Frigidibacter albus gen. nov., sp. nov., a novel genus of the family Rhodobacteraceae, isolated from lake water----中国科学院微生物研究所

科研成果

论文题目:	Frigidibacter albus gen. nov., sp. nov., a novel genus of the family Rhodobacteraceae, isolated from lake water
作者:	Li Aihua Zhou Yu-Guang*.
联系作者:
刊物名称:	Int J Syst Evol Microbiol
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年份:	2015
影响因子:	2.632
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摘要:	Three Gram-staining-negative, strictly aerobic, non-pigmented, non-motile, rod-shaped bacterial strains SP32T, SR68 and SP95 were isolated from two water samples of a coldwater lake in Xinjiang province, China. Growth was observed at 4-25 and pH6.0-9.0, optimum growth occurred at 18-20 and at pH7.0-7.5. Phylogenetic analysis of 16S rRNA gene sequences revealed that these isolates belonged to the family Rhodobacteraceae, but formed a distinct evolutionary lineage from other validly published species of this family. Strain SP32T showed the highest 16S rRNA gene sequences similarity (96.7%) to Rhodobacter veldkampii ATCC35703T, and the similarities to genera Defluviimonas, Haematobacter and Pseudorhodobacter were 95.8-96.4%, 96.0-96.1% and 95.3-96.1%, respectively. The genomic DNA G+C content of strain SP32T was 67.6 mol%. The major fatty acids (>5%) were summed feature 8 (C18:1omega7c/C18:1omega6c) and C18:1omega7c 11-methyl. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, one unidentified glycolipid and one unidentified polar lipid were found to be main polar lipids. Ubiquinone-10 (Q-10) was the sole respiratory quinone. While, strain SP32T neither produced photosynthetic pigments nor contained the gene pufM, by which it differed from the phototrophic species of the family Rhodobacteraceae. Based on the distinct phenotypic, chemotaxonomic and phylogenetic properties, strain SP32T represent a novel species in a novel genus within the family Rhodobacteraceae, for which we proposed the name Frigidibacter albus gen. nov., sp. nov. The type strain of the type species is strain SP32T (=CGMCC 1.13995T = NBRC 109671T ).