1.高价值化合物的生物合成

　　 用合成生物学手段在微生物细胞中对高价值化合物（包括药物及中间体、食品添加剂及其他化合物）的生物合成途径进行重构、设计和改造，利用细胞工厂来大量合成这些化合物。通过设计针对不同化合物的特异性识别工具，对化合物的胞内合成产量进行高通量筛选。该研究方向旨在将自然界大量存在的廉价资源用微生物高效转化为自然界不存在或含量很少的一些高价值化合物。我们主要采用合成生物学手段、定向进化方法等对化合物的生物合成途径以及生产宿主菌进行设计或改造，以提高化合物的生物合成效率。侧重于芳香族化合物如羟基酪醇、香兰素、姜黄素等产品的生物合成研究。

1. Yufeng Cao#, La Xiang#, Jasmina Nikodinovic-Runic, Veselin Maslak, Jian-Ming Jin*, Chaoning Liang*, Shuang-Yan Tang*. Improving enzymatic degradation of high-crystallinity poly(ethylene terephthalate). Chinese Journal of Catalysis 2025, 71: 375-389.
2. Zhen zhen#, La Xiang#, Shizhong Li#, Hongji Li, Yanyan Lei, Wei Chen, Jian-Ming Jin*, Chaoing Liang*, Shuang-Yan Tang*. Designing a whole-cell biosensor applicable for S-adenosyl-L-methionine-dependent methyltransferases. Biosensors and Bioelectronics 2025, 268: 116904.
3. La Xiang#, Xuanxuan Zhang#, Yanyan Lei#, Jieyuan Wu#, Guangru Yan, Wei Chen, Shizhong Li, Wenzhao Wang, Jian-Ming Jin*, Chaoning Liang*, Shuang-Yan Tang*. Designing a highly efficient type III polyketide whole-cell catalyst with minimized byproduct formation. Biotechnology for Biofuels and Bioproducts 2024, 17: 93.
4. Xiaochong Zhu#, Jieyuan Wu#, Shizhong Li#, La Xiang, Jian-Ming Jin*, Chaoning Liang*, Shuang-Yan Tang*. An artificial biosynthetic pathway for efficient synthesis of vanillin, a feruloyl-CoA-derived natural product from eugenol. Journal of Agricultural and Food Chemistry 2024, 72: 6463-6470.
5. Jieyuan Wu#, Chaoning Liang#,*, Yufei Li, Yueting Zeng, Xu Sun, Peixia Jiang, Wei Chen, Dandan Xiong, Jian-Ming Jin*, Shuang-Yan Tang*. Engineering and application of LacI mutants with stringent expressions. Microbial Biotechnology 2024, 17: e14427.
6. Xuanxuan Zhang#, Yufeng Cao#, Ying Liu, Yanyan Lei, Ruixue Zhai, Wei Chen, Guizhi Shi*, Jian-Ming Jin*, Chaoning Liang*, Shuang-Yan Tang*. Designing glucose utilization "highway" for recombinant biosynthesis. Metabolic Engineering 2023, 78: 235-247.
7. Yanyan Lei, Wei Chen, La Xiang, Jieyuan Wu, Zhen Zhen, Jian-Ming Jin*, Chaoning Liang*, Shuang-Yan Tang*. Engineering an SspB-mediated degron for novel controllable protein degradation. Metabolic Engineering 2022, 74: 150-159.
8. Xuanxuan Zhang#, Yang He#, Zhe Wu, Guoxia Liu, Yong Tao, Jian-Ming Jin*, Wei Chen*, Shuang-Yan Tang*. Whole-cell biosensors aid exploration of vanillin transmembrane transport. Journal of Agricultural and Food Chemistry 2021, 69(10): 3114-3123.
9. Shizhong Li#, Chaoning Liang#, Guoxia Liu, Jian-Ming Jin*, Yong Tao*, Shuang-Yan Tang*. De novo biosynthesis of chlorogenic acid using an artificial microbial community. Journal of Agricultural and Food Chemistry 2021, 69(9): 2816-2825.
10. Yinglu Cui, Yanchun Chen, Xinyue Liu, Saijun Dong, Yu’e Tian, Yuxin Qiao, Ruchira Mitra, Jing Han, Chunli Li, Xu Han, Weidong Liu, Quan Chen, Wangqing Wei, Xin Wang, Wenbin Du, Shuangyan Tang, Hua Xiang, Haiyan Liu, Yong Liang, Kendall N. Houk, Bian Wu*. Computational redesign of a PETase for plastic biodegradation under ambient condition by the GRAPE strategy. ACS Catalysis 2021, 11: 1340-1350. doi.org/10.1021/acscatal.0c05126
11. Jieyuan Wu, Wei Chen, Yutong Zhang, Xuanxuan Zhang, Jian-Ming Jin*, Shuang-Yan Tang*. Metabolic engineering for improved curcumin biosynthesis in Escherichia coli. Journal of Agricultural and Food Chemistry 2020, 68(39):10772-10779
12. Xiangyu Ji, Huiwei Zhao, Hua Zhu, Kun Zhu*, Shuang-Yan Tang*, Chunbo Lou*. CRISPRi/dCpf1-mediated dynamic metabolic switch to enhance butenoic acid production in Escherichia coli. Applied Microbiology and Biotechnology 2020, 104: 5385-5393.
13. Jun Yao, Yang He, Nannan Su, Sakshibeedu R. Bharath, Yong Tao, Jian-Ming Jin*, Wei Chen*, Haiwei Song*, Shuang-Yan Tang*. Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps. Nature Communications 2020, 11: 1515.
14. Jun Yao#, Jing Li#, Dandan Xiong, Yuanyuan Qiu, Guizhi Shi, Jian-Ming Jin*, Yong Tao*, Shuang-Yan Tang*. Development of a highly efficient and specific L-theanine synthase. Applied Microbiology and Biotechnology 2020, 104: 3417-3431.
15. Chaoning Liang, Xuanxuan Zhang, Jieyuan Wu, Shanshan Mu, Zhe Wu, Jian-Ming Jin*, Shuang-Yan Tang*, Dynamic control of toxic natural product biosynthesis by an artificial regulatory circuit. Metabolic Engineering 2020, 57: 239-246
16. Wei Chen#, Jun Yao#, Jie Meng, Wenjing Han, Yong Tao, Yihua Chen, Yixin Guo, Guizhi Shi, Yang He*, Jian-Ming Jin*, Shuang-Yan Tang*, Promiscuous enzymatic activity-aided multiple-pathway network design for metabolic flux rearrangement in hydroxytyrosol biosynthesis. Nature Communications 2019, 10: 960.
17. Wei Chen#, Xuanxuan Zhang#, Dandan Xiong#, Jian-Ming Jin*, Shuang-Yan Tang*. Engineering the effector specificity of regulatory proteins for the in vitro detection of biomarkers and pesticide residues. Applied Microbiology and Biotechnology 2019, 103: 3205-3213.
18. Heng Li, Jing Li, Ruinan Jin, Wei Chen, Chaoning Liang, Jieyuan Wu, Jian-Ming Jin, Shuang-Yan Tang*, Towards the construction of high-quality mutagenesis libraries. Biotechnology Letters 2018, 40: 1101-1107.
19. Heng Li, Wei Chen, Ruinan Jin, Jian-Ming Jin*, Shuang-Yan Tang*, Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products. Microbial Cell Factories 2017, 16: 187.
20. Heng Li, Chaoning Liang, Wei Chen, Jian-Ming Jin*, Shuang-Yan Tang*, Yong Tao, Monitoring in vivo metabolic flux with a designed whole-cell metabolite biosensor of shikimic acid. Biosensors and Bioelectronics 2017, 98: 457-465.
21. Jieyuan Wu, Peixia Jiang, Wei Chen, Dandan Xiong, Linglan Huang, Junying Jia, Yuanyuan Chen, Jian-Ming Jin*, Shuang-Yan Tang*, Design and application of a lactulose biosensor. Scientific Reports 2017, 7: 45994.
22. Qingzhuo Wang, Shuang-Yan Tang*, Sheng Yang*. Genetic biosensors for small-molecule products: Design and applications in high-throughput screening. Frontiers of Chemical Science and Engineering 2017, 11: 15-26.
23. Dandan Xiong, Shikun Lu, Jieyuan Wu, Chaoning Liang, Wei Wang, Wenzhao Wang, Jian-Ming Jin*, Shuang-Yan Tang*, Improving key enzyme activity in phenylpropanoid pathway with a designed biosensor. Metabolic Engineering 2017, 40: 115-123.
24. Lin Zhu#, Zhe Wu#, Jian-Ming Jin, Shuang-Yan Tang*, Directed evolution of leucine dehydrogenase for improved efficiency of L-tert-leucine synthesis. Applied Microbiology and Biotechnology 2016, 100: 5805-5813.
25. Chaoning Liang#, Yi Zhang#, Yan Jia, Wenzhao Wang, Youhai Li, Shikun Lu, Jian-Ming Jin*, Shuang-Yan Tang*. Engineering a carbohydrate-processing transglycosidase into glycosyltransferase for natural product glycodiversification. Scientific Reports 2016, 6: 21051.
26. Wei Chen#, Shan Zhang#, Peixia Jiang#, Jun Yao, Yongzhi He, Lincai Chen, Xiwu Gui, Zhiyang Dong*, Shuang-Yan Tang*. Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis. Metabolic Engineering 2015, 30:149-155.
27. Peixia Jiang#, Shanshan Mu#, Heng Li, Youhai Li, Congmin Feng, Jian-Ming Jin*, Shuang-Yan Tang*. Design and application of a novel high-throughput screening technique for 1-deoxynojirimycin. Scientific Reports 2015, 5: 8563.
28. Chaoning Liang#, Xiwu Gui#, Cheng Zhou, Yanfen Xue, Yanhe Ma*, Shuang-Yan Tang*. Improving the thermoactivity and thermostability of pectate lyase from Bacillus pumilus for ramie degumming. Applied Microbiology and Biotechnology 2015, 99: 2673-2682.
29. Chaoning Liang#, Dandan Xiong#, Yi Zhang, Shanshan Mu, Shuang-Yan Tang*. Development of a novel uric-acid-responsive regulatory system in Escherichia coli. Applied Microbiology and Biotechnology 2015, 99:2267-2275.
30. Shuang-Yan Tang, Shuai Qian, Olubolaji Akinterinwa, Christopher S. Frei, Joseph A. Gredell, Patrick C. Cirino. Screening for enhanced triacetic acid lactone production by recombinant Escherichia coli expressing a designed triacetic acid lactone reporter. Journal of the American Chemical Society 2013, 135 (27): 10099-10103.
31. Shuang-Yan Tang, Patrick C. Cirino. Design and application of a mevalonate- responsive regulatory protein. Angewandte Chemie International Edition 2011, 50: 1084-1086.
32. Shuang-Yan Tang, Patrick C. Cirino. Elucidating residue roles in engineered variants of AraC regulatory protein. Protein Science 2010, 19: 291-298.
33. Shuang-Yan Tang, Hossein Fazelinia, Patrick C. Cirino. AraC regulatory protein   mutants with altered effector specificity. Journal of the American Chemical Society  2008, 130: 5267-5271.

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5、国家自然科学基金面上项目，改造调控蛋白LacI建立二糖分子高通量筛选方法, 2015/01-2018/12.

6、国家自然科学基金面上项目，改造调控蛋白设计D-塔格糖信号分子及其功能验证，2012/01-2015/12.

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