代表性图片

　　A.

　　A. 铜绿假单胞菌生物被膜的发育周期及其相应阶段Psl多糖形成的基质网的变化。

　　红色：Psl多糖； 绿色：细菌细胞(Ma et. al. 2009 PLoS Pathogens)

　　B.

　　C.

　　B. 铜绿假单胞菌Biofilm中蜘蛛网形态的Psl多糖丝状网络 C. 四型菌毛介导的运动导致多糖丝状网络形成的模式图

　　红色：Psl多糖； 绿色：细菌细胞(Wang et. al., 2013 Environ Microbiol)

代表性论文

1.Zhao T, Lei F, Zhang Z, Wang D*, Ma LZ* (2025) Extracellular aminopeptidase regulates exopolysaccharide production of Pseudomonas aeruginosa via quorum sensing. ISME J. doi:101093/ismejo/wraf038.

2.Yu Z, Wu Z, Liu D, Liu H, Zhang Y, Zheng Y, Huang Y, Liao S, Wei Y, Huang W, Zhang Z, Liu X, Yu H, Wang D, Li L, Long F*, Ma LZ* (2025) Dual-function regulator MexL as a target to control phenazines production and pathogenesis of Pseudomonas aeruginosa. Nat Commun. 16(1):2000 doi:101038/s41467-025-57294-8.

3.Xie W, Gao X, Zheng M, Tan Z, Liu Y, Ma LZ*, Liu Z*. (2025) The novel Dirammox process for advanced nitrogen removal from high COD content and ammonium-rich wastewater. Environ Technol Inno. 37:103960.

4.Zhao M, Zhang Y, Wang M, Ma LZ* (2024) dsAMP and dsAMPGAN: Deep Learning Networks for Antimicrobial Peptides Recognition and Generation. Antibiotics (Basel).13(10)doi:103390/antibiotics13100948.

5.Shi H, Gong C, Zheng M, Zhao Y, Liu Y, Ma L*, Liu Z*. (2024) Arsenic Enhances the Degradation of Middle-Chain Petroleum Hydrocarbons by Rhodococcus sp. 2021 Under Their Combined Pollution. Microorganisms. 12(11):2279. 

6.Zhang H, Zhang W, Zong Y, Kong D, Ma L*, Wu X-L*, Zhao K*. (2024) Dynamics of microbial-induced oil degradation at the microscale. Microbiol Spectr. 12(12):e0117624.

7.Wang D#, Syed Tatheer Alam N#, Lei F#, Zhang Z, Yu H, Ma LZ.* (2023) Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads. Biofilm.16;6:100155.

8.Liu X, Jia M, Wang J, Cheng H, Cai Z, Yu Z, Liu Y, Ma LZ, Zhang L, Zhang Y, Yang L* (2023) Cell division factor ZapE regulates Pseudomonas aeruginosa biofilm formation by impacting the pqs quorum sensing system. mLife. 21;2(1):28-42.

9.Liu D, Wang D, Wei Q, Zhang Y, Yu H, Ma LZ* (2023) A Library of Promoter-gfp Fusion Reporters for Studying Systematic Expression Pattern of Cyclic-di-GMP Metabolism-Related Genes in Pseudomonas aeruginosa. Appl Environ Microbiol. 28;89(2):e0189122.

10.Zhang Y#, Pramod B#, Dinesh SR#, Liu D#, Yu Z#, Zhao T, Zheng Y, Amit K, Yu H, Ma LZ* (2023) Dual functions: A coumarin–chalcone conjugate inhibits cyclic-di-GMP and quorum-sensing signaling to reduce biofilm formation and virulence of pathogens. mLife. 10:1022.

11.Zhang Y#, Zhou C#, Zhou Y#, Zheng Y#, Yu Y, Yang K, Chen Z, Chen X, Wen K, Chen Y, Bai S, Song J, Wu T, Lei E, Wan M, Cai Q*, Ma L*, Wong WL*, Bai Y*, Zhang C*, Feng X* (2023)Amphiphilic Nano-Swords for Direct Penetration and Eradication of Pathogenic Bacterial Biofilms. ACS Appl Mater Interfaces. 15(16):20458-20473.

12.Ma LZ, Wang D, Liu Y, Zhang Z, Wozniak D. (2022) Regulation of Biofilm Exopolysaccharide Biosynthesis and Degradation in Pseudomonas aeruginosa. Annu Rev Microbiol. 76:413-433 doi: 101146/annurev-micro-041320-111355. 

13.Zhang J#, Wu H#, Wang D#, Wang L, Cui Y, Zhang C, Zhao K*, Ma LZ* (2022) Intracellular glycosyl hydrolase PslG shapes bacterial cell fate, signaling, and the biofilm development of Pseudomonas aeruginosa. eLife.11:e72778 doi: 107554/eLife72778.

14.Xu A, Wang D, Wang Y, Zhang L, Xie Z, Cui Y, Bhamse P, Yu H, Zhang XX, Li D, Ma LZ* (2022)Mutations in surface-sensing receptor WspA lock the Wsp signal transduction system into a constitutively active state. Environ Microbiol. 24(3):1150-1165 doi: 101111/1462-292015763.

15.Zhang Y, Cai Y, Zeng L, Liu P, Ma LZ, Liu J. (2022) A microfluidic approach for quantitative study of spatial heterogeneity in bacterial biofilms. Small Sci. 2200047.

16.Xu F, Liu S, Naren N, Li L, Ma LZ, Zhang XX. (2022) Experimental evolution of bacterial survival on metallic copper. Ecol Evol. 12: e9225.

17.Zhang Y, Huang HH, Ma LZ, Masuda Y, Honjoh KI, Miyamoto T. (2022) Inactivation of mixed Escherichia coli O157:H7 biofilms on lettuce by bacteriophage in combination with slightly acidic hypochlorous water (SAHW) and mild heat treatment. Food Microbiol. 104:104010 doi: 101016/jfm2022104010.

18.Zheng Y, Wang D, Ma LZ. (2021) Effect of Polyhexamethylene Biguanide in Combination with Undecylenamidopropyl Betaine or PslG on Biofilm Clearance. Int J Mol Sci. 22(2):768 doi:103390/ijms22020768.

19.Zhang Y, Cai Y, Zeng L, Liu P, Ma LZ, Liu J. (2022) A Microfluidic Approach for Quantitative Study of Spatial Heterogeneity in Bacterial Biofilms. Small Sci. 2(10):2200047. 

20.Xu A#, Wang D #, Ding Y, Zheng Y, Wang B, Wei Q, Wang S, Yang L and Ma LZ* (2020) Integrated comparative genomic analysis and phenotypic profiling of Pseudomonas aeruginosa isolates from crude oil. Front Microbiol. 11:519.

21.Xu A, Zhang M, Du W, Wang D^* and Ma LZ^* (2020) A molecular mechanism for how sigma factor AlgT and transcriptional regulator AmrZ inhibit twitching motility in Pseudomonas aeruginosa. Environ Microbiol. doi:101111/1462-292014985.

22.Wei Q, Bhasme P, Wang Z, Wang L, Wang S, Zeng Y, Wang Y, Ma LZ ^*and Li Y^* (2020) Chinese medicinal herb extract inhibits PQS-mediated quorum sensing system in Pseudomonas aeruginosa. J Ethnopharmacol. 248, 112272.

23.Bhasme P, Wei Q, Xu A, Naqvi S, Wang D and Ma LZ ^* (2020) Evaluation and characterization of the predicted diguanylate cyclase-encoding genes in Pseudomonas aeruginosa. MicrobiologyOpen, 9(3): e975.

24.Zhao T, Zhang J, Tang M, Ma LZ^* and Lei X.^* (2019) Development of an effective fluorescence probe for discovery of aminopeptidase inhibitors to suppress biofilm formation. J Antibiot, 72, 461-468.

25.Wu H, Wang D, Tang M, and Ma LZ. ^* (2019) The advance of assembly of exopolysaccharide Psl biosynthesis machinery in Pseudomonas aeruginosa. MicrobiologyOpen, 8(10): e857.

26.Wu H, Qiao S, Li D, Guo L, Zhu M and Ma LZ ^* (2019) Crystal structure of the glycoside hydrolase PssZ from Listeria monocytogenes. Acta Crystallogr F, Structural Biology Communications, 75, 501-506.

27.Wei Q, Leclercq S, Bhasme P, Xu A, Zhu B, Zhang Y, Zhang M, Wang S and Ma LZ^* (2019) Diguanylate Cyclases and Phosphodiesterases Required for Basal-Level c-di-GMP in Pseudomonas aeruginosa as Revealed by Systematic Phylogenetic and Transcriptomic Analyses. Appl Environ Microbiol, 16;85(21) pii: e01194-19.

28.Liu X, Wang S, Xu A, Zhang L, Liu H and Ma LZ ^* (2019) Biological synthesis of high-conductive pili in aerobic bacterium Pseudomonas aeruginosa. Appl Microbiol Biotechnol, 103, 1535-1544.

29.Huang Z, Wang Y, Zhu H, Andrianova EP, Jiang, C, Li D, Ma LZ, Feng J, Liu ZP, Xiang H^* (2019) Cross Talk between Chemosensory Pathways That Modulate Chemotaxis and Biofilm Formation. mBio, 10(1) pii: e02876-18.

30.Zhao, T, Zhang, Y, Wu, H, Wang, D, Chen, Y, Zhu, MJ and Ma, LZ^* (2018) Extracellular aminopeptidase modulates biofilm development of Pseudomonas aeruginosa by affecting matrix exopolysaccharide and bacterial cell death. Environ Microbiol Rep, 10, 583-593.

31.Zhang J, He J, Zhai C, Ma LZ, Gu L and Zhao K^* (2018) Effects of PslG on the Surface Movement of Pseudomonas aeruginosa. Appl Environ Microbiol, 84.

32.Zhai C, Zhang W, Zhang J, Ma LZ and Zhao K ^* (2018) Overshadow Effect of Psl on Bacterial Response to Physiochemically Distinct Surfaces Through Motility-Based Characterization. Front Cell Infect Mi, 8, 383.

33.Jin, Z, Nie, M, Hu, R, Zhao, T, Xu, J, Chen, D, Yun, J, Ma, LZ and Du, W ^* (2018) Dynamic Sessile-Droplet Habitats for Controllable Cultivation of Bacterial Biofilm. Small (Weinheim an der Bergstrasse, Germany), 14, e1800658.

34.Yu S, Zhang M and Ma L^* (2017) Anti-biofilm effects of Zn lactate3H(2)O and SnF(2) on Pseudomonas aeruginosa, Acinetobacter baumannii and Streptococcus mutans. Sheng wu gong cheng xue bao = Chinese Journal of Biotechnology, 33, 1478-1488.

35.Yu S and Ma L ^* (2017) Iron uptake and biofilm formation in Pseudomonas aeruginosa. Sheng wu gong cheng xue bao = Chinese Journal of Biotechnology, 33, 1489-1512.

36.Wang D, Xu A, Elmerich C and Ma LZ^* (2017) Biofilm formation enables free-living nitrogen-fixing rhizobacteria to fix nitrogen under aerobic conditions. ISME J, 11, 1602-1613.

37.Qian W, Ma L, Gu L and Zhang L (2017) Preface for special issue on biofilm and c-di-GMP--Microbial society, c-di-GMP regulation, and new research techniques. Sheng wu gong cheng xue bao = Chinese Journal of Biotechnology, 33, 1351-1356.

38.Rahman MRT, Ma LZ, Rahman MS.* (2017) Use of pH (acids and alkali) as Disinfection Methods. In the sixth edition of Block’s Disinfection, Sterilization, and Preservation. Gerald McDonnell and Joyce Hansen (eds), Wolters Kluwer Health Inc.

39.Zhu B, Liu C, Liu S, Cong H, Chen Y, Gu L and Ma LZ^* (2016) Membrane association of SadC enhances its diguanylate cyclase activity to control exopolysaccharides synthesis and biofilm formation in Pseudomonas aeruginosa. Environ Microbiol, 18, 3440-3452.

40.Yu S, Wei Q, Zhao T, Guo Y and Ma LZ^* (2016) A Survival Strategy for Pseudomonas aeruginosa That Uses Exopolysaccharides To Sequester and Store Iron To Stimulate Psl-Dependent Biofilm Formation. Appl Environ Microbiol, 82, 6403-6413.

41.Yu S ^#, Su T ^#, Wu H, Liu S, Wang D, Zhao T, Jin Z, Du W, Zhu MJ, Chua SL Gu L^*,Ma, LZ^* (2015) PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix. Cell Res, 25, 1352-1367.

42.Wang S, Liu X, Liu H, Zhang L, Guo Y, Yu S, Wozniak DJ and Ma LZ^* (2015) The exopolysaccharide Psl-eDNA interaction enables the formation of a biofilm skeleton in Pseudomonas aeruginosa. Environ Microbiol Rep, 7, 330-340.

43.Wang D, Hildebrand F, Ye L, Wei Q and Ma LZ^* (2015) Genome Sequence of Mucoid Pseudomonas aeruginosa Strain FRD1. Genome Announ, 3.

44.Wang S, Yu S, Zhang Z, Wei Q, Yan L, Ai G, Liu H and Ma LZ^* (2014) Coordination of swarming motility, biosurfactant synthesis, and biofilm matrix exopolysaccharide production in Pseudomonas aeruginosa. Appl Environ Microbiol, 80, 6724-6732.

45.Das P, Yang XP and Ma LZ^* (2014) Analysis of biosurfactants from industrially viable Pseudomonas strain isolated from crude oil suggests how rhamnolipids congeners affect emulsification property and antimicrobial activity. Front Microbiol, 5, 696.

46.Wei Q and Ma LZ^* (2013) Biofilm matrix and its regulation in Pseudomonas aeruginosa. Int J Mol Sci, 14, 20983-21005.

47.Wang S, Parsek MR, Wozniak DJ and Ma LZ^* (2013) A spider web strategy of type IV pili-mediated migration to build a fibre-like Psl polysaccharide matrix in Pseudomonas aeruginosa biofilms. Environ Microbiol, 15, 2238-2253.

48.Ma LZ^*, Wang S, Wang D, Parsek MR and Wozniak DJ. (2012) The roles of biofilm matrix polysaccharide Psl in mucoid Pseudomonas aeruginosa biofilms FEMS Immuno Med Mic, 65, 377-380

49.Ma LZ^*, Wang J, Wang S, Anderson EM, Lam JS, Parsek MR and Wozniak DJ (2012) Synthesis of multiple Pseudomonas aeruginosa biofilm matrix exopolysaccharides is post-transcriptionally regulated. Environ Microbiol, 14, 1995-2005.

50.Ma L, Liu X, Liang H, Che Y, Chen C, Dai H, Yu K, Liu M, Ma L, Yang CH, Song F, Wang Y, Zhang L. ^* (2012) Effects of 14-alpha-lipoyl andrographolide on quorum sensing in Pseudomonas aeruginosa. Antimicrob Agents Ch, 56, 6088-6094.

51.Starkey M, Hickman JH, Ma L, Zhang N, De Long S, Hinz A, Palacios S, Manoil C, Kirisits MJ, Starner TD. (2009) Pseudomonas aeruginosa rugose small-colony variants have adaptations that likely promote persistence in the cystic fibrosis lung. J Bacteriol, 191, 3492-3503.

52.Ma L, Conover M, Lu H, Parsek MR, Bayles K and Wozniak DJ. ^* (2009) Assembly and development of the Pseudomonas aeruginosa biofilm matrix. PLoS Pathog, 5, e1000354.

53.Byrd MS, Sadovskaya I, Vinogradov E, Lu H, Sprinkle AB, Richardson SH, Ma L, Ralston B, Parsek MR, Anderson EM (2009) Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production. Mol Microbiol, 73, 622-638.

54.Huigens RW 3rd, Ma L, Gambino C, Moeller PD, Basso A, Cavanagh J, Wozniak DJ and Melander, C (2008) Control of bacterial biofilms with marine alkaloid derivatives. Mol Biosyst, 4, 614-621.

55.Ma L, Lu H, Sprinkle A, Parsek MR and Wozniak DJ. ^* (2007) Pseudomonas aeruginosa Psl is a galactose- and mannose-rich exopolysaccharide Journal of Bacteriology, 189, 8353-8356.

56.Taghbalout A, Ma L and Rothfield L. ^* (2006) Role of MinD-membrane association in Min protein interactions. J Bacteriol, 188, 2993-3001.

57.Ma L, Jackson KD, Landry RM, Parsek MR and Wozniak DJ.^* (2006) Analysis of Pseudomonas aeruginosa conditional psl variants reveals roles for the psl polysaccharide in adhesion and maintaining biofilm structure postattachment. J Bacteriol, 188, 8213-8221.

58.Cui Y, Tu R, Guan Y, Ma L and Chen S.^* (2006) Cloning, sequencing, and characterization of the Azospirillum brasilense fhuE gene. Cur Microbiol, 52, 169-177.

59.Schimanski B, Ma L and Gunzl A .^* (2004) Failure to detect binding of Trypanosoma brucei SNAPc to U2 and U6 snRNA gene sequences by in vitro transcription competition and pull-down assays. Mol Biochem Parasit, 137, 293-296.

60.Ma L, King GF and Rothfield L.^* (2004) Positioning of the MinE binding site on the MinD surface suggests a plausible mechanism for activation of the Escherichia coli MinD ATPase during division site selection. Mol Microbiol, 54, 99-108.

61.Desnoues N, Lin M, Guo X, Ma L, Carreno-Lopez R and Elmerich C.^* (2003) Nitrogen fixation genetics and regulation in a Pseudomonas stutzeri strain associated with rice. Microbiology (Reading, England), 149, 2251-2262.

62.Wang J, Chen S-F, Ma L-Y, Li J-L.^* (2001) Cloning, sequencing and expression pattern, functional analysis of nifA in Azospirillum brasilense Yu62. Acta Microbiologia Sinica 41:655-661(in Chinese ).

63.Ma L-Y, Wu Y Wang J, Zhao Y-S, Li J-L.^* (1999) Site-directed mutagenesis analysis of draTG genes and their downstream region from Azospirillum brasilense Yu62. Chinese J of Biotechnology 15:281-287 (in Chinese).

64.Ma L-Y and Li J-L. ^* (1997) Advance in genetics of Azospirillum and plant-root interactions (a review).  High Technology letters 11:56-59. (in Chinese).

65.Ma L-Y and Li J-L. ^* (1997) Sequencing and analysis of function of the upstream region of draTG genes from Azospirillum brasilense Yu62. Chinese J of Biotechnology 13(4):343-349 (in Chinese and English).

66.Ma L-Y and Li J-L.^* (1997) Cloning and sequencing of draTG genes and downstream region from Azospirillum brasilense Yu62. Chinese J of Biotechnology 13(3):227-235 (in Chinese and English).

67.Yan D-L, He L-H, Ma L-Y, Li J-L.^* (1995) Effect of Klebsiella pneumoniae nifA on the regulation of nif gene expression by ammonia in Azospirillum brasilense. Chinese J of Biotechnology 11(4):385-388 (in Chinese).

68.Yan D-L, He L-H, Ma L-Y, Li J-L.^* (1995) Transcriptional activation of Azospirillum brasilense nifH promoter by NifA of Klebsiella pneumoniae. Chinese J of Biotechnology 11(3):205-210 (in Chinese).

69.Ma L-Y, Yan D-L, He L-H, Li J-L.^* (1994) Insertion mutagensis used in nitrogen-fixing bacteria (a review). Microbiology 21:101-105 (in Chinese).

70.Nan Q-X, Ren F-ZH, Yang Z-B, Zhang X-J, Ma L-Y (1992) Studies on the forming process of milk fan in Yun nan province. Chinese J of Animal Science 28(2):25-27.

 申请及授权专利

1.马旅雁,郑雅倩,于海英,匡昕雯,王迪，抑菌相关的蛋白及其生物材料和应用。专利申请号：202411633860.9，申请日：2024-11-15。

2.马旅雁,张瑜,刘德健,于海英,王迪，肽聚糖水解酶及其生物材料和应用。专利申请号：202411275708.8，申请日：2024-09-12。

3.马旅雁,于召箫,张瑜,刘德健,郑雅倩,于海英,王迪，用于治疗细菌感染的药物组合物。专利申请号：202411253300.0，申请日：2024-09-09。

4.马旅雁，于召箫，于海英，王迪，MexL 蛋白在调控铜绿假单胞菌毒性中的应用。专利申请号：CNCN202310675915.1，申请日：2023-06-08。

5.马旅雁,于海英，赵敏，郑雅倩，王迪，不同比例单双鼠李糖脂生产菌株的构建及其应用。专利申请号：CN202310365019.5， 申请日：2023-04-07。

6.马旅雁，郑雅倩，王迪，于海英，PslG蛋白在隐形眼镜护理液中的应用。中国发明专利号：ZL202111138724.9，公告日：2023-3-21。

7.Luyan Ma, Qing Wei, Pramod Bhasme, Di Wang, Methoda and compositions for inhibiting formation of biofilms. 美国专利申请，专利申请号：17454626，2023-12-13。

8.MA Lvyan, YU Shan, WU Huijun, WANG Shiwei, Wang Di, Reagent and methods for inhibiting or disrupting biofilm Patent No: US10499655B2 (20191210).

9.马旅雁，王世伟，王迪，杨新平。高效抑菌鼠李糖脂高产菌株及其应用。授权号：CN103232957A。

10.马旅雁，王世伟，王迪，杨新平。一株适用于油藏环境的假单胞菌及其应用。授权号: CN103215207A。

11.马旅雁，王迪，张妙坤，赵天湖，王世伟。PslG蛋白或其编码序列在检测微生物数量方面的应用和方法。专利号CN108660123B。申请日期 2018-2-22，授权日期2020-10-2。

12.马旅雁、刘茜、王世伟。导电四型菌毛及其编码基因和含有该基因的载体和相应的生产菌株及其应用。申请号：2018105557946

13.马旅雁、王迪、张妙坤、赵天湖、王世伟PslG蛋白或其编码序列在检测微生物数量方面的应用和方法。申请号：2018101543268

14.马旅雁、王迪、王世伟、徐安明。一种促进固氮细菌形成胞囊结构的方法及其专用培养基。申请号：2017100340312。

15.马旅雁、谷立川、于珊、吴慧君、王世伟、苏甜甜、王迪、杨亮。用于抑制/瓦解生物被膜的抑制剂及其应用。申请号：PCT/CN2016/076171。

16.马旅雁、谷立川、于珊、吴慧君、王世伟、苏甜甜、王迪、杨亮。用于抑制/瓦解生物被膜的抑制剂及其应用。申请号： 2015101127466。(2020已授权)

17.马旅雁，王世伟。提高鼠李糖脂产量的方法及其专用铜绿假单胞菌。申请号： 201410328471。

 参编专著

1.马旅雁，王世伟，张真印，杨霁舟，（2012）生物膜形成的机制， 《微生物生物膜与感染》周学东、施文元主编，人民卫生出版社， p1-34.

2.马旅雁，李季伦*，（1998）固氮遗传学研究进展（综述），《农业生物工程》莽克强主编，陈受宜、李季伦、朱裕鼎副主编，化学工业出版社，p15-48.