代表性图片

Toxin-antitoxin RNA pair CreTA safeguards CRISPR-Cas.

Science, 2021,372(6541)

Three steps and the delicate molecular specificities during primed CRISPR adaptation.

(Nucleic Acids Res. 2014,42(4): 2483-92;Nucleic Acids Res. 2014, 42(11): 7226-35;

Nucleic Acids Res.2016, 44(9): 4266–77;Nucleic Acids Res.2017, 45(8): 4642-54)

 

 

近期代表性论文（* 通讯作者）

　[2021]

　　Li M, Gong L, Cheng F, Yu H, Zhao D, Wang R, Wang T, Zhang S, Zhou J, Shmakov SA, Koonin EV,Xiang H*.Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.Science. 2021; 372(6541): abe5601. Doi: 10.1126/science.abe5601

　　Xue Q, Zhao D, Zhang S, Zhou H, Zuo Z, Zhou J, Li M,Xiang H*. Highly integrated adaptive mechanisms inSpiribacter halalkaliphilus, a bacterium abundant in Chinese soda-saline lakes.Environ Microbiol. 2021, Doi: 10.1111/1462-2920.15794

　　Lin L, Chen J, Mitra R, Gao Q, Cheng F, Xu T, Zuo Z,Xiang H*,Han J*. Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux. Commun Biol. 2021;4(1):1007. Doi: 10.1038/s42003-021-02541-z

　　Zuo Z, Zhao D, Zhou J, Han J,Xiang H*.Halalkalirubrum salinumgen. nov., sp. nov., a halophilic archaeon isolated from a saline lake.Antonie Van Leeuwenhoek.2021;114(1):83-94. Doi: 10.1007/s10482-020-01502-6

　　Xue Q, Zuo Z, Zhou H, Zhou J, Zhang S, Han J, Zhao D，Xiang H*.Salinadaptatus halalkaliphilusgen. nov., sp. nov., a haloalkaliphilic archaeon isolated from salt pond in Inner Mongolia Autonomous Region, China.Int J Syst Evol Microbiol. 2021;71(1). Doi: 10.1099/ijsem.0.004584

　　Xue Q, Zhao D, Zuo Z, Zhou J,Xiang H*.Natronorubrum halalkaliphilumsp. nov., a haloalkaliphilic archaeon isolated from soda lake in Inner Mongolia Autonomous Region, China.Arch Microbiol. 2021. Doi: 10.1007/s00203-021-02207-8

　　Xu Z, Li Y, Li M,Xiang H, Yan A. Harnessing the type I CRISPR-Cas systems for genome editing in prokaryotes.Environ Microbiol. 2021;23(2):542-58. Doi: 10.1111/1462-2920.15116

　　Mitra R, Xu T, Chen GQ,Xiang H*, Han J. An updated overview on the regulatory circuits of polyhydroxyalkanoates synthesis.Microb Biotechnol.2021. Doi: 10.1111/1751-7915.13915

　　Li Y, Yu H, Guan H, Li J, Zhang J,Xiang H, Li J, Tan H. Activation of Cryptic Antibiotic Biosynthetic Gene Clusters Guided by RNA-seq Data from Both Streptomyces ansochromogenes and DeltawblA.Antibiotics(Basel). 2021;10(9). Doi: 10.3390/antibiotics10091097

　　Li L, Zhang W, Zhang S, Song L, Sun Q, Zhang H,Xiang H, Dong X. Bacteria and Archaea Synergistically Convert Glycine Betaine to Biogenic Methane in the Formosa Cold Seep of the South China Sea.mSystems.2021:e0070321. Doi: 10.1128/mSystems.00703-21

　　Cheng F, Wang R, Yu H, Liu C, Yang J,Xiang H*, Li M. Divergent degeneration ofcreAantitoxin genes from minimal CRISPRs and the convergent strategy of tRNA-sequestering CreT toxins.Nucleic Acids Res.2021;49(18):10677-88. Doi: 10.1093/nar/gkab821

　　Chen S, Sun S, Wang R, Feng H,Xiang H*. Halolysin R4 of Haloferax mediterranei confers its host antagonistic and defensive activities.Appl Environ Microbiol.2021. Doi: 10.1128/AEM.02889-20

　　[2020]

　　Zhao D, Zhang S, Xue Q, Chen J, Zhou J, Cheng F,Li M, Zhu Y, Yu H, Hu S, Zheng Y, Liu S,Xiang H*. (2020). Abundant Taxa and Favorable Pathways in the Microbiome of Soda-Saline Lakes in Inner MongoliaFront Microbiol, 10.3389/fmicb.2020.01740

　　Liu W, An C, Shu X, Meng X, Yao Y, Zhang J, Chen F,Xiang H, Yang S, Gao X, Gao SS. (2020). A Dual-Plasmid CRISPR/Cas System forMycotoxin Elimination in Polykaryotic Industrial Fungi.ACS Synth Biol. doi: 10.1021/acssynbio.0c00178.

　　Xu Z, Li Y, Li M,Xiang H, Yan A. (2020). Harnessing the type I CRISPR-Cas systems for genome editing in prokaryotes.Environ Microbiol.doi: 10.1111/1462-2920.15116.

　　Zhou S,Xiang H, Liu JL. (2020). CTP synthase forms cytoophidia in archaea.J Genet Genomics.47(4):213-223. doi: 10.1016/j.jgg.2020.03.004.

　　Kumar S, Zhou J, Li M,Xiang H*, Zhao D*. (2020). Insights into the metabolism pathway and functional genes of long-chain aliphatic alkane degradation in haloarchaea.Extremophiles. doi:10.1007/s00792-020-01167-z

　　Mitra R, Xu T,Xiang H*, Han J*. (2020). Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory.Microb Cell Fact, 19(1), 86. doi:10.1186/s12934-020-01342-z

　　Liu C, Zhou N, Du MX, Sun YT, Wang K, Wang YJ, . . .Xiang H，etal. (2020). The Mouse Gut Microbial Biobank expands the coverage of cultured bacteria.Nat Commun, 11(1), 79. doi:10.1038/s41467-019-13836-5

　　Lu H, Pei C, Zhou H, Lü Y, He Y, Li Y, Han J,Xiang H, Eichler J, Jin C. (2020). Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaonHaloarcula hispanica.Mol Microbiol. (in press)

　　Johnsen U, Sutter JM, Reinhardt A, Pickl A, Wang R,Xiang H, Schonheit P (2020). D-Ribose Catabolism in Archaea: Discovery of a Novel Oxidative Pathway in Haloarcula Species.JBacteriol, 202(3). doi:10.1128/JB.00608-19

　　Chen J, Mitra R,XiangH*, Han J 2020. Deletion of thepps-like gene activates the crypticphaCgenes inHaloferax Mediterranei.Appl Microbiol Biotechnol. doi:10.1007/s00253-020-10898-0

　　[2019]

　　Xu Z, Li M, Li Y, Cao H, Miao L, Xu Z, Higuchi Y, Yamasaki S, Nishino K, Woo PCY,Xiang H*, Yan A. 2019. Native CRISPR-Cas-Mediated Genome Editing Enables Dissecting and Sensitizing Clinical Multidrug-Resistant P. aeruginosa.Cell Rep. 29(6):1707-1717.e3. doi: 10.1016/j.celrep.2019.10.006.

　　Chen S, Sun S, Korfanty GA, Liu J,Xiang H*. 2019. A Halocin Promotes DNA Uptake in Haloferax mediterranei.Front Microbiol.doi: 10.3389/fmicb.2019.01960.

　　Chen J, Mitra R, Zhang S, Zuo Z, Lin L, Zhao D,Xiang H*, Han J. 2019. Unusual Phosphoenolpyruvate (PEP) Synthetase-Like Protein Crucial to Enhancement of Polyhydroxyalkanoate Accumulation in Haloferax mediterranei Revealed by Dissection of PEP-Pyruvate Interconversion Mechanism.Appl Environ Microbiol. 2019 Sep 17;85(19). pii: e00984-19. doi: 10.1128/AEM.00984-19.

　　Gong L, Li M, Cheng F, Zhao D, Chen Y,Xiang H*. 2019. Primed adaptation tolerates extensive structural and size variations of the CRISPR RNA guide in Haloarcula hispanica.Nucleic Acids Res.47(11):5880-5891. doi: 10.1093/nar/gkz244.

　　Huang Z, Wang YH, Zhu HZ, Andrianova EP, Jiang CY, Li D, Ma L, Feng J, Liu ZP,Xiang H, Zhulin IB, Liu SJ. 2019. Cross Talk between Chemosensory Pathways That Modulate Chemotaxis and Biofilm Formation.mBio. 2019 Feb 26;10(1). pii: e02876-18. doi: 10.1128/mBio.02876-18.

　　You L, Ma J, Wang J, Artamonova D, Wang M, Liu L,Xiang H, Severinov K, Zhang X, Wang Y. 2019. Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference.Cell. 176(1-2):239-253.e16. doi: 10.1016/j.cell.2018.10.052.

　　[2018]

　　Guo Z, Li P, Chen G, Li C, Cao Z, Zhang Y, Ren J,Xiang H, Lin S, Ju J, Chen Y. 2018. Design and Biosynthesis of Dimeric Alboflavusins with Biaryl Linkages via Regiospecific C-C Bond Coupling.J Am Chem Soc. 140(51):18009-18015. doi: 10.1021/jacs.8b10136..

　　Zuo ZQ, Xue Q, Zhou J, Zhao DH, Han J,Xiang H*. 2018. Engineering Haloferax mediterranei as an Efficient Platform for High Level Production of Lycopene.Front Microbiol.9:2893. doi: 10.3389/fmicb.2018.02893.

　　Xue Q, Liu XB, Lao YH, Wu LP, Wang D, Zuo ZQ, Chen JY, Hou J, Bei YY, Wu XF, Leong KW,Xiang H, Han J. 2018. Anti-infective biomaterials with surface-decorated tachyplesin I.Biomaterials. 178:351-362. doi: 10.1016/j.biomaterials.2018.05.008.

　　Li M, Cheng FY, Gong LY,Xiang H*.2018.[Systematic discovery of novel prokaryotic defense systems: progress and prospects].Yi Chuan.40(4):259-265. doi: 10.16288/j.yczz.18-075.

　　[2017]

　　Cheng F, Gong L, Zhao D, Yang H, Zhou J, Li M*,Xiang H*.2017. Harnessing the native type I-B CRISPR-Cas for genome editing in a polyploid archaeon.J Genet Genomics.44(11):541-548.

　　Zhao D,Yang H,Chen J,Cheng F,Kumar S,Han J,Li M,Zhou J,Xiang H*. 2017. Development of the first gene expression system forSalinicoccusstrains with potential application in bioremediation of hypersaline wastewaters.Appl Microbiol Biotechnol.101(19):7249-7258.

　　Zhao D,Kumar S,Zhou J,Wang R,Li M,Xiang H*. 2017. Isolation and complete genome sequence ofHalorientalis hydrocarbonoclasticussp. nov., a hydrocarbon-degrading haloarchaeon. Extremophiles. 21(6):1081-1090.

　　Liu J, Wang Q, Jiang X, Yang H, Zhao D, Han J, Luo Y,Xiang H*.2017. Systematic analysis of Lysine acetylation in the halophilic archaeonHaloferax mediterranei.J Proteome Res.16(9):3229-3241.

　　Han J, Wu L, Liu X, Hou J, Zhao L, Chen J, Zhao D,Xiang H*.2017. Biodegradation and biocompatibility of haloarchaea-produced poly(3-hydroxybutyrate-co-3 -hydroxyvalerate) copolymers.Biomaterials.139, 172-186.

　　Li M, Gong L., Zhao D, Zhou J,Xiang H*.2017. The spacer size of I-B CRISPR is modulated by the terminal sequence of the protospacer.Nucleic Acids Research.45, 4642-4654.

　　Borjian F, Han J, Hou J,Xiang H,Zarzycki J, Berg IA. 2017. Malate synthase and beta-methylmalyl coenzyme a lyase reactions in the methylaspartate cycle inHaloarcula hispanica.J Bacteriol.199, pii: e00657-16. doi: 10.1128/JB.00657-16.

　　[2016]

　　Wang R, Li M, Gong L, Hu S,Xiang H*. 2016. DNA motifs determining the accuracy of repeat duplication during CRISPR adaptation in Haloarcula hispanica.Nucleic Acids Research, 44(9):4266-77

　　Liu G, Cai S, Hou J, Zhao D, Han J, Zhou J,Xiang H*. 2016. Enoyl-CoA hydratase mediates polyhydroxyalkanoate mobilization in Haloferax mediterranei.Scientific Reports. 6, 24015

　　Borjian F, Han J, Hou J,Xiang H, Berg IA. 2016. The methylaspartate cycle in haloarchaea and its possible role in carbon metabolism.ISME Journal, 10(3):546-57

　　Liu X, Wu L, Hou J, Chen J, Han J*,Xiang H*. 2016. Environmental biodegradation of haloarchaea-produced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in activated sludge.Applied Microbiology and Biotechnology,100(15):6893-6902

　　Chen S, Liu HC, Zhou J, Xiang H*. 2016. Haloparvum sedimenti gen. nov., sp. nov., a new member of the family Haloferacaceae.Int J Syst Evol Microbiol.66(6):2327-34.

　　Chen S, Liu HC, Zhou J,Xiang H*. 2016. Halorubrum pallidum sp. nov., a novel extremely halophilic archaeon isolated from a subterranean rock salt.Int J Syst Evol Microbiol. 66(8):2980-6

　　Chen S, Wang C,Xiang H. 2016. Sequence analysis and minimal replicon determination of a new haloarchaeal plasmid pHF2 isolated from Haloferax sp. strain Q22.Plasmid. 83:1-7.

　　Xiang H*.2016. PHBHV Biosynthesis byHaloferax mediterranei: from Genetics, Metabolism, and Engineering to Economical Production.Recent Advances in Biotechnology(Martin Koller Ed. Bentham Science Publishers),2016, 348-379

　　[2015]

　　Lu H, Lü Y, Ren J, Wang Z, Wang Q, Luo Y, Han J,Xiang H, Du Y, Jin C. 2015. Identification of the S-layer glycoproteins and their covalently linked glycans in the halophilic archaeon Haloarcula hispanica.Glycobiology.2015, 25(11):1150-62.

　　Yang H, Wu Z, Liu J, Liu X, Wang L, Cai S,Xiang H*.2015. Activation of a dormant replication origin is essential for Haloferax mediterranei lacking the primary origins.Nat Commun.2015, 6:8321.

　　Liu G, Hou J, Cai S, Zhao D, Cai L, Han J, Zhou J,Xiang H*. 2015. A patatin-like protein associated with the polyhydroxyalkanoate (PHA) granules ofHaloferax mediterranei acts as an efficient depolymerase in the degradation of native PHA.Appl Environ Microbiol.2015 , 81(9):3029-38.

　　Han J, Wu LP, Hou J, Zhao D,Xiang H*. 2015. Biosynthesis, characterization, and hemostasis potential of tailor-made poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Haloferax mediterranei.Biomacromolecules.2015, 16(2):578-88.

　　Hou J,Xiang H*, Han J. 2015.Propionyl coenzyme A (propionyl-CoA) carboxylase in Haloferax mediterranei: Indispensability for propionyl-CoA assimilation and impacts on global metabolism.Appl Environ Microbiol.2015, 81(2):794-804.

　　Cai S, Cai L, Zhao D, Liu G, Han J, Zhou J,Xiang H*. 2015. A novel DNA-binding protein, PhaR, plays a central role in the regulation of polyhydroxyalkanoate accumulation and granule formation in the haloarchaeon Haloferax mediterranei.Appl Environ Microbiol. 2015, 81(1):373-85.

　　[2014]

　　Wu Z, Yang H, Liu J, Wang L,Xiang H*. 2014. Association between the Dynamics of Multiple Replication Origins and the Evolution of Multireplicon Genome Architecture in Haloarchaea.Genome Biology and Evolution, 26(10):2799-810.

　　Li M, Wang R,Xiang H*.2014.Haloarcula hispanicaCRISPR authenticates PAM of a target sequence to prime discriminative adaptation.Nucleic Acids Research, 42(11):7226-35

　　Cai L, Cai S, Zhao D, Wu J, Wang L, Liu X, Li M, Hou J, Zhou J, Liu J, Han J,Xiang H*. 2014. Analysis of the transcriptional regulator GlpR, promoter elements, and posttranscriptional processing involved in fructose-induced activation of the phosphoenolpyruvate-dependent sugar phosphotransferase system inHaloferax mediterranei.Applied andEnvironmental Microbiology,80(4):1430-40.

　　Wu Z, Liu J, Yang H, Liu H,Xiang H*.2014. Multiple replication origins with diverse control mechanisms inHaloarcula hispanica.Nucleic Acids Research,42(4): 2282-2294.

　　Li M, Wang R, Zhao D,Xiang H*. 2014. Adaptation of theHaloarcula hispanicaCRISPR-Cas system to a purified virus strictly requires a priming process.Nucleic Acids Research,42(4): 2483-2492.

　　Wu Z, Liu J, Yang H,Xiang H*.2014. DNA replication origins in archaea.Frontiers in Microbiology,5:179.

　　Hou J, Han J, Cai L, Zhou J, LüY, Jin C, Liu J,Xiang H*. 2014. Characterization of genes for chitin catabolism inHaloferax mediterranei.AppliedMicrobiology and Biotechnology,98(3): 1185-1194

　　[2013]

　　Hou J, Feng B, Han J, Liu H, Zhao D, Zhou J,Xiang H*. 2013. HaloarchaealType β-Ketothiolases Involved in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Synthesis in Haloferax mediterraneiApplied and Environmental Microbiology,79(17):5104-11.

　　Han J, Hou J, Zhang F, Ai G, Li M, Cai S, Liu H, Wang L, Wang Z, Zhang S, Cai L, Zhao D, Zhou J,Xiang H*.2013.Multiple Propionyl-CoA Supplying Pathways for Production of BioplasticPoly(3-hydroxybutyrate-co-3-hydroxyvalerate) inHaloferax mediterranei.Applied andEnvironmental Microbiology,79(9): 2922-31.

　　Liu X, Wang L, Liu J, Cai L,Xiang H*. 2013. Genome-wide analysis of gene expression in stationary phase and genetic characterization of stationary-phase-dependent halocin gene expression in the haloarchaeonHaloferax mediterranei.Journal ofGenetics and Genomics, 40: 441-444.

　　Liu H, Luo Y, Han J, Wu J, Wu Z, Feng D, Cai S, Li M, Liu J, Zhou J,Xiang H*. 2013. Proteome reference map ofHaloarcula hispanicaand comparativeproteomic and transcriptomic analysis of polyhydroxyalkanoate biosynthesisunder genetic and environmental perturbations.Journal of Proteome Research,12(3):1300-15.

　　Li M, Liu H, Han J, Liu J, Wang R, Zhao D, Zhou J,Xiang H*. 2013. Characterization of CRISPR RNA biogenesis and Cas6-cleavage mediated inhibitionof a provirus in the haloarchaeonHaloferax mediterranei.Journal ofBacteriology, 195(4):867-75.

　　Liu X, Miao D, Zhang F, Wu Z, Liu J,Xiang H*.2013. Characterizationof the minimal replicon of pHM300 and independent copy number control of majorand minor chromosomes ofHaloferaxmediterranei.FEMS MicrobiologyLetters,339(1):66-74.

　　Zhao D, Cai L, Wu J, Li M, Liu H, Han J, Zhou J,Xiang H*.2013. Improving polyhydroxyalkanoateproduction by knocking out the genes involved in exopolysaccharide biosynthesisin Haloferax mediterranei.AppliedMicrobiology and Biotechnology,97(7):3027-36.

　　[2012]

　　Wu Z, Liu H, Liu J, Liu X,Xiang H*. 2012. Diversity and evolution of multiple orc/cdc6-adjacent replication origins in haloarchaea,BMC Genomics.13: 478.

　　Liu J, Li J, Wu Z, Pei H, Zhou J,Xiang H*. 2012. Identification and Characterization of the Cognate Anti-Sigma Factor and Specific Promoter Elements of a T. tengcongensis ECF Sigma Factor.PLoS One7(7): e40885.

　　Han J, Zhang F, Hou J, Liu X, Li M, Liu H, Cai L, Zhang B, Chen Y, Zhou J, Hu S,Xiang H*. 2012. Complete genome sequence of the metabolically versatile halophilic archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) producer.Journal of Bacteriology, 194 (16): 4463–4464.

　　Cai L, Zhao D, Hou J, Wu J, Cai S, DasSarma P,Xiang H*. 2012. Cellular and organellar membrane-associated proteins in haloarchaea: perspectives on physiological significance and biotechnological application.Science China Life Science, 55: 404–414.

　　Cai S, Cai L, Liu H, Liu X, Han J, Zhou J,Xiang H*.2012. Identification of the haloarchaeal type phasin (PhaP) that functions in polyhydroxyalkanoate accumulation and granule formation inHaloferax mediterranei.Applied and Environmental Microbiology,78(6):1946-1952.

申请或授权专利

　　1.向华，周梅先等《一株嗜盐碱杆菌及其专有质粒与应用》

　　（专利号：ZL02100714.4），2004.11.24授权。

　　2.向华，孙超岷《一种极端嗜盐古菌质粒及其衍生质粒载体》

　　 （专利号：ZL2004100497519），2008.10.1授权

　　3.向华，任媛媛等《一种热稳定海藻糖合成酶及其编码基因与应用》

　　 （专利号：ZL200410073883.5），2007.11.7授权。

　　4.向华，厉云等《一种嗜盐菌素及其免疫蛋白和它们的编码基因与应用》

　　 （专利号：ZL200510055640.3），2009.2.18授权。

　　5.向华，周利刚等《表达极端嗜盐古菌紫膜蛋白突变体的载体及工程菌》

　　 （专利号：ZL200510087721.1），2007.4.4授权。

　　6.向华，韩静等《极端嗜盐古菌聚羟基脂肪酸酯合酶及其编码基因与应用》

　　 (专利号：ZL200710119986.4)，2012年1月4日授权。

　　7.向华，陆秋鹤等《一种与聚羟基丁酸戊酸共聚酯合成相关的酶及其编码基因与应用》

　　 （专利号：ZL200810101529），2011年7月27日授权。

　　8.向华，冯博等《一种与PHBV的3-HV单体合成相关的β-酮硫解酶及其编码基因与应用》

　　（专利号：ZL201010195738.X），2012年4月3日授权。

　　9.向华，刘海龙《一种基于地中海富盐菌和pyrF基因的遗传操作系统及其应用》

　　(专利号：ZL201110054456.2)），2014年1月1日授权。

　　10.向华，刘海龙《一种基于西班牙盐盒菌和pyrF基因的遗传操作系统及其应用》

　　(专利号：ZL201110054379.0)，2014年3月26日授权。

　　11.向华，赵大贺《高效利用碳源生产生物塑料PHBV的极端嗜盐古菌工程菌》

　　(专利号：ZL2012 1 0181052.4)， 2015年6月10日授权

　　12.韩静，向华，侯靖.国家发明专利《一种PHBV聚合物、制备方法及利用PHBV聚合物制作的止血材料》（ZL 2014 1 0658043.9）,2017年1月4日授权

　　13.韩静，向华，侯靖.国家发明专利《PHBV共聚物的制备方法及由此制备的PHBV共聚物》（申请号：201410699653.3）

　　14.向华，刘桂明，韩静，赵大贺《一种聚羟基烷酸颗粒降解酶其用途及(R)-3HB生产方法》(专利申请号：201510056342.X)

　　15.赵大贺，周坚，向华《一株耐高盐耐高COD盐水球菌菌株和来源该菌株的内源质粒》（专利申请号：201610951864.0）

　　16. 赵大贺，向华，周坚，苏米特？库玛 《一株在高盐条件下降解烷烃的盐东方菌菌株及其培养方法》（专利申请号：201710199115.1）

　　17. 韩静，向华，刘晓斌，薛琼《一种PhaP-Tac的微生物合成及其对疏水材料PHBV的修饰方法》（申请号：201810417080.9）