| 论文题目: | Design and Application of a Novel High-throughput Screening Technique for 1-Deoxynojirimycin |
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| 作者: | Jiang Peixia Mu Shanshan, Li Heng, Li Youhai, Feng Congmin, Jin Jian-Ming*, Tang Shuang-Yan*. |
| 联系作者: | |
| 刊物名称: | Sci Rep |
| 期: | 5 |
| 卷: | |
| 页: | 8563 |
| 年份: | 2015 |
| 影响因子: | 5.597 |
| 论文下载: | http://www.nature.com/srep/2015/150224/srep08563/full/srep08563.html |
| 摘要: | High-throughput screening techniques for small molecules can find intensive applications in the studies of biosynthesis of these molecules. A sensitive, rapid and cost-effective technique that allows high-throughput screening of endogenous production of the natural iminosugar 1-deoxynojirimycin (1-DNJ), an alpha-glucosidase inhibitor relevant to the pharmaceutical industry, was developed in this study, based on the inhibitory effects of 1-DNJ on the activity of the beta-glycosidase LacS from Sulfolobus solfataricus. This technique has been demonstrated effective in engineering both the key enzyme and the expression levels of enzymes in the 1-DNJ biosynthetic pathway from Bacillus atrophaeus cloned in E. coli. Higher biosynthetic efficiency was achieved using directed evolution strategies. |
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