Methylopila henanense sp nov., a novel methylotrophic bacterium isolated from tribenuron methyl-contaminated wheat soil----中国科学院微生物研究所

科研成果

论文题目:	Methylopila henanense sp nov., a novel methylotrophic bacterium isolated from tribenuron methyl-contaminated wheat soil
作者:	Wang Ya-Nan* Tian Wen-Yu, He Wei-Hong, Chen Guo-Can, An Ming-Li, Jia Bin, Liu Li, Zhou Yang, Liu Shuang-Jiang.
联系作者:
刊物名称:	Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
期:	107
卷:	2
页:	329-336
年份:	2015
影响因子:	1.909
论文下载:	http://link.springer.com/article/10.1007%2Fs10482-014-0331-0
摘要:	A bacterial strain, designated LYBFD3-16A2(T), was isolated from tribenuron methyl contaminated wheat soil. Cells were observed to be Gram-negative short rods with a single flagellum. The strain was found to utilize methanol, glucose, maltose and mannitol as carbon and energy sources, and utilized glutamate, leucine, phenylalanine as organic nitrogen sources. Strain LYBFD3-16A2(T) was found to be aerobic, to form urease, produce hydrogen sulfide and reduce nitrate to nitrite. The indole test in tryptone broth was observed to be positive. The major cellular fatty acids were identified as C-18:1 omega 7c (81.3 %), 11-methylC(18:1 omega 7c) (7.9 %), C-18:0 (3.0 %) and C-16:0 (3.0 %). The major phospholipids were identified as phosphatidylcholine, phosphatidylethanolamine, phosphatidyglycerol and diphosphatidylglycerol. The main ubiquinone was identified as Q-10. The DNA G+C content was determined to be between 70.2 and 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated the affiliation of strain LYBFD3-16A2 to members of the genus Methylopila. The DNA-DNA hybridization values of the novel strain with the type strains of the most closely related species Methylopila musalis MUSA(T) and Methylopila jiangsuensis JZL-4(T) were 35.4 % and 31.4 % respectively. The genotypic and phenotypic characterization, along with chemotaxonomic properties of strain LYBFD3-16A2(T), showed that the strain represents a novel species of the genus Methylopila for which the name Methylopila henanense sp. nov. is proposed. The type strain is LYBFD3-16A2(T) (=CGMCC1.10703(T) = LMG 25959(T)).