Engineering of a hybrid route to enhance shikimic acid production in Corynebacterium glutamicum----中国科学院微生物研究所

科研成果

论文题目:	Engineering of a hybrid route to enhance shikimic acid production in Corynebacterium glutamicum
作者:	Zhang Bo, Jiang Cheng-Ying, Liu Yi-Ming, Liu Chang, and Liu Shuang-Jiang*.
联系作者:
刊物名称:	Biotechnol Lett
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年份:	2015
影响因子:	1.838
论文下载:	http://link.springer.com/article/10.1007%2Fs10529-015-1852-y
摘要:	OBJECTIVES: To exploit the archaeal shikimic acid (SA) synthesis pathway toenhance SA production in Corynebacterium glutamicum. RESULTS: Genetic cassettes were constructed that encoded a hybrid route for SA synthesis, which were composed of the archaeal 6-deoxy-5-ketofructose 1-phosphate pathway and the bacterial SA pathway. Corynebacterium glutamicum strains that expressed the hybrid route increased SA production by 57 %. A recombinant strain of C. glutamicum that simultaneously overexpressed the hybrid route and its native SA pathway produced 4.7 gSA/l, while C. glutamicum with only the native SA pathway produced 3.7 gSA/l. CONCLUSIONS: A new hybrid route for SA production was successfully constructed, that effectively increased SA production in C. glutamicum.