Anti-MRSA and anti-TB metabolites from marine-derived Verrucosispora sp MS100047----中国科学院微生物研究所

科研成果

论文题目:	Anti-MRSA and anti-TB metabolites from marine-derived Verrucosispora sp MS100047
作者:	Huang, Pei Xie, Feng Ren, Biao Wang, Qian Wang, Jian Wang, Qi Abdel-Mageed, Wael M. Liu, Miaomiao Han, Jianying Oyeleye, Ayokunmi Shen, Jinzhao Song, Fuhang Dai, Huanqin Liu, Xueting Zhang, Lixin
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刊物名称:	Applied Microbiology and Biotechnology
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年份:	2016
影响因子:	3.882
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摘要:	Microbes belonging to the genus Verrucosispora possess significant chemical diversity and biological properties. They have attracted the interests of many researchers and are becoming promising resources in the marine natural product research field. A bioassay-guided isolation from the crude extract of Verrucosispora sp. strain MS100047, isolated from sediments collected from the South China Sea, has led to the identification of a new salicylic derivative, glycerol 1-hydroxy-2,5-dimethyl benzoate (1), along with three known compounds, brevianamide F (2), abyssomicin B (3), and proximicin B (4). Compound 1 showed selective activity against methicillin-resistant Staphylococcus aureus (MRSA) with a minimum inhibitory concentration (MIC) value of 12.5 mu g/mL. Brevianamide F (2), which was isolated from actinomycete for the first time, showed a good anti-BCG activity with a MIC value of 12.5 mu g/mL that has not been reported previously in literatures. Proximicin B (4) showed significant anti-MRSA (MIC = 3.125 mu g/mL), anti-BCG (MIC = 6.25 mu g/mL), and anti-tuberculosis (TB) (MIC = 25 mu g/mL) activities. This is the first report on the anti-tubercular activities of proximicins. In addition, Verrucosispora sp. strain MS100047 was found to harbor 18 putative secondary metabolite gene clusters based on genomic sequence analysis. These include the biosynthetic loci encoding polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) consistent with abyssomicins and proximicins, respectively. The biosynthetic pathways of these isolated compounds have been proposed. These results indicate that MS100047 possesses a great potential as a source of active secondary metabolites.