| 论文题目: | Metallosphaera tengchongensis sp. nov., an acidothermophilic archaeon isolated from a hot spring |
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| 作者: | Peng Tang-Jian Liu Li-Jun, Liu Chang, Yang Zhi-Fang, Liu Shuang-Jiang, Jiang Cheng-Ying*. |
| 联系作者: | |
| 刊物名称: | Int J Syst Evol Microbiol |
| 期: | 65 |
| 卷: | Pt 2 |
| 页: | 537-42 |
| 年份: | 2015 |
| 影响因子: | 2.403 |
| 论文下载: | http://ijs.sgmjournals.org/content/65/Pt_2/537.long |
| 摘要: | Two novel acidothermophilic archaea, strains Ric-A(T) and Ric-F, were isolated from muddy water samples of a sulfuric hot spring located in Tengchong County, Yunnan Province, PR China. The strains were aerobic and facultatively chemolithoautotrophic. Both strains could oxidize S(0) and K2S4O6 for autotrophic growth, and could use organic materials for heterotrophic growth. Growth was observed at 55-75 degrees C and pH 1.5-6.5. The strains could oxidize metal sulfide ores, showing their potential in bioleaching. The DNA G+C contents of strains Ric-A(T) and Ric-F were 41.8 and 41.6 mol%, respectively. Analysis of 16S rRNA gene sequences showed that the two strains shared 99.8 % sequence similarity to each other, but <97 % to other known species of the genus Metallosphaera. DNA-DNA hybridization indicated that the isolates were different strains of a novel species of the genus Metallosphaera. Strains Ric-A(T) and Ric-F also shared a number of physiological and biochemical characteristics that distinguished them from recognized species of the genus Metallosphaera. On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons with their closest relatives, it was concluded that strains Ric-A(T) and Ric-F represent a novel species of the genus Metallosphaera, for which the name Metallosphaera tengchongensis sp. nov. is proposed. The type strain is Ric-A(T) ( = NBRC 109472(T) = CGMCC 1.12287(T)). |
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