| 论文题目: | Functional Characterization of a Dihydroflavanol 4-Reductase from the Fiber of Upland Cotton (Gossypium hirsutum) |
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| 作者: | Wang Le#, Zhu Yue#, Wang Peng, Fan Qiang, Wu Yao, Peng Qing-Zhong, Xia Gui-Xian*, and Wu Jia-He*. |
| 联系作者: | |
| 刊物名称: | Molecules |
| 期: | 2 |
| 卷: | 21 |
| 页: | |
| 年份: | 2016 |
| 影响因子: | 2.791 |
| 论文下载: | http://www.mdpi.com/1420-3049/21/2/32 |
| 摘要: | Dihydroflavanol 4-reductase (DFR) is a key later enzyme involved in two polyphenols' (anthocyanins and proanthocyanidins (PAs)) biosynthesis, however it is not characterized in cotton yet. In present reports, a DFR cDNA homolog (designated as GhDFR1) was cloned from developing fibers of upland cotton. Silencing GhDFR1 in cotton by virus-induced gene silencing led to significant decrease in accumulation of anthocyanins and PAs. More interestingly, based on LC-MS analysis, two PA monomers, (-)-epicatachin and (-)-epigallocatachin, remarkably decreased in content in fibers of GhDFR1-silenced plants, but two new monomers, (-)-catachin and (-)-gallocatachin were present compared to the control plants infected with empty vector. The ectopic expression of GhDFR1 in an Arabidopsis TT3 mutant allowed for reconstruction of PAs biosynthesis pathway and led to accumulation of PAs in seed coat. Taken together, these data demonstrate that GhDFR1 contributes to the biosynthesis of anthocyanins and PAs in cotton. |
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