| 论文题目: | In vitro assembly of Ebola virus nucleocapsid-like complex expressed in E. coli |
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| 作者: | Peng, Ruchao Zhu, Tengfei Oladejo, Babayemi Olawale Musyoki, Abednego Moki Cui, Yingzi Shi, Yi Wang, Peiyi Gao, George Fu |
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| 刊物名称: | Protein Cell |
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| 年份: | 2016 |
| 影响因子: | 3.043 |
| 论文下载: | http://link.springer.com/article/10.1007%2Fs13238-016-0314-1 |
| 摘要: | Ebola virus (EBOV) harbors an RNA genome encapsidated by nucleoprotein (NP) along with other viral proteins to form a nucleocapsid complex. Previous Cryo-eletron tomography and biochemical studies have shown the helical structure of EBOV nucleocapsid at nanometer resolution and the first 450 amino-acid of NP (NPDelta451-739) alone is capable of forming a helical nucleocapsid-like complex (NLC). However, the structural basis for NP-NP interaction and the dynamic procedure of the nucleocapsid assembly is yet poorly understood. In this work, we, by using an E. coli expression system, captured a series of images of NPDelta451-739 conformers at different stages of NLC assembly by negative-stain electron microscopy, which allowed us to picture the dynamic procedure of EBOV nucleocapsid assembly. Along with further biochemical studies, we showed the assembly of NLC is salt-sensitive, and also established an indispensible role of RNA in this process. We propose the diverse modes of NLC elongation might be the key determinants shaping the plasticity of EBOV virions. Our findings provide a new model for characterizing the self-oligomerization of viral nucleoproteins and studying the dynamic assembly process of viral nucleocapsid in vitro. |
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