| 论文题目: | Resolution of carbon metabolism and sulfur-oxidation pathways of Metallosphaera cuprina Ar-4 via comparative proteomics |
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| 作者: | Jiang Cheng-Ying, Liu Li-Jun, Guo Xu, You Xiao-Yan, Liu Shuang-Jiang*, Poetsch Ansgar* |
| 联系作者: | Liu Shuang-Jiang*, Poetsch Ansgar* |
| 刊物名称: | J Proteomics |
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| 年份: | 2014 |
| 影响因子: | 4.12 |
| 论文下载: | http://www.sciencedirect.com/science/article/pii/S1874391914003492 |
| 摘要: | Metallosphaera cuprina is able to grow either heterotrophically on organics or autotrophically on CO2 with reduced sulfur compounds as electron donor. These traits endowed the species desirable for application in biomining. In order to obtain a global overview of physiological adaptations on the proteome level, proteomes of cytoplasmic and membrane fractions from cells grown autotrophically on CO2 plus sulfur or heterotrophically on yeast extract were compared. 169 proteins were found to change their abundance depending on growth condition. The proteins with increased abundance under autotrophic growth displayed candidate enzymes/proteins of M. cuprina for fixing CO2 through the previously identified 3-hydroxypropionate/4-hydroxybutyrate cycle and for oxidizing elemental sulfur as energy source. The main enzymes/proteins involved in semi- and non-phosphorylating Entner-Doudoroff (ED) pathway and TCA cycle were less abundant under autotrophic growth. Also some transporter proteins and proteins of amino acid metabolism changed their abundances, suggesting pivotal roles for growth under the respective conditions. BIOLOGICAL SIGNIFICANCE: The described work is of great significance: For general microbiology: For applied microbiology: |
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