代表性成果

近期代表性论文(* 通讯作者)

2021 

1. Li S, Yang B, Tan G-Y, Ouyang L-M, Qiu S, Wang W*, Xiang W*, Zhang L*: Polyketide pesticides from actinomycetes. Current Opinion in Biotechnology 2021, 69:299-307.

2. Yang Y., Sun Q., Liu Y., Yin H., Yang W., Wang Y., Liu Y., Li Y., Pang S., Liu W., Zhang Q, Yuan F, Qiu S., Li J., Wang X., Fan K., Wang W., Li Z.*, Yin S*. Development of a pyrF-based counterselectable system for targeted gene deletion in Streptomyces rimosus, Journal of Zhejiang University SCIENCE B. 2021, 22:383-396.

3. Zhao X.#, Li S.#, Liu G.#, Wang Z.#, Yang Z., Zhang Q., Liang M., Liu J., Li Z., Tong Y., Zhu G., Wang X., Jiang L., Wang W.*, Tan G-Y*, Zhang L.*: A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes. Sci Bull 2021, 66:69-77。

4.  

2020 

5. Li S., Li Z., Xiang W., Pang S., Wang W.*: Coordinating precursor supply for pharmaceutical polyketide production in Streptomyces. Curr Opin Biotechnol 2020, 69:26-34.

6. Cao Z., Yu J., Wang W., Lu H., Xia X., Xu H., Yang X., Bao L., Zhang Q., Wang H., Zhang S., Zhang L.*: Multi-scale data-driven engineering for biosynthetic titer improvement. Curr Opin Biotechnol 2020, 65:205-212.

7. Zhang Q., Ren J. W., Wang W., Zhai J., Yang J., Liu N., Huang Y., Chen Y.*, Pan G.*, Fan K.*: A Versatile Transcription-Translation in One Approach for Activation of Cryptic Biosynthetic Gene Clusters. ACS Chem Biol 2020.

8. Yang Z#, Sun Q#, Tan G, Zhang Q, Wang Z, Li C, Qi F, Wang W*, Zhang L*, Li Z*: Engineering thermophilic Geobacillus thermoglucosidasius for riboflavin production. Microbial Biotechnology 2020, n/a(n/a). doi:10.1111/1751-7915.13543

9. Wang W#,*,, Li S#, Li Z#, Zhang J#, Fan K, Tan G, Ai G, Lam SM, Shui G, Yang Z, Lu H, Jin P, Li Y, Chen X, Xia X, Liu X, Dannelly HK, Yang C, Yang Y, Zhang S, Alterovitz G, Xiang W*, Zhang L*: Harnessing the intracellular triacylglycerols for titer improvement of polyketides in Streptomyces. Nat Biotechnol 2020. 38(1):76-83.

 

2019 

10. Yin H, Wang W, Fan K, Li Z*: Regulatory perspective of antibiotic biosynthesis in Streptomyces. Sci China Life Sci 2019, 62(5):698-700.

11. Wang X#, Yin S#, Bai J, Liu Y, Fan K, Wang H, Yuan F, Zhao B*, Li Z*, Wang W*: Heterologous production of chlortetracycline in an industrial grade Streptomyces rimosus host. Appl Microbiol Biotechnol 2019, 103(16):6645-6655.

12. Liang M#, Li Z#, Wang W#, Liu J#, Liu L, Zhu G, Karthik L, Wang M, Wang KF, Wang Z, Yu J, Shuai Y, Yu J, Zhang L, Yang Z, Li C, Zhang Q, Shi T, Zhou L, Xie F, Dai H, Liu X, Zhang J, Liu G, Zhuo Y, Zhang B, Liu C, Li S, Xia X, Tong Y, Liu Y, Alterovitz G, Tan G*, Zhang L*: A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules. Nat Commun 2019, 10(1):3672.

13. Wang J#, Wang W, Wang H, Yuan F, Xu Z, Yang K, Li Z*, Chen Y*, Fan K*: Improvement of stress tolerance and riboflavin production of Bacillus subtilis by introduction of heat shock proteins from thermophilic bacillus strains. Appl Microbiol Biotechnol 2019, 103(11):4455-4465.

 

2018  

14. Cao J#, Yao Y#, Fan K, Tan G, Xiang W, Xia X, Li S*, Wang W*, Zhang L*: Harnessing a previously unidentified capability of bacterial allosteric transcription factors for sensing diverse small molecules in vitro. Sci Adv 2018, 4(11):eaau4602.

15. Yao Y#, Li S#, Cao J, Liu W, Fan K, Xiang W, Yang K*, Kong D*, Wang W*: Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification. Chem Commun (Camb) 2018, 54(38):4774-4777.

16. Yao Y#, Li S#, Cao J, Liu W, Qi F, Xiang W, Yang K, Wang W*, Zhang L*: A novel signal transduction system for development of uric acid biosensors. Appl Microbiol Biotechnol 2018, 102(17):7489-7497.

17. Li S#, Wang J#, Xiang W, Yang K, Li Z*, Wang W*: An Autoregulated Fine-Tuning Strategy for Titer Improvement of Secondary Metabolites Using Native Promoters in Streptomyces. ACS Synth Biol 2018, 7(2):522-530.

 

2017  

18. Li X#, Wang J, Shi M, Wang W, Corre C, Yang K*: Evidence for the formation of ScbR/ScbR2 heterodimers and identification of one of the regulatory targets in Streptomyces coelicolor. Appl Microbiol Biotechnol 2017, 101(13):5333-5340.

19. Li S#, Zhou L, Yao Y, Fan K, Li Z, Zhang L, Wang W*, Yang K*: A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays. Chem Commun (Camb) 2017, 53(1):99-102.

20. Yin S#, Wang X#, Shi M, Yuan F, Wang H, Jia X, Yuan F, Sun J, Liu T, Yang K, Zhang Y*, Fan K*, Li Z*: Improvement of oxytetracycline production mediated via cooperation of resistance genes in Streptomyces rimosus. Sci China Life Sci 2017, 60(9):992-999.

 

2016  

21.  Wang W#, Yang T#, Li Y, Li S, Yin S, Styles K, Corre C, Yang K*: Development of a Synthetic Oxytetracycline-Inducible Expression System for Streptomycetes Using de Novo Characterized Genetic Parts. ACS Synth Biol 2016, 5(7):765-773.

22. Yin S#, Li Z#, Wang X, Wang H, Jia X, Ai G, Bai Z, Shi M, Yuan F, Liu T, Wang W*, Yang K*: Heterologous expression of oxytetracycline biosynthetic gene cluster in Streptomyces venezuelae WVR2006 to improve production level and to alter fermentation process. Appl Microbiol Biotechnol 2016, 100(24):10563-10572.

23. Jiang T#, Ke B, Chen H, Wang W, Du L, Yang K, Li M*: Bioluminescent Probe for Detecting Mercury(II) in Living Mice. Anal Chem 2016, 88(15):7462-7465.

24. Sidda JD#, Poon V, Song L, Wang W, Yang K, Corre C*: Overproduction and identification of butyrolactones SCB1-8 in the antibiotic production superhost Streptomyces M1152. Org Biomol Chem 2016, 14(27):6390-6393.

25. Jiang T#, Wang W, Wu X, Wu W, Bai H, Ma Z, Shen Y, Yang K*, Li M*: Discovery of New Substrates for LuxAB Bacterial Bioluminescence. Chem Biol Drug Des 2016, 88(2):197-208.

 

2015  

26. Li S#, Wang J#, Li X, Yin S, Wang W*, Yang K*: Genome-wide identification and evaluation of constitutive promoters in streptomycetes. Microb Cell Fact 2015, 14(1):172.

27. Li S#, Wang W#, Li X, Fan K, Yang K*: Genome-wide identification and characterization of reference genes with different transcript abundances for Streptomyces coelicolor. Sci Rep 2015, 5:15840.

28. Li X#, Wang J, Li S, Ji J, Wang W*, Yang K*: ScbR- and ScbR2-mediated signal transduction networks coordinate complex physiological responses in Streptomyces coelicolor. Sci Rep 2015, 5:14831.

29. Wang W#, Li X#, Li Y, Li S, Fan K, Yang K*: A genetic biosensor for identification of transcriptional repressors of target promoters. Sci Rep 2015, 5:15887.

30. Yin S#, Wang W#, Wang X, Zhu Y, Jia X, Li S, Yuan F, Zhang Y*, Yang K*: Identification of a cluster-situated activator of oxytetracycline biosynthesis and manipulation of its expression for improved oxytetracycline production in Streptomyces rimosus. Microb Cell Fact 2015, 14(1):46.

 

2014  

31. Wang W#, Ji J#, Li X, Wang J, Li S, Pan G, Fan K, Yang K*: Angucyclines as signals modulate the behaviors of Streptomyces coelicolor. Proc Natl Acad Sci U S A 2014, 111(15):5688-5693.

 

2013  

32. Wang W#, Li X#, Wang J, Xiang S, Feng X, Yang K*: An engineered strong promoter for streptomycetes. Appl Environ Microbiol 2013, 79(14):4484-4492.

33. Liu Z#, Wang W, Zhu Y, Gong Q, Yu W*, Lu X*: Antibiotics at subinhibitory concentrations improve the quorum sensing behavior of Chromobacterium violaceum. FEMS Microbiol Lett 2013, 341(1):37-44.

 

2012  

34. Fan K#, Pan G#, Peng X, Zheng J, Gao W, Wang J, Wang W, Li Y, Yang K*: Identification of JadG as the B ring opening oxygenase catalyzing the oxidative C-C bond cleavage reaction in jadomycin biosynthesis. Chem Biol 2012, 19(11):1381-1390.

 

2011  

35. Wang J#, Wang W#, Wang L, Zhang G, Fan K, Tan H, Yang K*: A novel role of 'pseudo'gamma-butyrolactone receptors in controlling gamma-butyrolactone biosynthesis in Streptomyces. Mol Microbiol 2011, 82(1):236-250.

 

 

申请或授权专利

申请专利: 

1. 基于CRISPR/Cas12a系统的生物传感器、试剂盒及其在小分子检测中的用途;发明人:张立新 谭高翼 梁敏东 王为善 刘家坤;申请号:201910285607.1;申请日:2019.04.10 

2. 提高链霉菌聚酮化合物产量的方法;发明人:张立新 王为善 李珊珊 李子龙 谭高翼 范可强 张敬宇;申请号:201910411123.7;申请日:2019.05.17  

3. 包含别构转录因子调控系统的生物传感器、试剂盒及其在小分子检测中的用途。申请号:201810224843;申请日期:2018.06.06;发明人:张立新,王为善,姚永鹏,曹佳倩,李珊珊,李子龙 

4. 高温产核黄素的工程菌及其构建方法及应用。申请号:201810349923.6;申请日期:2018.04.18;发明人:李子龙,齐凤仙,范可强,王为善 

5. 包含别构转录因子调控系统的生物传感器、试剂盒及其在小分子检测中的用途。申请号:201610934491.6;申请日期:2016.10.25;发明人:杨克迁;王为善;李珊珊;范可强;李子龙 

6. 一种提高链霉菌次级代谢产物产量的方法。申请号:201710953975.X;申请日期:2017.10.13;发明人:李珊珊,向文胜,王为善,王俊阳 

7. 利用土霉素正调控基因构建重组菌和提高土霉素产量的方法;申请号:201510003536.3;申请日期:2015.01.04;发明人:杨克迁,王为善,尹守亮,王娟,王雪峰,贾晓乐,袁昉 

授权专利: 

1.  利用土霉素正调控基因构建重组菌和提高土霉素产量的方法;专利号:ZL201510003536.3;授权公告号:CN105821053B;授权日期:2019.09.20;发明人:杨克迁,王为善,尹守亮,王娟,王雪峰,贾晓乐,袁昉