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论文题目: Flavobacterium orientale sp nov., isolated from lake water
作者: Li Ai-Hua , Liu Hong-Can , and Zhou Yu-Guang*.
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刊物名称: International Journal of Systematic and Evolutionary Microbiology
期:
卷: 67
页: 108-112
年份: 2017
影响因子: 2.782
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摘要: Two Gram-stain-negative, obligately aerobic, non-motile, rod-shaped bacterial strains, designated SP3(T) and SP38, were isolated from a cold-water lake in the west of China. A polyphasic taxonomic study was performed for the strains. Alignment of 16S rRNA gene sequences indicated that strains SP3(T) and SP38 were associated with the genus Flavobacterium and were most closely related to Flavobacterium lacus NP180(T) (96.4% sequence similarity), Flavobacterium ponti GSW-R14(T) (95.6 %) and Flavobacterium yanchengense hg(T) (95.3 %). The genomic DNA G+C contents of strains SP3(T) and SP38 were 34.9 and 34.6 mol%, respectively. The major fatty acids were iso-C-15 : 0, iso-C-15 : 1 G, summed feature 9 (iso-C17 : 1 omega 9c and/or 10-methyl C-16 : 0), iso-C-17 : 0 3-OH and iso-C-15 : 0 3-OH. The unique respiratory quinone was menaquinone 6 (MK-6). The polar lipid profile consisted of phosphatidylethanolamine, one unidentified aminolipid and several unidentified polar lipids. Based on physiological, biochemical and phylogenetic data for these isolates, it was confirmed that strains SP3(T) and SP38 were affiliated to the genus Flavobacterium and represented a novel species, for which the name Flavobacterium orientale sp. nov. is proposed. The type strain is SP3(T) (= CGMCC 1.12506(T) = NBRC 109717(T)).