Pseudomonas salina sp. nov., isolated from a salt lake in Qaidam basin, China----中国科学院微生物研究所

科研成果

论文题目:	Pseudomonas salina sp. nov., isolated from a salt lake in Qaidam basin, China
作者:	Zhong Zhi-Ping, Liu Ying, Hou Ting-Ting, Liu Hong-Can, Zhou Yu-Guang, Wang Fang, and Liu Zhi-Pei*.
联系作者:
刊物名称:	Int J Syst Evol Microbiol
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年份:	2015
影响因子:	2.403
论文下载:	http://ijs.sgmjournals.org/content/early/2015/05/18/ijs.0.000341
摘要:	A Gram-staining-negative, facultative aerobic bacterium, strain XCD-X85T, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9 %, w/w) in Qaidam basin, Qinghai province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X85T were non-endospore-forming rods, 0.4-0.6 microm wide and 1.0-1.6 microm long, and motile by means of a single polar flagellum. Strain XCD-X85T was catalase- and oxidase-positive. Growth was observed in the presence of 0-12.0 % (w/v) NaCl (optimum, 1.0-2.0 %) and at 4-35 degrees C (optimum, 25-30 degrees C) and pH 6.5-10.5 (optimum, pH 8.0-8.5). Strain XCD-X85T contained (>10 %) summed feature 8 (C18:1omega7c and/or C18:1omega6c), C12:0, C16:0 and summed feature 3 (C16:1omega7c and/or C16:1omega6c) as the predominant fatty acids. The major respiratory quinone was ubiquinone 9 (Q-9). The major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and diphosphatidglycerol (DPG). The DNA G+C content was 57.4 mol%. Phylogenetic trees based on 16S rRNA gene sequences comparison showed that strain XCD-X85T was associated with the genus Pseudomonas, and showed highest 16S rRNA gene sequence similarities to P. pelagia CL-AP6T (99.0 %) and P. bauzanensis BZ93T (96.8 %). DNA-DNA relatedness of strain XCD-X85T to P. pelagia JCM 15562T was 19 +/- 1 %. Based on the data presented above, it is concluded that strain XCD-X85T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas salina sp. nov. is proposed. The type strain is XCD-X85T (= CGMCC 1.12482T = JCM 19469T).