Hyphopodium-Specific VdNoxB/VdPls1-Dependent ROS-Ca2+Signaling Is Required for Plant Infection byVerticillium dahliae----中国科学院微生物研究所

科研成果

论文题目:	Hyphopodium-Specific VdNoxB/VdPls1-Dependent ROS-Ca2+Signaling Is Required for Plant Infection byVerticillium dahliae
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刊物名称:	Plos Pathogens
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年份:	2016
影响因子:	7.758
论文下载:	http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1005793
摘要:	Author Summary Beyond the harmful roles in pathological processes and aging, growing evidence indicates that reactive oxygen species (ROS) play a pivotal role in physiological processes, including cell differentiation and development in fungi. Verticillium dahliae Kleb., a soil-born phytopathogenic fungus, germinates larger numbers of hyphae on root surface, a few of which differentiate into hyphopodia during initial colonization. In this study, we verified that a specific membrane-bound NADPH oxidase complex (Nox), composed of the catalytic subunit VdNoxB and the tetraspaninVdPls1, is responsible for the ROS burst and penetration peg differentiation within the hyphopodium, which is essential for redirecting fungal growth toward host cells to penetrate cotton roots and to colonize the host vascular system. Moreover, we found that VdPls1/VdNoxB-mediated ROS production elevates tip-high cytoplasmic free calcium ions (Ca 2+ ) in hyphopodia to activate conservation calcineurin-Crz1 signaling for the establishment polarized penetration peg formation. In plant root-hair cells, endogenous ROS elevation activates tip-high Ca 2+ for root-hair cell polarity and elongation. Taken together, we propose that Ca 2+ , as a second messenger for transduction of the apical ROS signal, is a hallmark and common regulatory mechanism for apical growth in eukaryotes.