Detection of glycan-binding proteins using glycan-functionalized quantum dots and gold nanoparticles----中国科学院微生物研究所

科研成果

论文题目:	Detection of glycan-binding proteins using glycan-functionalized quantum dots and gold nanoparticles
作者:	Zhang Zhenxing , Qi Xiaoxiao , Chai Jinfeng , Wu Peixing , Lv Xun, Cheng Shuihong, and Li Xuebing*
联系作者:
刊物名称:	Journal of Carbohydrate Chemistry
期:	37
卷:	4
页:	199-209
年份:	2018
影响因子:	0.983
论文下载:
摘要:	Many pathogens can use their glycan-binding proteins (GBPs) to interact with glycan receptors on host cell surface. Here, a novel strategy is presented for the detection of GBPs based on fluorescence resonance energy transfer (FRET). Using mannosefunctionalized quantum dots and mannose-functionalized gold nanoparticles, a FRET assay system was developed and a mannose-specific GBP concanavalin A could be detected in 5 min with a detection limit of 3 nM. This result indicated the potential for rapid and sensitive biosensing of various kinds of GBPs and pathogens by changing the glycan structure on the surface of QDs and AuNPs. [GRAPHICS] .