| 论文题目: | A novel chemoreceptor MCP2983 from Comamonas testosteroni specifically binds to cis-aconitate and triggers chemotaxis towards diverse organic compounds |
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| 作者: | Ni Bin Huang Zhou, Wu Yu-Fan, Fan Zheng, Jiang Cheng-Ying*, Liu Shuang-Jiang*. |
| 联系作者: | |
| 刊物名称: | Applied Microbiology and Biotechnology |
| 期: | 99 |
| 卷: | 6 |
| 页: | 2773-2781 |
| 年份: | 2015 |
| 影响因子: | 3.848 |
| 论文下载: | http://link.springer.com/article/10.1007%2Fs00253-014-6216-3 |
| 摘要: | Comamonas testosteroni CNB-1 behaves chemotactically toward a wide range of organic compounds, and 19 methyl-accepting chemotaxis proteins (MCPs) were annotated from the genome of strain CNB-2, a plasmid-curing derivative from strain CNB-1. The MCP-free mutant CNB-1 Delta 20 completely lost its chemotactic responses. In this study, we found that a chemoreceptor, namely MCP2983, restored chemotactic responses toward nine carboxylic acids and ten aromatic compounds to CNB-1 Delta 20. Isothermal titration calorimetry analysis indicated that the ligand-binding domain (LBD) of MCP2983 specifically binds to cis-aconitate but not other tested compounds. Deletion of the LBD of MCP2983 impaired chemotactic responses toward cis-aconitate as well as other tested compounds, indicating that the LBD of MCP2983 was essential for triggering chemotactic responses. Five amino acid residues (M-81, S-156, E-157, I-158, and L-159) that are located at a putative ligand-binding pocket were identified to be involved in binding to cis-aconitate. So far, the MCP2983 represents the sole biochemically identified chemoreceptor that specifically binds to cis-aconitate and is able to trigger chemotaxis towards diverse organic compounds. |
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