| 论文题目: | A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays |
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| 作者: | Li Shanshan , Zhou Li , Yao Yongpeng , Fan Keqiang , Li Zilong , Zhang Lixin , Wang Weishan , and Yang Keqian*. |
| 联系作者: | |
| 刊物名称: | Chemical Communications |
| 期: | |
| 卷: | 53 |
| 页: | 99-102 |
| 年份: | 2017 |
| 影响因子: | 6.628 |
| 论文下载: | http://pubs.rsc.org/en/Content/ArticleLanding/2017/CC/C6CC07244E#!divAbstract |
| 摘要: | A wide range of chemicals can be sensed by allosteric transcription factors (aTFs) in bacteria. Herein, we report a biosensing platform by using isolated aTFs as recognition elements in vitro. Moreover, a general strategy to increase the sensitivity of the aTF-based biosensors is provided. As a proof-of-concept, we obtained by far the most sensitive uric acid and oxytetracycline biosensors by using aTF HucR and OtrR as recognition elements, respectively. As a large number of aTFs are present in bacteria, our work opens a novel route to develop sensitive aTF-based biosensors. |
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