Colwellia aquaemaris sp. nov., isolated from the Cynoglossus semilaevis culture tank in a recirculating mariculture system----中国科学院微生物研究所

科研成果

论文题目:	Colwellia aquaemaris sp. nov., isolated from the Cynoglossus semilaevis culture tank in a recirculating mariculture system
作者:	Liu Ying, Liu Liang-Zi, Zhong Zhi-Ping, Zhou Yu-Guang, Liu Zhi-Pei*
联系作者:	Liu Zhi-Pei*
刊物名称:	Int J Syst Evol Microbiol
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年份:	2014
影响因子:	2.632
论文下载:	http://ijs.sgmjournals.org/content/early/2014/09/05/ijs.0.063305-0.long
摘要:	A Gram-staining-negative, heterotrophic, facultatively anaerobic bacterium, designated S1T, was isolated from the Cynoglossus semilaevis culture pond in a recirculating mariculture system in Tianjin, China. The taxonomy of strain S1T was studied by using a polyphasic approach. Cells of strain S1T were non-spore-forming, curved rods with 0.4-0.6 mum wide and 1.2-2.0 mum long, motile by means of a single polar fl agellum. It was positive for oxidase and catalase activities. Strain S1T was able to grow at 4-30 oC (optimum, 25 oC), at pH 5.5-10.0 (optimum, pH 6.5-7.5) and in the presence of 1-5 % (w/v) NaCl (optimum, 2 %). Strain S1T contained Q-8 as the sole respiratory quinone and C16:1omega7c/C16:1omega6c and C16:0 as the predominant cellular fatty acids. The genome DNA G+C content was 40.1 mol % (Tm). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S1T was placed in the genus Colwellia, and formed a distinct lineage in the phylogenetic tree together with Colwellia meonggei MA1-3T, C. aestuarii SMK-10T, C. polaris 537T and C. chukchiensis BCw111T, with 97.7 %, 96.1 %, 95.9 % and 95.0 % 16S rRNA gene sequence similarity, respectively. DNA-DNA relatedness of strain S1T to C. meonggei MA1-3T was 23.5 +/- 3.6 %. Based on the phylogenetic and phenotypic evidences, strain S1T is considered to represent a novel species of the genus Colwellia, for which the name Colwellia aquaemaris sp. nov. is proposed. The type strain is S1T (=CGMCC 1.12165T=JCM 18479T).