The Regulatory Subunit of Protein Kinase A (Bcyl) in Candida albicans Plays Critical Roles in Filamentation and White-Opaque Switching but Is Not Essential for Cell Growth----中国科学院微生物研究所

科研成果

论文题目:	The Regulatory Subunit of Protein Kinase A (Bcyl) in Candida albicans Plays Critical Roles in Filamentation and White-Opaque Switching but Is Not Essential for Cell Growth
作者:	Ding Xuefen , Cao Chengjun , Zheng Qiushi , and Huang Guanghua*.
联系作者:
刊物名称:	Frontiers in Microbiology
期:	7
卷:
页:
年份:	2017
影响因子:	4.36
论文下载:	http://journal.frontiersin.org/article/10.3389/fmicb.2016.02127/full
摘要:	The conserved cAMP-dependent protein kinase (PKA) is composed of the regulatory and catalytic subunits and acts as the central component of the cAMP signaling pathway. In the human fungal pathogen Candida albicans, the PKA regulatory subunit Bcyl plays a critical role in the regulation of cell differentiation and death. It has long been considered that Bcyl is essential for cell viability in C. albicans. In the current study, surprisingly, we found that Bcyl is not required for cell growth, and we successfully generated a bcyl/bcyl null mutant in C. albicans. Deletion of BCY1 leads to multiple cellular morphologies and promotes the development of filaments. Filamentous and smooth colonies are two typical morphological types of the bcyl/bcyl mutant, which can undergo spontaneous switching between the two types. Cells of filamentous colonies grow better on a number of different culture media and have a higher survival rate than cells of smooth colonies. In addition, deletion of BCY1 significantly increased the frequency of white-to-opaque switching on N-acetylglucosamine (GIcNAc)-containing medium. The bcyl/bcyl null mutant generated herein provides the field a new resource to study the biological functions of the cAMP signaling pathway in C. albicans.