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Laboratory of Bacterial Physiology and Metabolism Principal Investigator
Research Area: Research Interests:
Lab Members Principal Investigator
Education Experience 1987/1993 Ph.D., Under Professor Leo C. Vining Professional Experience 2003/present Professor
Staff
Postdoctoral Fellow
Graduate Students
Former Graduate Students Hongbo Mo 2000-2003
Recent Publications: [1] Dekun Guo, Youbao Zhao,Keqian Yang*. Coordination of Glycerol Utilization and Clavulanic Acid Biosynthesis to Improve Clavulanic Acid Production in Streptomyces Clavuligerus.Science China Life Sciences,2013, 56(7):591–600. [2] Pauli Kallio, Pekka Patrikainen, Georgiy A. Belogurov, Pekka M？nts？l？, Keqian Yang, Jarmo Niemi,Mikko Mets？-Ketel？*.Tracing the Evolution of Angucyclinone Monooxygenases: Structural Determinants for C-12b Hydroxylation and Substrate Inhibition in PgaE.Biochemistry,2013, 52(26):4507-4516. [3] Weishan Wang, Xiao Li, Juan Wang, Sihai Xiang, Xiaozhou Feng,Keqian Yang*.An Engineered Strong Promoter for Streptomycetes.Applied and Environmental Microbiology,2013, 79(14):4484-4492. [4] Youbao Zhao, Sihai Xiang, Xida Dai,Keqian Yang*.A simplified diphenylamine colorimetric method for growth quantification.AppliedMicrobiology andBiotechnology, 2013, 97(11):5069-5077. [5] Xida Dai, Sihai Xiang, Jia Li,Qiang Gao*,Keqian Yang*. Development of a colorimetric assay for rapid quantitative measurement of clavulanic acid in microbial samples.Science China Life Sciences,2012, 55(2):158–163. [6] Keqiang Fan, Guohui Pan, Xiaojing Peng, Jianting Zheng, Wubin Gao, Juan Wang, Weishan Wang, Yue Li,Keqian Yang*. Identification of JadG as the B Ring Opening Oxygenase Catalyzing the Oxidative C-C Bond Cleavage Reaction in Jadomycin Biosynthesis.Chemistry＆Biology,2012, 19(11):1381–1390. [7] Keqiang Fan, Xia Zhang, Hongchun Liu, Hui Han, Yuanming Luo, Qian Wang, Meiyu Geng,Keqian Yang*. Evaluation of the cytotoxic activity of new jadomycin derivatives reveals the potential to improve its selectivity against tumor cells.The Journal of Antibiotics,2012, 65(9):449–452. [8] Junjie Ji, Keqiang Fan, Xiuyun Tian, Xia Zhang, YuxiuZhang,Keqian Yang*. Iterative Combinatorial Mutagenesis as an Effective Strategy for Generation of Deacetoxycephalosporin C Synthase with Improved Activity toward Penicillin G.Applied and Environmental Microbiology, 2012, 78(21):7809–7812. [10] Pekka Patrikainen, Pauli Kallio, Keqiang Fan, Karel D. Klika, Khaled A. Shaaban, Pekka Mantsala, Jurgen Rohr, Keqian Yang, Jarmo Niemi,Mikko Metsa-Ketela*. Tailoring Enzymes Involved in the Biosynthesis of Angucyclines Contain Latent Context-Dependent Catalytic Activities.Chemistry＆Biology,2012,19(5):647–655. [11] Hua Yin, Sihai Xiang, Jianting Zheng, Keqiang Fan, TingtingYu, Xu Yang, Yanfeng Peng, Haibin Wang, Deqin Feng, Yuanming Luo, Hua Bai,Keqian Yang*. Induction of Holomycin Production andComplex Metabolic Changes by theargRMutation in Streptomyces clavuligerus NP1.Applied and Environmental Microbiology,2012, 78(9):3431–3441. [12] Tingting Yu, Xinping Xu, Yanfeng Peng, Yuanming Luo,Keqian Yang*. Cell wall proteome of Clostridium thermocellum and detection of glycoproteins.Microbiological Research,2012,167(6):364–371. [13] Yongchang Ouyang, Houbo Wu, Lianwu Xie, Guanghua Wang, Shikun Dai, Minjie Chen,Keqian Yang*, Xiang Li. A method to type the potential angucycline producers in actinomycetes isolated from marine sponges.Antonie Van Leeuwenhoek, 2011, 99(4):807–815. [14] Yanfeng Peng, Yuanming Luo, Tingting Yu, Xinping Xu, Keqiang Fan, Youbao Zhao,Keqian Yang*. A blue native-PAGE analysis of membrane protein complexes in Clostridium thermocellum.BMC Microbiology, 2011, 11(1):22. [15] Juan Wang, Weishan Wang, Linqi Wang, Guifeng Zhang, Keqiang Fan, Huarong Tan,Keqian Yang*. A novel role of “pseudo” gamma-butyrolactone receptors in controlling gamma-butyrolactone biosynthesis in Streptomyces.Molecular Microbiology, 2011, 82(1):236–250. [16] Xiaobin Wu, Xiuyun Tian, Junjie Ji, Weibin Wu, Keqiang Fan,Keqian Yang*. Saturation mutagenesis of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase R308 site confirms its role in controlling substrate specificity.Biotechnology Letters, 2011, 33(4):805–812. [17] Tingting Yu, Yuanming Luo,Keqian Yang*. Rapid characterization of N-linked glycosylation site using non-specific protease digestion of gel-separated glycoproteins and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry.European Journal of Mass Spectrometry (Chichester, England), 2011, 17(6):573–579. [18] Yihua Chen, Keqiang Fan, Yongzhi He, Xinping Xu, Yanfeng Peng, Tingting Yu, Cuijuan Jia,Keqian Yang*. Characterization of JadH as an FAD- and NAD(P)H-dependent bifunctional hydroxylase/dehydrase in jadomycin biosynthesis.ChemBioChem, 2010, 11(8):1055–1060. [19] Gangming Xu, Juan Wang, Linqi Wang, Xiuyun Tian, Haihua Yang, Keqiang Fan,Keqian Yang*, Huarong Tan. “Pseudo” gamma-butyrolactone receptors respond to antibiotic signals to coordinate antibiotic biosynthesis.The Journal of Biological Chemistry, 2010, 285(35):27440–27448. [20] Linqi Wang, Xiuyun Tian, Juan Wang, Haihua Yang, Keqiang Fan, Gangming Xu,Keqian Yang*, Huarong Tan. Autoregulation of antibiotic biosynthesis by binding of the end product to an atypical response regulator.Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(21):8617–8622. [21] Sihai Xiang, Jia Li, Hua Yin, Jianting Zheng, Xu Yang, Haibin Wang, Jiali Luo, Hua Bai,Keqian Yang*. Application of a double-reporter-guided mutant selection method to improve clavulanic acid production in Streptomyces clavuligerus.Metabolic Engineering, 2009, 11(4-5):310–318.
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Keqian Yang, Ph. D., Professor
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