| 论文题目: | Characterization and homologous overexpression of an N-acetylglucosaminidase Nag1 from Trichoderma reesei |
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| 作者: | Chen Fei , Chen Xiu-Zhen , Qin Li-Na , Tao Yong , and Dong Zhi-Yang*. |
| 联系作者: | |
| 刊物名称: | Biochemical and Biophysical Research Communications |
| 期: | 459 |
| 卷: | 2 |
| 页: | 184-188 |
| 年份: | 2015 |
| 影响因子: | 2.382 |
| 论文下载: | http://www.sciencedirect.com/science/article/pii/S0006291X14022487 |
| 摘要: | Trichbderma reesei is thought to be a promising recombinant host for the production and secretion of complex proteins due to its ability to secrete large amounts of proteins. In this study we identified a functional N-acetyl-beta-glucosaminidase (NAGase) gene Nag1 in T. reesei. Nag1, a putative gene encoding a GH 20 family NAGase in T. reesei, was cloned and homologous overexpressed in the T. reesei RutC30 Delta U3 with a strong cellobiohydrolase1 gene (cbh1) promoter. Nag1 was secreted in its active form and the highest expression level was around 499.85 IU/ml. Nag1 has a molecular mass of 80 kDa. The optimum pH and temperature were 4.0 and 60 degrees C, respectively. (C) 2014 Elsevier Inc. All rights reserved. |
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