Vibrio salilacus sp. nov., a new member of Anguillarum clade with six alleles of the 16S rRNA gene from a saline lake----中国科学院微生物研究所

科研成果

论文题目:	Vibrio salilacus sp. nov., a new member of Anguillarum clade with six alleles of the 16S rRNA gene from a saline lake
作者:	Zhong Zhi-Ping, Liu Ying, Liu Hong-Can, Wang Fang, Zhou Yu-Guang, and Liu Zhi-Pei*.
联系作者:
刊物名称:	Int J Syst Evol Microbiol
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年份:	2015
影响因子:	2.632
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摘要:	A Gram-staining-negative, catalase- and oxidase-positive, facultative-aerobic bacterium, strain DSG-S6T, was isolated from Dasugan Lake (salinity 3.1 %, w/w). Its taxonomic position was determined by using a polyphasic approach. Cells of strain DSG-S6T were non-spore-forming, slightly bent rods, and motile by means of a single polar flagellum. Growth occurred in the presence of 0-7.0 % (w/v) NaCl (optimum, 2.0 %), at 4-35 degrees C (optimum, 30 degrees C) and pH 6.0-10.5 (optimum, pH 8.0-8.5). C16:0, C18:1omega7c and C16:1omega7c and/or C16:1omega6c were its major fatty acids. Six alleles of the 16S rRNA gene sharing 98.9-99.9 % similarities were detected in strain DSG-S6T, which showed highest 16S rRNA gene sequence similarity to Vibrio aestuarianus ATCC 35048T (97.7 %), then to V. pacinii LMG 19999T (97.6 %) and V. metschnikovii CIP 69.14T (96.8 %). Multilocus sequence analysis (MLSA) of four housekeeping genes and 16S rRNA genes clearly clustered it as a member of Anguillarum clade. DNA-DNA relatedness of strain DSG-S6T to V. aestuarianus NBRC 15629T, V. pacinii CGMCC 1.12557T and V. metschnikovii JCM 21189T was 20.6 +/- 2.3 %, 38.1 +/- 3.5 % and 24.2 +/- 2.8 %, respectively. The DNA G+C content was 46.8 mol % (Tm). Based on the data, it is concluded that strain DSG-S6T represents a novel species of the genus Vibrio, for which the name Vibrio salilacus sp. nov. is proposed, with DSG-S6T(=CGMCC 1.12427T= JCM 19265T) as the type strain.