| 论文题目: | Involvement of an octose ketoreductase and two acyltransferases in the biosynthesis of paulomycins |
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| 作者: | Li Jine, Wang Min, Ding Yong, Tang Yue, Zhang Zhiguo*, and Chen Yihua*. |
| 联系作者: | |
| 刊物名称: | Sci Rep |
| 期: | |
| 卷: | 6 |
| 页: | 21180 |
| 年份: | 2016 |
| 影响因子: | 5.597 |
| 论文下载: | http://www.nature.com/articles/srep21180 |
| 摘要: | C-4 hydroxyethyl branched octoses have been observed in polysaccharides of several genera of gram negative bacteria and in various antibiotics produced by gram positive bacteria. The C-4 hydroxyethyl branch was proposed to be converted from C-4 acetyl branch by an uncharacterized ketoreduction step. Paulomycins (PAUs) are glycosylated antibiotics with potent inhibitory activity against gram positive bacteria and are structurally defined by its unique C-4' hydroxyethyl branched paulomycose moiety. A novel aldo-keto-reductase, Pau7 was characterized as the enzyme catalyzing the stereospecific ketoreduction of 7'-keto of PAU E (1) to give the C-4' hydroxyethyl branched paulomycose moiety of PAU F (2). An acyltransferase Pau6 further decorates the C-4' hydroxyethyl branch of paulomycose moiety of 2 by attaching various fatty acyl chains to 7'-OH to generate diverse PAUs. In addition, another acyltransferase Pau24 was proposed to be responsible for the 13-O-acetylation of PAUs. |
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