结合多学科技术手段，解析非编码RNA作用机制，开发基于RNAi的抗病虫作物新品系，创制新型RNAi生物农药。

1.Li, J., Tian, W., Chen, T., Liu, Q.Y., Wu, H.W., Liu, C.H., Fang, Y.Y., Guo, H.S., and Zhao, J.H.* (2025). N(6)-methyladenosine on the natural antisense transcript of NIA1 stabilizes its mRNA to boost NO biosynthesis and modulate stomatal movement. Molecular plant 18, 151-165.

2. Li, J., Zhang, B.S., Wu, H.W., Liu, C.L., Guo, H.S., and Zhao, J.H.* (2024). The RNA-binding domain of DCL3 is required for long-distance RNAi signaling. aBIOTECH 5, 17-28.

3. Zhao, J.-H., Liu, Q.-Y., Xie, Z.-M., and Guo, H.-S. (2024). Exploring the challenges of RNAi-based strategies for crop protection. Advanced Biotechnology 2, 23.

4. 郭惠珊，赵建华主编，小RNA—植物生命过程的关键密码，北京：科学出版社，2023。

5. Liu, Q.Y., Li, Y.C., Wu, H.W., Zhang, B.S., Liu, C.H., Gao, Y., Guo, H.S., and Zhao, J.H.* (2023). Hyphopodium-Specific Signaling Is Required for Plant Infection by. Journal of Fungi 9, 484.

6. Wen, H.G.#, Zhao, J.H.#*, Zhang, B.S., Gao, F., Wu, X.M., Yan, Y.S., Zhang, J., and Guo, H.S.* (2023). Microbe-induced gene silencing boosts crop protection against soil-borne fungal pathogens. Nature plants 9, 1409-1418.

7. Wu, X.M., Zhang, B.S., Zhao, Y.L., Wu, H.W., Gao, F., Zhang, J., Zhao, J.H.*, and Guo, H.S.* (2023). DeSUMOylation of a Verticillium dahliae enolase facilitates virulence by derepressing the expression of the effector VdSCP8. Nat Commun 14, 4844.

8. Zhang, B.S., Li, Y.C., Guo, H.S.*, and Zhao, J.H.*(2022). Verticillium dahliae Secretes Small RNA to Target Host MIR157d and Retard Plant Floral Transition During Infection. Front Plant Sci 13, 847086.

9. Zhao, J.H., and Guo, H.S. (2022). RNA silencing: From discovery and elucidation to application and perspectives. J Integr Plant Biol 64, 476-498.

10. Zhao, J.H., Zhang, T., Liu, Q.Y., and Guo, H.S. (2021). Trans-kingdom RNAs and their fates in recipient cells: advances, utilization, and perspectives. Plant Commun 2, 100167.

11. Cui, C.#, Wang, J.J.#, Zhao, J.H.#, Fang, Y.Y., He, X.F., Guo, H.S., and Duan, C.G. (2020). A Brassica miRNA Regulates Plant Growth and Immunity through Distinct Modes of Action. Molecular plant 13, 231-245.

12. Zhao, J.H., and Guo, H.S. (2019). Trans-kingdom RNA interactions drive the evolutionary arms race between hosts and pathogens. Current opinion in genetics & development 58-59, 62-69.

13. Hua, C.#, Zhao, J.H.#, and Guo, H.S. (2018). Trans-Kingdom RNA Silencing in Plant-Fungal Pathogen Interactions. Molecular plant 11, 235-244.

14. Jin, Y., Zhao, P., Fang, Y.Y., Gao, F., Guo, H.S.*, and Zhao, J.H.*(2018). Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots. Mycology 9, 155-165.

15. Zhao, J.H.*, Liu, X.L., Fang, Y.Y., Fang, R.X., and Guo, H.S.* (2018). CMV2b-Dependent Regulation of Host Defense Pathways in the Context of Viral Infection. Viruses 10, 618.

16. Zhang, T.#, Zhao, Y.L.#, Zhao, J.H.#, Wang, S., Jin, Y., Chen, Z.Q., Fang, Y.Y., Hua, C.L., Ding, S.W., and Guo, H.S. (2016). Cotton plants export microRNAs to inhibit virulence gene expression in a fungal pathogen. Nature plants 2, 16153.

17. Zhao, J.H.*, Fang, Y.Y., Duan, C.G., Fang, R.X., Ding, S.W., and Guo, H.S.* (2016). Genome-wide identification of endogenous RNA-directed DNA methylation loci associated with abundant 21-nucleotide siRNAs in Arabidopsis. Scientific reports 6, 36247.

18. Fang, Y.Y.#, Zhao, J.H.#, Liu, S.W., Wang, S., Duan, C.G., and Guo, H.S. (2016). CMV2b-AGO Interaction Is Required for the Suppression of RDR-Dependent Antiviral Silencing in Arabidopsis. Frontiers in microbiology 7, 1329.

19. Zhao, J.H., Hua, C.L., Fang, Y.Y., and Guo, H.S. (2016). The dual edge of RNA silencing suppressors in the virus-host interactions. Curr Opin Virol 17, 39-44.

20. Zahid, K.#, Zhao, J.H.#, Smith, N.A., Schumann, U., Fang, Y.Y., Dennis, E.S., Zhang, R., Guo, H.S., and Wang, M.B. (2015). Nicotiana small RNA sequences support a host genome origin of cucumber mosaic virus satellite RNA. PLoS genetics 11, e1004906.

1、国家自然科学基金青年项目，反向长链非编码RNA调控NIA基因响应CMV侵染的机制研究，2017/01-2019/12

2、国家自然科学基金面上项目，LncRNA、m6A甲基化和RNAi协同调控NIA1基因表达分子机制研究，2022/01-2025/12

3、国家自然科学基金面上项目，tRNA-like RNA介导的真菌种间基因表达调控机制及应用研究，2026/01-2029/12

4、中国科学院战略性先导科技专项（A类），靶向型抗病RNA干扰菌剂研制，2021/01-2025/12

5、中国科学院战略性先导科技专项（A类），甘蔗HIGS抗病育种，2023/01-2028/12

6、生物互作卓越创新中心课题，致病真菌V.dahlia内源新型基因编辑系统鉴定，2021/01-2021/12

7、中国科学院微生物研究所所长改革创新基金，不依赖Cas酶的真核微生物基因编辑系统鉴定，2020/11-2022/10

8、黑龙江省重点研发项目，RNAi 靶标筛选及工程菌株构建，2025/01-2028/01