Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling----中国科学院微生物研究所

科研成果

论文题目:	Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
作者:	Jiang, Jing Chen, Xinchun An, Hongjuan Yang, Bingfen Zhang, Fuping Cheng, Xiaoxing
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刊物名称:	Sci Rep
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年份:	2016
影响因子:	5.525
论文下载:	http://www.nature.com/articles/srep32320#author-information
摘要:	The functions of MAIT cells at the site of Mycobacterium tuberculosis infection in humans are still largely unknown. In this study, the phenotypes and immune response of MAIT cells from tuberculous pleural effusions and peripheral blood were investigated. MAIT cells in tuberculous pleural effusions had greatly enhanced IFN-gamma, IL-17F and granzyme B response compared with those in peripheral blood. The level of IFN-gamma response in MAIT cells from tuberculous pleural effusions was inversely correlated with the extent of tuberculosis infection (p = 0.0006). To determine whether cytokines drive the immune responses of MAIT cells at the site of tuberculosis infection, the role of IL-1beta, IL-2, IL-7, IL-12, IL-15 and IL-18 was investigated. Blockade of IL-2, IL-12 or IL-18 led to significantly reduced production of IFN-gamma and/or granzyme B in MAIT cells from tuberculous pleural effusions. Majority of IL-2-producing cells (94.50%) in tuberculous pleural effusions had phenotype of CD3(+)CD4(+), and most IL-12p40-producing cells (91.39%) were CD14(+) cells. MAIT cells had significantly elevated expression of gammac receptor which correlated with enhanced immune responses of MAIT cells. It is concluded that MAIT cells from tuberculous pleural effusions exhibited highly elevated immune response to Mtb antigens, which are controlled by cytokines produced by innate/adaptive immune cells.