Direct and tunable modulation of protein levels in rice and wheat with a synthetic small molecule----中国科学院微生物研究所

科研成果

论文题目:	Direct and tunable modulation of protein levels in rice and wheat with a synthetic small molecule
作者:	Zhang Jingbo, Yin Kangquan, Sun Juan, Gao Jinlan, Du Qiuli, Li Huali, and Qiu Jin-Long*.
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刊物名称:	Plant Biotechnol J
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年份:	2017
影响因子:	6.657
论文下载:	http://onlinelibrary.wiley.com/doi/10.1111/pbi.12787/abstract;jsessionid=231CF50B71E1A0A8AF18E8678F0AF1CA.f04t03
摘要:	Direct control of protein level enables rapid and efficient analyses of gene functions in crops. Previously, we developed the RDDK-Shield1 (Shld1) system in the model plant Arabidopsis thaliana for direct modulation of protein stabilization using a synthetic small molecule. However, it was unclear whether this system is applicable to economically important crops. In this study, we show that the RDDK-Shld1 system enables rapid and tunable control of protein levels in rice and wheat. Accumulation of RDDK fusion proteins can be reversibly and spatio-temporally controlled by the synthetic small-molecule Shld1. Moreover, RDDK-Bar and RDDK-Pid3 fusions confer herbicide and rice blast resistance, respectively, in a Shld1-dependent manner. Therefore, the RDDK-Shld1 system provides a reversible and tunable technique for controlling protein functions and conditional expression of transgenes in crops.