产甲烷古菌资源、生理及其代谢途径。近期研究聚焦稻田砷污染以及甲烷减排。研究发现新型甲基产甲烷途径：合作揭示稻田细菌与古菌协同代谢二甲基砷产甲烷新途径, 并有效减轻水稻直穗病；进一步解
析稻田电子还原甲基包括甲基砷产甲烷机制，为稻田甲烷减排提供策略依据；发现厌氧低温泥炭地木质素来源产甲烷途径，为气候变暖提供预警；揭示冷泉细菌与古菌协同代谢甜菜碱产甲烷途径。

1.Li Lingyan*#, Zhang Wenting#, Zhang Shengjie#, Song Lei, Sun Qinglei, Zhang Huan, Xiang Hua, Dong Xiuzhu*. Bacteria and archaea synergistically convert glycine betaine to biogenic methane in the formosa cold seep of the South China Sea. mSystems.2021.6(5):e0070321.

2.Liu Tuo#, Li Lingyan#, Xue kai#, Wang xuemeng, Li Huiju, Wang Yanfen*, Dong Xiuzhu*. Methane production from lignin in anoxic peatland.Nature Geoscience.2025.DOI:10.1038/s41561-025-01758-5.

3.Chen Chuan#, Li Lingyan#, Wang Yanfen, Dong Xiuzhu*, Zhao Fangjie*. Methylotrophic methanogens and bacteria synergistically demethylate dimethylarsenate in paddy soil and alleviate rice straighthead disease.ISME Journal.2023.17(11):1851-1861.

4.Li Lingyan#, Tian Xuping#, Wang Xuemeng, Chen Chuan, Zhou Qi, Qi Lei, Li Jie, Xue Kai, Zhao Fangjie, Wang Yanfen*, Dong Xiuzhu*. Extracellular electron transfer based methylotrophic methanogenesis in paddy soil and the prevalent Methanomassiliicoccus.Communications Earth & Environment.2025.6:297.

5.Chen Lin#, Li Lingyan#, Zhang Shengjie, Zhang Wenting, Xue Kai, Wang Yanfen*, Dong Xiuzhu*. Anaerobic methane oxidation linked to Fe (III) reduction in a Candidatus Methanoperedens-enriched consortium from the cold Zoige wetland at Tibetan Plateau. Environmental Microbiology.2021.24(2):614-625.

6.Li Lingyan#, Ren Mifang#, Xu Yueqiang, Jin Cheng, Zhang Wenhao, Dong Xiuzhu*. Enhanced glycosylation of an S-layer protein enables a psychrophilic methanogenic archaeon to adapt to elevated temperatures in abundant substrates. FEBS Letters.2019.594(4):665-677.

7.Li Lingyan#, Zheng Mingyue#, Ma Hailing, Gong Shufen, Ai Guomin, Liu Xiaoli, Li Jie, Wang Kaijun*, Dong Xiuzhu*. Significant performance enhancement of a UASB reactor by using acyl homoserine lactones to facilitate the long filaments of Methanosaeta harundinacea 6Ac. Applied Microbiology and Biotechnology.2015.99(15):6471-6480.

1、国家自然科学基金委员会, 面上项目, 3257010706, 稻田胞外电子驱动甲基还原产甲烷机制研究, 2026-01-01 至 2029-12-31, 50万元。

2、国家自然科学基金委员会, 青年科学基金项目, 31700046, 嗜冷古菌R15“进化”为中温菌的糖蛋白组学研究, 31700046, 2018-01-01 至 2020-12-31, 24万元。

3、中国科学院仪器设备功能开发技术创新项目,智能厌氧工作站研发, 2019000071, 2019-08-01 至 2021-08-01, 30万元。

4、科技部项目,青藏高原二次科考, 2019QZKK0304-02, 典型高寒湿地碳固持过程与机理, 2019QZKK0304-02, 2022-11至2024-10, 15万元。

5、中国科学院地球化学研究所,环境地球化学国家重点实验室开放课题, SKLEG2024207, 稻田产甲烷古菌介导的甲基汞脱甲基过程及其机制研究, 2024-4至2024-12, 4万元。

6、滨州魏桥国科项目, 区域生态系统增汇与温室气体减排集成技术研究, GYY-NYHJ-2023-WT-005, 2023-10至2025-12, 40万元。