Zhiyang DONG

Molecular Enzymology Research Group

               Principal Investigator
           Prof. Dr.Dong Zhiyang

             Tel：010-64807337
        E-mail: dongzy@sun.im.ac.cn

Research:

The research works of this group is focused on some industrial enzyme from various microorganisms. Molecular technology had been used to get some enzyme gene from metagenomic liberary of extreme environment. Some importment enzyme gene had been found and been over-expressed for industrial application .

Key words:Xylanase,Cellulase,Metagenomic liberary

Background and Significance:
1) Cellulose-degrading enzymes research for biofuel production and feed.
2）Construction of metagenomic library from extreme environments and Screening for industrial enzyme genes.
3) high-efficient enzyme gene secretion expression system construction
4) Higher industrial enzyme productive engineering strain construction and
5) Study on the function and structure of archaeal chaperonin from Acidianus Tengchongeneses sp.S5

Major Achievements：

In the past year, the research works of this group has been supported by the fund of NSFC, “973” project from MOST, China , “863” project from MOST, China. Some important industry enzymes such as Xylanase，cellulase had been producted with over-expression recombinant strain and applied in feed, paper industry. A series of extreme environment metagenomic library including a rumen metagenomic BAC library had been constructed, a series of new gene of enzyme had been found in those library with HTS. A thermostability new enzyme gene had been screened by HTS. Some new archaeal chaperonin had been found in Acidianus Tengchongeneses sp.S5.A pilot plant for industial enzyme had been set up for production of xylanase, cellulase. Some enzymes had been applied successful in pulp manufacture and fuel enthanol production.

Group Members:

Principal Investigator

Prof. Dr.Dong Zhiyang

Education:

1993-1995INSTITUTE OF MICROBIOLOGY, Department of Enzymology, Chinese Academy of Sciences ,Beijing,CHINA
.

• Postdoctoral in Enzymology and Glycoprotein
• Advisor :Prof.Zhang shuzheng
• Dissertation Title: Structure and Function of Glycoprotein- effecting
  of N-linked Oligosaccharide on the conformation and properties of
  Glucoamylase From Monascus rubiginosus.

1990-1993BEIJING AGRICULTURE UNIVERSITY, Department of plant biochemistry

• Ph.D in the Biology of Contractile protein.
• Advisor:Prof.Long-Fei Yan
• Dissertation title: Structure and Function of contractile protein from
  Physarum plasmodium

1985-1988BEIJING AGRICULTURE UNIVERSITY, Department of plant biochemistry

• Graduate
• Thesis Title: Immobilization of Papain

1981-1985SICHUAN UNIVERSITY Department of Biology,Chengdu,Sichuan,China

• Bachelor of Science in Biochemistry
• Advisor:Prof.Fan Kaiming
• Graduation Thesis Title: Study on blood Factor VIII

Professional Experience:

2/96-presentChinese Academy of Sciences, Institute of Microbiology, Department of Enzymology

• Associate Professor
• Studying chitinase and chitooligosaccharide preparation
• The highlight of research: Research on microbial enzyme gene cloning and expression, such as xylanase, cellulase, SOD, ？-glucosiase, ？-glucanase et.
• Metagenomic library construction from some special environment and enzyme gene screening from the library.
• Some importance industrial enzyme directed molecular evolution.

9/93-2/96Chinese Academy of Sciences, Institute of Microbiology Department of Enzymology

• Postdoctoral
• Studying the structure and function of the glycoprotien
• The highlight of research: Glucoamylase from Monascus rubiginosus had been found to exist in multiple glycoforms, the different glycosylation had induced different conformation of the protein, when the N-linked oligosaccharide of the protein had been removed by endo-H, the conformation of the protein had varied obviously.
• 18th AMBO international Training Course,Tokyo, Japan,1995.

9/90-7/93Beijing Agriculture University, Department of Plant Biochemistry

• Study on non-muscle contractile protein of Physarum plasmodium
• The highlight of research: I investigated some contractile proteins in Physarum plasmodium including actin、myosin and kinesin. This research proved that kinesin existed in Physarum plasmodium with western blot and the result showed that actin、myosin had play important role in the movement of Physarum plasmodium .

Honors:

• Fellowship from the Graduate School of Beijing Agriculture University
• Fellowship from Asia Molecular Biology Organism
• Scholarship from Sichun University

Funding/Awards:

• S&T Progress Awards (second class) from Chinese high education commission (1997)
• Research grant from Chinese National Nature Science Foundation(2003)
• Research grant from Chinese National Programs for high Technology Research and Development (2003)

Membership in Professional Societies:

• Chinese Society for Biochemistry
• Chinese Society for Microbiology
• Chinese Society for Enzyme engineer

Assistant Researcher

Xiuzhen Chen, Ph.D.
Yongzhi He, Ms.

Ph.D. Students

Su Xiaoyun 2005~2009
Zhang Shan 2006~2010
Wang Sha 2004~2009
Wang Li 2004~2010

M.S. Students

Chu Xin 2005~2009
Cheng Fei 2006~2010
Sun Pan 2007~2010

Representitive Picture

Rumen Metagenomic BAC Library

Thermophilic Archaeon S5 Chaperonin

Recent Publication

1. 黑曲霉木聚糖酶在工业酒精酵母中的分泌表达 微生物学报 2005，45（1）135－138
2. Prokaryotes Diversity in the Rumen of Yak (Bos grunniens) and Jinnan Cattle (Bos taurus) estimated by 16S rDNA homology analyses. Anaerobe 11 207-215, 2005
3. Isolation and identification of one bacterial strain from a 3900 meter deep subsurface rocks Acta Pertrologica Sinica 2005 21(2) 540-544
4. Analysis of Bacterial Community in Donghai Terrestrial Deep Subsurface Acta Pertrologica Sinica 2005 21(2) 533-539
5. 云南腾冲热泉土壤微生物基因组文库的构建与分析 微生物学报 2006 46（3）427－431
6. Quantitative analysis of two uncultured bacterial species from the terrestrial deep subsurface by real-time PCR Acta Pertrologica Sinica 2006 22(9)2103-2106 IF=1.10
7. Molecular methods in microbiology study of rock-Microbial research in Chinese continental Acta Pertrologica Sinica. 2006 22(9) 2107-2111
8. 荷斯坦奶牛瘤胃微生物元基因组BAC文库的构建与分析 微生物学报 2007 47（2）：213～216
9. 瘤胃元基因组BAC文库研究 中国农业科学导报2007 9（5）53～57
10. Characterization of a hyperthermostable Fe-superoxide dismutase from hot spring
    Applied Microbiology and Biotechnology 2007 75：367－376
11. Sha Wang, Wei-Feng Liu, Yong-Zhi He, Ao Zhang, Li Huang, Zhiyang Dong*, Yong-Bin Yan*, 2008,Multistate folding of a hyperthermostable Fe-superoxide dismutase (TcSOD) in guanidinium hydrochloride: The importance of the quaternary structure.Biochimica et Biophysica Acta-Proteins and Proteomics1784:445–454   PDF(IF 3.078)
12. Xiaoyun Su, Shan Zhang, Li Wang, Zhiyang Dong*. 2009. Overexpression of IbpB enhances production of soluble active Streptomyces olivaceovirdis XynB in Escherichia coli.Biochemical and Biophysical Research Communications390(3): 673-677 (IF 2.648)
13. Sha Wang , YongBin Yan* and Zhiyang Dong*. 2009. Contributions of the C-Terminal Helix to the Structural stability of a Hyperthermophilic Fe-Superoxide Dismutase (TcSOD).International Journal of Molecular Sciences10(12): 5498-5512. (IF 0.978)
14. Xiaoyun Su, Xin Chu, Zhiyang Dong*. 2009. Identification of elevated transcripts in a Trichoderma reesei strain expressing a chimeric transcription activator using suppression subtractive hybridization.World Journal of Microbiology & Biotechnology25: 1075-1084. (IF 0.945)
15. Li Wang, Zhongjun Hu, Yuanming Luo, Yanwu Huo, Qing Ma, Yongzhi He, Yuying Zhang, Fei Sun*, Zhiyang Dong*. 2010. Distinct symmetry and limited peptide refolding activity of the thermosomes from the acidothermophilic archaeaAcidianus tengchongensisS5^T.Biochemical and Biophysical Research Communications393(2):228-234.PDF