Engineering deacetoxycephalosporin C synthase as a catalyst for the bioconversion of penicillins----中国科学院微生物研究所

科研成果

论文题目:	Engineering deacetoxycephalosporin C synthase as a catalyst for the bioconversion of penicillins
作者:	Fan, Keqiang Lin, Baixue Tao, Yong Yang, Keqian
联系作者:	Yang, Keqian*
刊物名称:	J Ind Microbiol Biotechnol
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卷:
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年份:	2016
影响因子:	2.732
论文下载:	http://link.springer.com/article/10.1007%2Fs10295-016-1857-0
摘要:	7-aminodeacetoxycephalosporanic acid (7-ADCA) is a key intermediate of many clinically useful semisynthetic cephalosporins that were traditionally prepared by processes involving chemical ring expansion of penicillin G. Bioconversion of penicillins to cephalosporins using deacetoxycephalosporin C synthase (DAOCS) is an alternative and environmentally friendly process for 7-ADCA production. Arnold Demain and co-workers pioneered such a process. Later, protein engineering efforts to improve the substrate specificity and catalytic efficiency of DAOCS for penicillins have been made by many groups, and a whole cell process using Escherichia coli for bioconversion of penicillins has been developed.