| 论文题目: | Whole-cell biocatalysts by design |
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| 作者: | Lin Baixue* , and Tao Yong*. |
| 联系作者: | |
| 刊物名称: | Microbial Cell Factories |
| 期: | 16 |
| 卷: | |
| 页: | |
| 年份: | 2017 |
| 影响因子: | 4.185 |
| 论文下载: | https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0724-7 |
| 摘要: | Whole-cell biocatalysts provide unique advantages and have been widely used for the efficient biosynthesis of value-added fine and bulk chemicals, as well as pharmaceutically active ingredients. What is more, advances in synthetic biology and metabolic engineering, together with the rapid development of molecular genetic tools, have brought about a renaissance of whole-cell biocatalysis. These rapid advancements mean that whole-cell biocatalysts can increasingly be rationally designed. Genes of heterologous enzymes or synthetic pathways are increasingly being introduced into microbial hosts, and depending on the complexity of the synthetic pathway or the target products, they can enable the production of value-added chemicals from cheap feedstock. Metabolic engineering and synthetic biology efforts aimed at optimizing the existing microbial cell factories concentrate on improving heterologous pathway flux, precursor supply, and cofactor balance, as well as other aspects of cellular metabolism, to enhance the efficiency of biocatalysts. In the present review, we take a critical look at recent developments in whole-cell biocatalysis, with an emphasis on strategies applied to designing and optimizing the organisms that are increasingly modified for efficient production of chemicals. |
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