韩静导师简介

 韩静  博士  项目研究员

 研究方向
   

极端微生物高值产物的合成及应用

 研究内容

解析嗜盐微生物独特的生理代谢途径，特别是生物可降解塑料聚羟基脂肪酸酯（PHA）的合成及调控机制，利用合成生物学策略改造嗜盐微生物底盘细胞，实现新型PHA的高效合成，并探索PHA在生物医学领域的高附加值应用。

 主持项目 

      主持在研国家自然科学基金面上项目2项，国家重点研发计划合成生物学专项项目课题和子课题各1项。已主持完成国家自然科学基金青年项目1项，国家自然科学基金面上项目1项。

  导师简介

 主要学习及工作经历 

工作经历  

2018.10-至今， 中国科学院微生物研究所，项目研究员

2011.7-2018.10， 中国科学院微生物研究所，副研究员

2008.7-2011.6， 中国科学院微生物研究所，助理研究员

2016.11-2017.10，哥伦比亚大学，访问学者

2013.10-2017.11，弗莱堡大学，访问学者

  教育经历 

2005.9-2008.7，中国科学院微生物研究所，博士

2002.9-2005.7，山东师范大学，硕士

1998.9-2002.7，山东师范大学，学士

 获奖情况

    2015年入选 “中国科学院青年创新促进会”会员

 近年代表性研究论文(*通讯作者) 

1. Qiong Xue^#, Xiao-Bin Liu^#, Yeh-Hsing Lao, Lin-Ping Wu, Dong Wang, Zhen-Qiang Zuo, Jun-Yu Chen, Jing Hou, Yuan-Yuan Bei, Xue-Fei Wu, Kam W. Leong, Hua Xiang,Jing Han*. Anti-infective biomaterials with surface-decorated tachyplesin I. Biomaterials, 2018,178:351-362. 

2.Han J*, Wu L, Liu X, Hou J, Zhao L, Chen J, Zhao D, Xiang H*. Biodegradation and biocompatibility of haloarchaea-produced poly (3-hydroxybutyrate-co-3-hydroxyvalerate) copolymers. Biomaterials,2017,139:172-186. 

3. Lin Lin, Junyu Chen, Ruchira Mitra, Quanxiu Gao, Feiyue Cheng, Tong Xu, Zhenqiang Zuo, Hua Xiang*,Jing Han*. Optimising PHBV biopolymer production in haloarchaeaviaCRISPRi-mediated redirection of carbon flux.Communications Biology,2021,4:1007. 

4.Han J#^,*, Wu LP#, Hou J, Zhao D, Xiang H*. Biosynthesis, characterization, and hemostasis potential of tailor-made poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced byHaloferax mediterranei. Biomacromolecules, 2015,16:578-588. 

5. Kim HS, Chen, JY, Wu LP, Wu JH, Xiang Hua*, Leong Kam,Jing Han*. Prevention of excessive scar formation using nanofibrous meshes made of biodegradable elastomer poly(3-hydroxybutyrate-co-3-hydroxyvalerate). Journal of Tissue Engineering, 2020,11:2041731420949332. 

6. Ruchi Mitra, Tong Xu, Guo-Qiang Chen, Hua Xiang*,Jing Han*. An updated overview on the regulatory circuits of polyhydroxyalkanoates synthesis. Microbial Biotechnology, 2021, doi:10.1111/1751-7915.13915. 

7. Ruchira Mitra, Tong Xu, Hua Xiang* andJing Han*. Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory. Microbial Cell Factories, 2020,19:86. 

8. Zuo Z, Xue Q, Zhou J, Zhao D,Han J* and Xiang H*. Engineering Haloferax mediterranei as an efficient platform for high level production of lycopene. Frontiers in Microbiology, 2018,9:2893. 

9. Junyu Chen, Ruchira Mitra, Shengjie Zhang，Zhenqiang Zuo, Lin Lin, Dahe Zhao, Hua Xiang*, andJing Han*. Unusual phosphoenolpyruvate (PEP) synthetase-like protein crucial to enhancement of polyhydroxyalkanoate accumulation in Haloferax mediterranei revealed by dissection of PEP-pyruvate interconversion mechanism. Applied and Environmental Microbiology, 2019,85:e00984-19. 

10. Hou J, Xiang H*,Han J*. Propionyl-CoA Carboxylase in Haloferax mediterranei: indispensability for propionyl-CoA assimilation and impacts on global metabolism. Applied and Environmental Microbiology, 2015,81:794-804. 

11. Hou J, Feng B,Han J*, Liu H, Zhao D, Zhou J, Xiang H*. Haloarchaeal type β-ketothiolases involved in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis inHaloferax mediterranei. Applied and Environmental Microbiology, 2013,79:5104-5111. 

12.Han J#, Hou J#, Zhang F, Ai G, Li M, Cai S, Liu H, Wang L, Wang Z, Zhang S, Cai L, Zhao D, Zhou J, Xiang H*. Multiple propionyl-CoA supplying pathways for production of bioplastic poly(3-hydroxybutyrate-co-3-hydroxyvalerate) inHaloferax mediterranei. Applied and Environmental Microbiology, 2013, 79: 2922-2931. 

13. Junyu Chen, Ruchira Mitra, Hua Xiang*, andJing Han*. Deletion of thepps-likegene activates the crypticphaCgenes inHaloferax mediterranei. Applied Microbiology and Biotechnology, 2020,104:9759-9771. 

14. Liu Xiao-Bin, Wu Lin-Ping, Hou Jing, Chen Jun-Yu,Han Jing*, and Xiang Hua*. Environmental biodegradation of haloarchaea-produced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in activated sludge. Applied Microbiology and Biotechnology, 2016,100: 6893-6902. 

15. Hou J,Han J*, Cai L, Zhou J, Lü Y, Jin C, Liu J, Xiang H*. 2013. Characterization of genes for chitin catabolism inHaloferax mediterranei. Applied Microbiology and Biotechnology, 2014,98:1185-1194. 

16.Han J#, Zhang F#, Hou J, Liu X, Li M, Liu H, Cai L, Zhang B, Chen Y, Zhou J, Hu S*, Xiang H*. Complete genome sequence of the metabolically versatile halophilic archaeonHaloferax mediterranei, a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) producer. Journal of Bacteriology, 2012,194:4463–4464. 

17.Han J, Hou J, Liu H, Cai S, Feng B, Zhou J, Xiang H*. Wide distribution among halophilic archaea of a novel polyhydroxyalkanoate synthase subtype with homology to bacterial type III synthases.Applied and Environmental Microbiology,2010, 76: 7811-7819. 

18.Han J, Li M, Hou J, Wu L, Zhou J, Xiang H*. Comparison of fourphaCgenes fromHaloferax mediterraneiand their function in different PHBV copolymer biosyntheses inHaloarcula hispanica.Saline Systems,2010, 6:9. 

19.Han J, Lu Q, Zhou L, Liu H, and Xiang H*. Identification of the polyhydroxyalkanoate (PHA)-Specific acetoacetyl-CoA reductase from multiple FabG paralogs inHaloarcula hispanicaand reconstruction of PHA biosynthetic pathway inHaloferax volcanii.Applied and Environmental Microbiology,2009, 75: 6168-6175. 

20. Lu Q^#,Han J^#, Zhou L, Zhou JandXiang H*.Genetic and biochemical characterization of the poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthase inHaloferax mediterranei.Journal of Bacteriology,2008, 190: 4173-4180. 

21.Han J^#, Lu Q^#, Zhou L, Zhou J and Xiang H*. Molecular characterization of thephaEC_Hmgenes, required for biosynthesis of poly(3-hydroxybutyrate) in the extremely halophilic  archaeonHaloarcula marismortui.Applied and Environmental Microbiology,2007,73:6058-6065. 

22.Han J,Qiu YZ, Liu DC*, Chen GQ*. EngineeredAeromonas hydrophilafor enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) withalterable monomers composition.FEMS Microbiology Letters,2004,239: 195-201.