| 论文题目: | Molecular determinants of human neutralizing antibodies isolated from a patient infected with Zika virus |
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| 作者: | Wang Qihui, Yang Huabing, Liu Xiaoqing, Dai Lianpan, Ma Tong, Qi Jianxun, Wong Gary, Peng Ruchao, Liu Sheng, Li Junfu, Li Shihua, Song Jian, Liu Jianying, He Jianhua, Yuan Hui, Xiong Ying, Liao Yong, Li Jianhua, Yang Jianping, Tong Zhou, Griffin Bryan D, Bi Yuhai, Liang Mifang, Xu Xiaoning, Qin Chuan, Cheng Gong, Zhang Xinzheng, Wang Peiyi, Qiu Xiangguo, Kobinger Gary, Shi Yi, Yan Jinghua*, and Gao George F*. |
| 联系作者: | |
| 刊物名称: | Sci Transl Med |
| 期: | 8 |
| 卷: | |
| 页: | 369ra179 |
| 年份: | 2016 |
| 影响因子: | 15.051 |
| 论文下载: | |
| 摘要: | The 2015-2016 outbreak of Zika virus (ZIKV) disease has affected many countries and is a major public health concern. ZIKV is associated with fetal microcephaly and neurological complications, and countermeasures are needed to treat and prevent ZIKV infection. We report the isolation of 13 specific human monoclonal antibodies from a single patient infected with ZIKV. Two of the isolated antibodies (Z23 and Z3L1) demonstrated potent ZIKV-specific neutralization in vitro without binding or neutralizing activity against strains 1 to 4 of dengue virus, the closest relative to ZIKV. These two antibodies provided postexposure protection to mice in vivo. Structural studies revealed that Z23 and Z3L1 bound to tertiary epitopes in envelope protein domain I, II, or III, indicating potential targets for ZIKV-specific therapy. Our results suggest the potential of antibody-based therapeutics and provide a structure-based rationale for the design of future ZIKV-specific vaccines. |
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