| 论文题目: | Engineering of Ralstonia eutropha for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose |
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| 作者: | Zhang Ying-Zi Liu Gui-Ming, Weng Wei-Qi, Ding Jiu-Yuan*, Liu Shuang-Jiang*. |
| 联系作者: | |
| 刊物名称: | Journal of Biotechnology |
| 期: | 195 |
| 卷: | |
| 页: | 82-88 |
| 年份: | 2015 |
| 影响因子: | 3.221 |
| 论文下载: | |
| 摘要: | Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with Ralstonia eutropha relies on the addition of propionate during fermentation, and propionate consumption is one of the major factors affecting the cost of PHBV production. In this study, 7 strains were obtained by genetic manipulating the methylcitric acid cycle and the methylmalonyl-CoA pathway in R. eutropha. Disruption of prpC1 and prpC2 genes did not affect cell growth and PHBV accumulation. All 7 strains were able to accumulation high amounts of PHBVs with 3HV fractions of 0.41-29.1 mol% during cultivation in flasks. Fermentation in 7.5-L fermenter showed that genetically engineered Rem-8 was able to yield biomass of 132.8 CDW g/L, of which 68.6% were PHBV with 3HV fraction of 26.0 mol% in the biopolymer, indicating promising potentials of commercialization in the future. (C) 2014 Elsevier B.V. All rights reserved. |
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