| 论文题目: | Methanospirillum psychrodurum sp nova, isolated from wetland soil |
|---|---|
| 作者: | Zhou Liguang, Liu Xiaoli, Dong Xiuzhu* |
| 联系作者: | Dong Xiuzhu* |
| 刊物名称: | International Journal of Systematic and Evolutionary Microbiology |
| 期: | 64 |
| 卷: | |
| 页: | 638-641 |
| 年份: | 2014 |
| 影响因子: | 2.632 |
| 论文下载: | http://ijs.sgmjournals.org/content/64/Pt_2/638 |
| 摘要: | A psychrotolerant nnethanogenic strain, X-18(T), was isolated from the soil of the Madoi wetland at Qinghai, Tibetan plateau, China. Cells were wavy rods (11-62 mu m long) with blunt tapered ends and Gram-stain-negative. Strain X-18(T) grew strictly anaerobically and produced methane exclusively from H-2/CO2. Growth occurred in the temperature range of 4-32 degrees C and optimally at 25 degrees C. Growth pH ranged from 6.5 to 8.0 and the optimum was 7.0. The G C content of the genomic DNA of strain X-18(T) was 44.4 mol%. Phylogenetic analysis based on 165 rRNA gene sequences and the alpha subunit of methyl-coenzyme M reductase indicated that strain X-18(T) was affiliated to the genus Methanospirillum and was most closely related to Methanospirillum lacunae Ki8-(1T), with 96.3% 16S rRNA gene sequence similarity. However, strain X-18(T) could be distinguished from the existing species of the genus Methanospirillum by its lower growth temperature and obligate hydrogenotrophic methanogenesis. On the basis of phenotypic characteristics and phylogenetic analysis, strain X-18(T) represents a novel species of the genus Methanospirillum, for which the name Methanospirillum psychrodurum sp. nov. is proposed and strain X-18(T) is assigned as the type strain (=CGMCC 1.5186(T)=JCM 19216(T)). |
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