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论文题目: Marivita lacus sp. nov., isolated from a saline lake
作者: Zhi-Ping Zhong, Ying Liu, Ting-Ting Hou, Hong-Can Liu, Yu-Guang Zhou, Fang Wang, and Zhi-Pei* Liu.
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刊物名称: Int J Syst Evol Microbiol
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年份: 2015
影响因子: 2.403
论文下载: http://ijs.sgmjournals.org/content/early/2015/03/19/ijs.0.000195
摘要: A Gram-staining-negative, strictly heterotrophic and aerobic bacterium, strain TS-T44T, was isolated from a saline lake, Tuosu Lake in Qaidam basin, Qinghai province, China. Its taxonomic position was investigated using a polyphasic approach. Cells of strain TS-T44T were non-endospore-forming, non-motile rods, 0.8-1.2 microm wide and 1.2-3.0 microm long. Catalase- and oxidase-positive. Growth occurred in the presence of NaCl up to 8 % (w/v, optimum, 3.0 %), and at 15-35 degrees C (optimum, 25 degrees C) and pH 7.0-10.0 (optimum, pH 7.5-8.5). C18:1omega7c was the only predominant fatty acid. The major respiratory quinone was Q-10. The major polar lipids were phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), an unidentified aminolipid and an unknown lipid. The DNA G+C content was 65.5 mol % (Tm). Phylogenetic trees based on 16S rRNA gene sequences showed that strain TS-T44T was associated with the genus Marivita, and showed highest sequence similarity to M. cryptomonadis CL-SK44T (97.7 %), M. litorea CL-JM1T (97.5 %), M. geojedonensis DPG-138T (97.3 %), and <70 % to others. DNA-DNA relatedness of strain TS-T44T to M. cryptomonadis JCM 15447T, M. litorea JCM 15446T and M. geojedonensis KCTC 23882T was 23 +/- 3 %, 33 +/- 4 % and 35 +/- 2 %, respectively. Based on the data presented above, it is concluded that strain TS-T44T represents a novel species of the genus Marivita, for which the name Marivita lacus sp. nov. is proposed. The type strain is TS-T44T (= CGMCC 1.12478T= JCM 19516T).