dai, X.; Huang, Y.; Huang, L.; Pan, J.Z.*; Du, W.B.* High-throughput Single-cell Cultivation Reveals Underexplored Rare Biosphere in Deep-sea Sediments Along the Southwest Indian Ridge, Lab On a Chip, 2020, 20, 363-372.
70. Chen, D.W.; Yun, J.L.; Qiao, Y.X.; Hu, R.; Hu, B.Y.; Wang, J.; Du, W.B. 2020. "Microfluidic techniques for high-throughput cell analysis." In Biomimetic Microengineering, edited by H. J. Kim, CRC Taylor & Francis, pp.165-189.
69. Yun, J.L.#; Zheng, X.W.#; Xu. P.#; Zheng, X.; Xu, J.Y.; Cao, C.; Fu, Y.S.; Xu, B.X.; Dai, X.; Wang, Y.; Liu, H.T.; Yi, Q.L.; Zhu, Y.X.; Wang, J.; Wang, L.; Dong, Z.Y.; Huang, L.*; Huang, Y.Y.*; Du, W.B.* Interfacial Nanoinjection-based Nanoliter Single-cell Analysis, Small, 2020, 16, 1903739. (Invited Full Paper)
68. Liu, Y.P.; Feng, H.C.; Fu, R.X.; Zhang, N.; Du, W.B.; Shen, Q.R.; Zhang, R.F.* Induced root-secreted D-galactose functions as a chemoattractant and enhances the biofilm formation of Bacillus velezensis SQR9 in an McpA-dependent manner, Applied microbial and cell physiology, 2020, 104, 785-797.
2019
67. Wei, W.P.#; Meng, C.#; Wang, Y.H.; Huang, Y.S.; Du, W.B.; Li, H.F.; Liu, Y.F.; Song, H.; Tang, F.S.* The interaction between self - assembling peptides and emodin and the controlled release of emodin from in-situ hydrogel, Artificial Cells, Nanomedicine, and Biotechnology, 2019, 47, 3961-3975.
66. Zhukov, D.V.; Khorosheva, E.M.; Khazaei, T.; Du, W.B.; Selck, D.A.; Shishkin, A.; Ismagilov, R.F.* Microfluidic SlipChip device for multistep multiplexed biochemistry on a nanoliter scale, Lab on a Chip, 2019, 47, 3961-3975.
65. Lyu, W.Y.; Yu, M.C.; Qu, H.J.; Yu, Z.Q.; Du, W.B.; Shen, F.* Slip-driven microfluidic devices for nucleic acid analysis, Biomicrofluidics, 2019, 13, 041502.
64. Yi, Q.L.#; Cai, D.Y.#; Xiao, M.; Nie, M.Y.; Cui, Q.N.; Cheng, J.W.; Li, C.M.; Feng, J.; Urban, G.; Xu, Y.-C.*; Lan, Y.*; Du, W.B.* Direct Antimicrobial Susceptibility Testing of Bloodstream Infection on SlipChip, Biosensors & Bioelectronics, 2019, 135, 200-207.
63. Nie, M.Y.; Zheng, M.; Li, C.M.; Shen, F.; Liu, M.H.; Luo, H.B.; Song, X.H.; Lan, Y.*; Pan, J.Z.*; Du, W.B.* Assembled Step Emulsification Device for Multiplex Droplet Digital PCR, Analytical Chemistry, 2019, 91, 1779–1784.
62. Chen, D.W.; Liu, S.-J.; Du, W.B.* Chemotactic screening of imidazolinone-degrading bacteria by microfluidic SlipChip, Journal of Hazardous Materials, 2019, 366, 512-519.
61. Feng, H.C.; Zhang, N.; Fu, R.X.; Liu, Y.P.; Krell, T.; Du, W.B.; Shao, J.H.; Shen, Q.R.; Zhang, R.F.* Recognition of dominant attractants by key chemoreceptors mediates recruitment of plant growth-promoting rhizobacteria, Environmental Microbiology, 2019, 21, 402-415.
2018
60. Ruan, C.J.; Zheng, X.-W.; Wang, J.; Song, L.; Zhu, Y.-X.; Du, W.B.; Lu, Z.-J.; Huang, Y.; Huang, L.*; Dai, X.* Hyphobacterium indicum sp. nov., isolated from deep seawater, and emended description of the genus Hyphobacterium, International Journal of Systematic and Evolutionary Microbiology, 2018, 68(12), 3760-3765.
59. Zhai, Z.M.; Nie, M.Y.; Guan, Y.; Zhang, F.Q.; Chen, L.; Muhammad; Du, W.B.; Liu, G.; Tian, Y.C.; Huang, Q.* A microfluidic surface-enhanced Raman spectroscopy approach for assessing the particle number effect of AgNPs on cytotoxicity, Ecotoxicology and Environmental Safety, 2018, 162, 529-535.
58. Liao, S.L.; Tao, Y.; Du, W.B.*; Wang, Y.P.* Interfacial emulsification: an emerging monodisperse droplet generation method for microreactors and bioanalysis, Langmuir, 2018, 34, 11655-11666. (Invited Feature Article)
57. 何渊慧; 奕巧莲; 杜文斌; 郑波*. 抗菌药物敏感性快速检测方法研究进展, 中国感染控制杂志, 2018, 17, 455-460.
56. Zhou, N.; Sun, Y.T.; Chen, D.W.; Du, W.B.; Yang, H.; Liu, S.J.* Harnessing microfluidic streak plate technique to investigate the gut microbiome of Reticulitermes chinensis, MicrobiologyOpen, 2018, e654.
55. Feng H.C.; Zhang, N.; Du, W.B.; Zhang, H.H.; Liu, Y.P.; Fu, R.X.; Shao, J.H.; Zhang, G.S.; Shen, Q.R.; Zhang, R.F.* Identification of chemotaxis compounds in root exudates and their sensing chemoreceptors in plant growth-promoting rhizobacteria Bacillus amyloliquefaciens SQR9, Molecular Plant-Microbe Interactions, 2018, 31, 995-1005.
54. Xu, B.X.; Hu, B.Y.; Wang, J.; Lan, Y.; Zhu, Y.X.; Dai, X.; Huang, L.; Huang, Y.*; Du, W.B.* Virgibacillus indicus sp. nov. and Virgibacillus profundi sp. nov, two moderately halophilic bacteria isolated from marine sediment by using the microfluidic streak plates, International Journal of Systematic and Evolutionary Microbiology, 2018, 68, 2015-2023.
53. Jin, Z.J.; Nie, M.Y.; Zhao, T.H.; Xu, J.Y.; Chen, D.W.; Yun, J.L.; Hu, R.; Ma, L.Z.; Du, W.B.* Dynamic Sessile-droplet Habitats for Controllable Cultivation of Bacterial Biofilm, Small, 2018, 14, 1800658.
52. Wang, S.Y.; Wang, X.Y.; Wang, L.; Pu, Q.S.; Du, W.B.; Guo, G.S.* Plasma assisted alignment in the fabrication of microchannel-array-based in-tube solid phase microextraction microchips packed with TiO2 nanoparticles for phosphopeptide analysis, Analytica Chimica Acta, 2018, 1018, 70-77.
51. Cai, D.Y.; Yi, Q.L.; Shen, C.H.; Lan, Y.; Urban, G.; Du, W.B.* Direct enrichment of pathogens from physiological samples of high conductivity and viscosity using H-filter and positive dielectrophoresis, Biomicrofluidics, 2018, 12, 014109.
50. Qiao, Y.X.; Zhao, X.Y.; Zhu, J.; Tu, R.; Dong, L.B.; Wang, L.; Dong, Z.Y.; Wang, Q.H.; Du, W.B.* Fluorescence-Activated Droplet Sorting of Lipolytic Microorganisms Using a Compact Optical System, Lab On a Chip, 2018, 18, 190-196.
2017
49. Liao, S.L; Tao, X.L.; Ju, Y.J.; Feng, J.*; Du, W.B.*; Wang, Y.P.* Multi-Channel Dynamic Interfacial Printing: An Alternative Multi-Component Droplet Generation Technique for Lab in a Drop, ACS Applied Materials and Interfaces, 2017, 9, 4345-4352.
48. 贾仲君*, 蔡元锋, 贠娟莉, 杜文斌. 单细胞、显微计数和高通量测序典型水稻土微生物组的技术比较, 微生物学报, 2017, 57(6), 899-919.
47. Hu, Y.#; Xu, P.#; Luo, J.; He, H.X.*; Du, W.B.* Absolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplification, Analytical Chemistry, 2017, 89 (1), 745-750. (#Equal contribution).
2016
46.Dong, L.B.; Chen, D.W.; Liu, S.J.; Du, W.B.* Automated Chemotactic Sorting and Single-cell Cultivation of Microbes using Droplet Microfluidics, Scientific Reports, 2016, 6, 24192.
45.Liao, S.L.; He, Y.L.; Wang, D.G.; Dong, L.B.; Du, W.B.*; Wang, Y.P.* Dynamic Interfacial Printing for Monodisperse Droplets and Polymeric Microparticles, Advanced Materials Technologies, 2016, 1, 1600021.
44.Xu, P.; Zheng, X.; Tao, Y.; Du, W.B.* Cross-Interface Emulsification for Generating Size-Tuneable Droplets, Analytical Chemistry, 2016, 88, 3171-3177.
43.Jiang, C.Y.#; Dong, L.B.#; Zhao, J.K.#; Hu, X.F.; Shen, C.H.; Qiao, Y.X.; Zhang, X.Y.; Wang, Y.P.; Ismagilov, R.F.; Liu, S.J.*; Du, W.B.* High throughput Single-cell Cultivation on Microfluidic Streak Plates, Applied Environmental Microbiology, 2016, 82, 2210-2218. (#Equal contribution, Spotlight)
2015
42.Yu, S.; Su, T.T.; Wu, H.J.; Liu, S.H.; Wang, D.; Zhao, T.H.; Jin, Z.J.; Du, W.B.; Zhu, M.J.; Chua, S.L.; Yang, L.; Zhu, D.Y.; Gu, L.C.*; Ma, L.Z.* PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix, Cell Research, 2015, 25, 1352-1367.
41.Jia, H.Y.*; He, Y.L.; Zhang, X.Y.; Du, W.B.; Wang, Y.P.* Integrating Ultra-Thermal-Sensitive Fluids into Elastomers for Multifunctional Flexible Sensors, Advanced Electronic Materials, 2015, 1, 1500029.
40.Huang, X.P.; Yang, Y.D.; Shi, J.Z.; Ngo, H.T.; Shen, C.H.; Du, W.B.; Wang, Y.P.* High-Internal-Phase Emulsion Tailoring Polymer Amphiphilicity towards an Efficient NIR-Sensitive Bacteria Filter,Small, 2015, 11, 4876-4883.
39.Wu, Z.Q.; Du, W.B.; Li, J.Y.; Xia, X.H.*; Fang, Q.* Establishment of a finite element model for extracting chemical reaction kinetics in a micro-flow injection system with high throughput sampling, Talanta, 2015, 140, 176-182.
38.Liu, X.; Yi, Q.L.; Han, Y.Z.; Liang, Z.N.; Shen, C.H.; Zhou, Z.Y.; Sun J.L.; Li, Y.Z.; Du, W.B.*; Cao, R.* A Robust Microfluidic Device for Synthesis and Crystal Growth of Organometallic Polymers with Highly Organized Structures, Angewandte Chemie International Edition, 2015, 54, 1846-1850.
2014
37.Cai, D.Y.; Xiao, M.; Xu, P.; Xu, Y.C.*; Du, W.B.* An Integrated Microfluidic Device Utilizing Dielectrophoresis and Multiplex Array PCR for Point-of-Care Detection of Pathogens, Lab On a Chip, 2014, 14, 3917-3924. (HOT Article, Front Cover)
36. Shen, C.H.; Xu, P.; Huang, Z.; Liu, S.J.*; Du, W.B.* Bacterial Chemotaxis on SlipChip, Lab On a Chip, 2014, 14, 3074-3080.
35. Wang, S.J.; Chen, S.M.; Wang, J.N.; Xu, P.; Luo, Y.M.; Nie, Z.X.*; Du, W. B.* Interface Solution Isoelectric Focusing with in situ MALDI-TOF Mass Spectrometry, Electrophoresis, 2014, 35, 2528-2533.
34. 袁会领; 董立兵; 涂然*; 杜文斌; 贾士儒; 王钦宏* 液滴微流控芯片系统中微液滴特性表征及氨基酸检测应用, 生物工程学报, 2014, 30, 139-146.
2013
33.Huang, X.P.; Qian, Q.P.; Zhang, X.Y.; Du, W.B.; Xu, H.P.; Wang, Y.P.* Assembly of Carbon Nanotubes on Polymer Particles: Towards Rapid Shape Change by Near-Infrared Light, Particle & Particle Systems Characterization, 2013, 30, 235-240.
32. Wang, J.; Zhang, X.Y.; Huang, X.P.; Wang, S.J.; Qian, Q.P.; Du, W.B.; Wang, Y.P.* Forced Assembly of Water-Dispersible Carbon Nanotubes Trapped in Paper for Cheap Gas Sensors, Small, 2013, 9, 3759-3764.
Previous Publications:
31.Shen, F.; Sun, B.; Kreutz, J.E.; Davydova, E.K.; Du, W.B.; Reddy, P.L.; Joseph, L.J.; Ismagilov, R.F.* Multiplexed Quantification of Nucleic Acids with Large Dynamic Range Using Multivolume Digital RT-PCR on a Rotational SlipChip Tested with HIV and Hepatitis C Viral Load, Journal of the American Chemical Society, 2011, 133, 17705-17712.
30.Pompano, R.R.; Liu, W.S.; Du, W.B.; Ismagilov, R.F.* Microfluidics Using Spatially Defined Arrays of Droplets in One, Two, and Three Dimensions, Annual Review of Analytical Chemistry, 2011, 4, 59-81.
29.Kim, H.J.; Du, W.B.; Ismagilov, R.F.* Complex Function by Design Using Spatially Pre-structured Synthetic Microbial Communities: Degradation of Pentachlorophenol in the Presence of Hg(II), Integrative Biology, 2011, 3, 126-133.
28.Shen, F.; Davydova, E.K.; Du, W.B.; Kreutz, J.E.; Piepenburg, O.; Ismagilov, R.F.* Digital Isothermal Quantification of Nucleic Acids via Simultaneous Chemical Initiation of Recombinase Polymerase Amplification Reactions on SlipChip, Analytical Chemistry, 2011, 83, 3533-3540.
27.Kreutz, J.E.; Munson, T.; Huynh, T.; Shen, F.; Du, W.B.; Ismagilov, R.F.* Theoretical Design and Analysis of Multivolume Digital Assays with Wide Dynamic Range Validated Experimentally with Microfluidic Digital PCR, Analytical Chemistry, 2011, 83, 8158-8168.
26.Gu, S.Q.; Zhang, Y.X.; Zhu, Y.; Du, W.B.; Yao, B.; Fang, Q.* Multifunctional Picoliter Droplet Manipulation Platform and Its Application in Single Cell Analysis, Analytical Chemistry, 2011, 83, 7570-7576.
25.Du, W.B.#; Sun, M.#; Gu, S.Q.#; Zhu, Y.; Fang, Q.* Automated Microfluidic Screening Assay Platform Based on DropLab, Analytical Chemistry, 2010, 82, 9941-9947 (#Equal contribution).
24.Li, L.; Du, W.B.*; Ismagilov, R.F.* Multiparameter Screening on SlipChip Used for Nanoliter Protein Crystallization Combining Free Interface Diffusion and Microbatch Methods, Journal of the American Chemical Society, 2010, 132, 112-119.
23.Li, L.; Du, W.B.; Ismagilov, R.F.* User-Loaded SlipChip for Equipment-Free Multiplexed Nanoliter-Scale Experiments, Journal of the American Chemical Society, 2010, 132, 106-111.
22.Kreutz, J.E.; Shukhaev, A.; Du, W.B.; Druskin, S.; Daugulis, O.; Ismagilov, R.F.* Evolution of Catalysts Directed by Genetic Algorithms in a Plug-Based Microfluidic Device Tested with Oxidation of Methane by Oxygen, Journal of the American Chemical Society, 2010, 132, 3128-3132.
21.Shen, F.; Du, W.B.; Davydova, E.K.; Karymov, M.A.; Pandey, J.; Ismagilov, R.F.* Nanoliter Multiplex PCR Arrays on a SlipChip, Analytical Chemistry, 2010, 82, 4606-4612.
20.Shen, F.; Du, W.B.; Kreutz, J.E.; Fok, A.; Ismagilov, R.F.* Digital PCR on a SlipChip, Lab on a Chip, 2010, 10, 2666-2672.
19.Liu, W.S.; Chen, D.L.; Du, W.B.; Nichols, K.P.; Ismagilov, R.F.* SlipChip for Immunoassays in Nanoliter Volumes, Analytical Chemistry, 2010, 82, 3276-3282.
18.Du, W.B.; Li, L.; Nichols, K.P.; Ismagilov, R.F.* SlipChip, Lab on a Chip, 2009, 9, 2286-2292.
17.Liu, W.S.; Kim, H.J.; Lucchetta, E.M.; Du, W.B.; Ismagilov, R.F.* Isolation, Incubation, and Parallel Functional Testing and Identification by FISH of Rare Microbial Single-copy Cells from Multi-species Mixtures Using the Combination of Chemistrode and Stochastic Confinement, Lab on a Chip, 2009, 9, 2153-2162.
16.Zhang, T.; Fang, Q.*; Du, W.B.; Fu, J.L. Microfluidic Picoliter-Scale Translational Spontaneous Sample Introduction for High-Speed Capillary Electrophoresis, Analytical Chemistry, 2009, 81, 3693-3698.
15.Chen, D.L.; Du, W.B.; Ismagilov, R.F.* Using TIRF Microscopy to Quantify and Confirm Efficient Mass Transfer at the Substrate Surface of the Chemistrode, New Journal of Physics, 2009, 11, 75017.
14.姚波; 何巧红; 杜文斌; 石晓彤; 方群*. 微流控高通量试样引入技术的研究进展, 色谱, 2009, 27, 662-666.
13.Chen, D.L.#; Du, W.B.#; Liu, Y.#; Liu, W.S.#; Kuznetsov, A.; Mendez, F.E.; Philipson, L.H.; Ismagilov, R.F.* The chemistrode: A Droplet-based Microfluidic Device for Stimulation and Recording with High Temporal, Spatial, and Chemical Resolution, Proceedings of the National Academy of Sciences of the United States of America, 2008, 105, 16843-16848 (#Equal contribution).
12.Huang, Y.Z.; Du, W.B.; Pan, J.Z.; Fang, Q.* Microfluidic chip-based valveless flow injection analysis system with gravity-driven flows, Analyst, 2008, 133, 1237-1241.
11.Fang, Q.*; Shi, X.T.; Du, W.B.; He, Q.H.; Shen, H.; Fang, Z.L. High-throughput microfluidic sample-introduction systems, Trac-Trends in Analytical Chemistry, 2008, 27, 521-532.
10.Fan, X.F.; Li, Q.; Wang, S.L.; Xu, Z.R.*; Du, W.B.; Fang, Q.; Fang, Z.L. High-throughput analysis of DNA fragments using a miniaturized CE system combined with a slotted-vial array sample introduction system, Electrophoresis, 2008, 29, 4733-4738.
9.叶晓兰; 杜文斌; 古淑青; 潘建章; 牟颖; 方群*. 小型微流控芯片流式细胞仪的研制, 分析化学, 2008, 36, 1443-1446.
8.He, Q.H.; Fang, Q.*; Du, W.B.; Fang, Z.L. Fabrication of a monolithic sampling probe system for automated and continuous sample introduction in microchip-based CE, Electrophoresis, 2007, 28, 2912-2919.
7.Du, W.B.; Fang, Q.*; Fang, Z.L. Microfluidic Sequential Injection Analysis in a Short Capillary, Analytical Chemistry, 2006, 78, 6404-6410.
6.Sun, M.; Du, W.B.; Fang, Q.* Microfluidic Liquid-liquid Extraction System based on Stopped-flow Technique and Liquid Core Waveguide Capillary, Talanta, 2006, 70, 392-396.
5.Du, W.B.; Fang, Q.*; He, Q.H.; Fang, Z.L. High-throughput Nanoliter Sample Introduction Microfluidic Chip-based Flow Injection Analysis System with Gravity-driven Flows, Analytical Chemistry, 2005, 77, 1330-1337.
4.He, Q.H.; Fang, Q.*; Du, W.B.; Huang, Y.Z.; Fang, Z.L.* An automated electrokinetic continuous sample introduction system for microfluidic chip-based capillary electrophoresis, Analyst, 2005, 130, 1052-1058.
3.刘军; 方群*; 杜文斌. 缺口型试样管阵列进样系统与紫外检测毛细管电泳联用技术, 分析化学, 2005, 33, 1799-1802.
2.方群*; 杜文斌; 何巧红; 方肇伦. 微流控芯片高通量试样引入系统的研究, 高等学校化学学报, 2004, 25, 1442-1446.
1.杜文斌; 方群*; 方肇伦. 基于液芯波导原理的微流控芯片长光程光度检测系统, 高等学校化学学报, 2004, 25, 610-613.
申请或授权专利
申请中国发明专利、中国实用新型专利、PCT及美国专利40余项,转化8项。
授权中国发明专利13项,实用新型3项,美国专利9项
研究涉及微生物学、合成生物学、微流控学、生物工程、仪器工程、医学检验等多学科交叉。研究利用微流控液滴技术实现高通量的微生物单细胞分离和筛选:包括开发自动化的装置和芯片,实现大规模的单细胞分离、培养、互作和测序,致力于环境不可培养微生物资源培养、稀有物种和极端微生物获取、潜在产酶和产活性代谢物功能菌株筛选和评价、复杂微生物群落生态功能和进化研究等。此外,我们将针对临床精准诊断和生物安全现场快速检测等开展应用技术研发,推动微流控技术的产业化应用。
近期开展的课题包括:
1.微生物单细胞分离、培养和高通量分选:利用液滴微流控技术平台在单细胞水平对环境和富集培养样品中的功能菌进行高通量分选、培养和分析,加快微生物资源的收集挖掘和评估。
2.不可培养微生物单细胞测序:结合流式细胞分选(FACS)和纳升体积单细胞基因组扩增技术,实现环境中未培养功能微生物菌群的单细胞基因组测序和功能分析。
3.数字PCR技术方法开发和应用:自主研制基于振动微滴发生技术(OsciDrop) 的一体化、自动化数字PCR平台,用于临床微生物(细菌,真菌,病毒等)快速高灵敏多重多靶点检测。
4.病原微生物分离、鉴定和耐药分析:基于微流控的临床样品病原微生物分离和分子鉴定,以及病原菌的药物敏感性定量分析。
National Natural Science Foundation of China
National Natural Science Foundation of China
国家自然科学基金青年基金项目,32100073,基于荧光纳米传感材料和微流控超高通量筛选的塑料微生物降解研究,2022/01-2024/12,主持(乔雨歆)
国家自然科学基金委员会重大研究计划“水圈微生物驱动地球元素循环的机制”,91951103,海洋未培养甲基营养菌群驱动C1循环的微流控组学分析,2020/01-2022/12,主持(杜文斌)
国家自然科学基金优秀青年科学基金项目, 21822408, 微流控微生物组分析,2019/01-2021/12,主持(杜文斌)
国家自然科学基金面上基金项目,31970091,畜禽角蛋白废弃物资源化利用关键酶类的超高通量筛选挖掘技术研究,2020/01-2023/12,主持(陈栋炜)
国家自然科学基金面上基金项目,41977196,高通量单细胞技术及多组学结合的若尔盖湿地低温甲烷氧化菌代谢机制研究,2020/01-2023/12,主持(贠娟莉)
Ministry of Science and Technology of the People's Republic of China
National Key Research and Development Program of China (2021YFC2301000), 国家重点研发计划 “病原学与防疫技术体系研究”, 难培养和微量病原体靶向培养技术研究, 2021/12-2024/11,主持(杜文斌)
National Key Research and Development Program of China (2021YFA0717000), 国家重点研发计划 “变革性技术关键科学问题”专项, 高通量培养筛选鉴定健康相关微生物的关键技术, 2021/12-2026/11,参加(钱韦主持)
National Key Research and Development Program of China (2021YFC2103300), 国家重点研发计划 “绿色生物制造”专项, 工业菌种高通量选育技术与装备研发及应用, 2021/12-2024/11,参加(王钦宏主持)
National Key Research and Development Program of China (2018YFC0310703), 国家重点研发计划 “深海关键技术与装备专项”, 深海热液区病毒多样性及其与宿主互作机制研究, 2018/08-2021/12,参加(黄力主持)
Chinese Academy of Sciences
Key Program of Frontier Sciences of the Chinese Academy of Sciences (QYZDB-SSW-SMC008), 中国科学院前沿科学重点研究项目, 新型微尺度单细胞菌株选育体系与应用, 2016/08-2020/12, 主持(杜文斌)
China Ocean Mineral Resources R&D Association
International ocean resource survey and exploration (DY135-B-02), 国际海域资源调查与开发“十三五”, 深海稀有微生物多样性及其菌种与基因资源获取和评价,2016/01-2020/09,参加(杜文斌)
Center for Ocean Mega-Science, Chinese Academy of Sciences
Senior User Project of RV KEXUE (KEXUE2019GZ05), “科学”号高端用户项目,深海特殊微生物资源挖掘与技术集成,2019/09-2021/12,参加(向华主持)
Previous Projects (已结题项目)
Novozymes A/S, 2017-2020 (杜文斌)
National Natural Science Foundation of China (31470221), 国家自然科学基金面上项目,阵列微流控技术在血感染病原微生物快速培养、分离和鉴定中的应用,2015/01-2018/12,主持(杜文斌)
National Natural Science Foundation of China (31500081), 国家自然科学基金青年项目,基于趋化微流控芯片的除草剂降解菌的高通量筛选与降解特性的研究,2016/01-2018/12,主持(陈栋炜)
National Natural Science Foundation of China (31500447), 国家自然科学基金青年项目,若尔盖湿地退化过程中甲烷氧化菌菌群动态演替及其生长机理研究,2016/01-2018/12,主持(贠娟莉)
National Natural Science Foundation of China (31230003), 国家自然科学基金重点项目,丛毛单胞菌等对芳环类污染物的趋化、降解及其在生物修复中的作用,2013/01-2017/12,参加(杜文斌)
National Natural Science Foundation of China (21205134), 国家自然科学基金青年项目,用于细菌趋化性定量检测和筛选的液滴微流控平台及其应用,2013/01-2015/12,主持(杜文斌)
Ministry of Science and Technology of the People's Republic of China (2016YFC0100900), 国家重点研发计划 “数字诊疗装备研发”,新一代高通量数字PCR关键技术及应用研究, 2016/07-2018/12,主持(杜文斌)
Ministry of Science and Technology of the People's Republic of China (2016YFE0205800), 国家重点研发计划 “战略性国际科技创新合作重点专项”, 人、畜传染性疾病预防监控技术, 2017/09-2020/12(李学兵主持)
Ministry of Science and Technology of the People's Republic of China (2012AA092103), 国家863计划项目,深海未培养微生物单细胞基因组分析技术,2012/01-2015/12,参与(杜文斌) Beijing Municipal Science & Technology Commission (Z161100000116042), 北京市科技计划"生命科学领域前沿技术",用于细菌耐药快速检测的微流控芯片的研制, 2016/01-2018/12, 主持(杜文斌)
Chinese Academy of Sciences (KFZD-SW-219-4), 中国科学院重点部署项目, 人体与环境健康的微生物组共性技术研究项目,课题四:微生物组功能解析技术与计算方法学,2017/10-2019/09,子课题(杜文斌)
Chinese Academy of Sciences (XDB15040102), 中国科学院B类战略性先导科技专项“土壤-微生物调控机制研究”, 项目四:土壤微生物研究的新技术与方法,2015/01-2018/12,子课题(杜文斌)
Chinese Academy of Sciences (KFJ-SW-STS-165), 中国科学院科技服务网络计划,生物高通量检测分析服务网络: 液滴微流控检测分选平台及应用, 2016/01-2017/12, 参与(杜文斌)
Program of China Ocean Mineral Resources R&D Association (DY125-15-R-02), 国际海域资源调查与开发“十二五”重大项目,深海典型环境微生物的群落特征与应用潜力发掘: 微流控技术在深海环境微生物分离培养中的应用,2011/01-2015/12,子课题(杜文斌)