Vibrio salilacus sp nov., a new member of the Anguillarum clade with six alleles of the 16S rRNA gene from a saline lake----中国科学院微生物研究所

科研成果

论文题目:	Vibrio salilacus sp nov., a new member of the Anguillarum clade with six alleles of the 16S rRNA gene from a saline lake
作者:	Zhong Zhi-Ping , Liu Ying , Liu Hong-Can , Wang Fang , Zhou Yu-Guang , and Liu Zhi-Pei*.
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刊物名称:	International Journal of Systematic and Evolutionary Microbiology
期:
卷:	65
页:	2653-2660
年份:	2015
影响因子:	2.403
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摘要:	A Gram-stain-negative, catalase- and oxidase-positive, facultatively aerobic bacterium, strain DSG-S6(T), was isolated from Dasugan Lake (salinity 3.1 %, w/w), China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain DSG-S6(T) were non-sporeforming, slightly bent rods, and motile by means of a single polar flagellum. Growth occurred in the presence of 0-7.0 % (w/v) NaCl (optimum, 2.0 %), at 4-35 degrees C (optimum, 30 degrees C) and at pH 6.0-10.5 (optimum, pH 8.0-8.5). C-16:0, C-18 1 omega 7C and C-16 (:) (1) omega 7c and/or C-16 (:) (1) omega 6c were the major fatty acids. Six alleles of the 16S rRNA gene sharing 98.9-99.9 % similarity were detected in strain DSG-S6(T), which showed highest 16S rRNA gene sequence similarity to Vibrio aestuarianus ATCC 35048(T) (97.7 %), then to Vibrio pacinhl LMG 19999(T) (97.6 %) and Vibrio metschnikovii CIP 69.14(T) (96.8 %). Multilocus sequence analysis of four housekeeping genes and 16S rRNA genes clearly clustered it as a member of the Anguillarum clade. Mean DNA DNA relatedness between strain DSG-S6T and V. aestuarianus NBRC 15629(T), V. pacinii CGMCC 1.12557(T) and V. metschnikovii JCM 21189(T) was 20.6+2.3, 38.1+3.5 and 24.2+2.8 %, respectively. The DNA G -1-C content was 46.8 mol% (7-,n). Based on the data, it is concluded that strain DSG-S61 represents a novel species of the genus Vibrio, for which the name Vibrio salilacus sp. nov. is proposed. The type strain is DSG-S6T (=CGMCC 1.12427T=JCM 19265T).