Heterologous expression of oxytetracycline biosynthetic gene cluster in Streptomyces venezuelae WVR2006 to improve production level and to alter fermentation process----中国科学院微生物研究所

科研成果

论文题目:	Heterologous expression of oxytetracycline biosynthetic gene cluster in Streptomyces venezuelae WVR2006 to improve production level and to alter fermentation process
作者:	Yin, Shouliang Li, Zilong Wang, Xuefeng Wang, Huizhuan Jia, Xiaole Ai, Guomin Bai, Zishang Shi, Mingxin Yuan, Fang Liu, Tiejun Wang, Weishan Yang, Keqian
联系作者:
刊物名称:	Appl Microbiol Biotechnol
期:
卷:
页:
年份:	2016
影响因子:	3.882
论文下载:	http://link.springer.com/article/10.1007%2Fs00253-016-7873-1
摘要:	Heterologous expression is an important strategy to activate biosynthetic gene clusters of secondary metabolites. Here, it is employed to activate and manipulate the oxytetracycline (OTC) gene cluster and to alter OTC fermentation process. To achieve these goals, a fast-growing heterologous host Streptomyces venezuelae WVR2006 was rationally selected among several potential hosts. It shows rapid and dispersed growth and intrinsic high resistance to OTC. By manipulating the expression of two cluster-situated regulators (CSR) OtcR and OtrR and precursor supply, the OTC production level was significantly increased in this heterologous host from 75 to 431 mg/l only in 48 h, a level comparable to the native producer Streptomyces rimosus M4018 in 8 days. This work shows that S. venezuelae WVR2006 is a promising chassis for the production of secondary metabolites, and the engineered heterologous OTC producer has the potential to completely alter the fermentation process of OTC production.