| 论文题目: | Kocuria oceani sp nov., isolated from a deep- sea hydrothermal plume |
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| 作者: | Zhang Limin , Xi Lijun , Ruan Jisheng , and Huang Ying*. |
| 联系作者: | |
| 刊物名称: | International Journal of Systematic and Evolutionary Microbiology |
| 期: | |
| 卷: | 67 |
| 页: | 164-169 |
| 年份: | 2017 |
| 影响因子: | 2.782 |
| 论文下载: | http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001599 |
| 摘要: | Three strains, FXJ8.095(T), FXJ8.057 and H201, were isolated from deep-sea hydrothermal plume water collected at the Southwest Indian Ridge at a depth of 2800 m. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates formed a closely related subcluster within the genus Kocuria. The 16S rRNA gene sequence of strain FXJ8.095(T) shared 99.90 and 99.60% similarity with those of strains FXJ8.057 and H201, respectively, and 98.81, 98.75, 98.68 and 98.10% with those of `Kocuria sediminis' JCM 17929, Kocuria flava HO-9041(T), Kocuria turfanensis HO-9042(T) and Kocuria rosea JCM 11614(T), respectively. DNA-DNA hybridization values among the three new isolates were higher than 70 %, while the values between each of the isolates and the closely related type strains were well below 70 %. Random amplified polymorphic DNA fingerprint patterns and a combination of physiological and biochemical properties also distinguished the isolates from the related species. The major cellular fatty acids of the isolates were anteiso-C-15:0 and iso-C-15:0, the predominant menaquinones were MK-7(H-2) and MK-8(H-2), and the main polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C contents of strains FXJ8.095(T), FXJ8.057 and H201 were 75.6, 72.8 and 70.4 mol%, respectively. Based on the phylogenetic, phenotypic and chemotaxonomic data, we propose to classify the three strains in a novel species named Kocuria oceani sp. nov., with FXJ8.095(T) (= CGMCC 4.6946(T) = DSM 24949(T)) as the type strain. |
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