| 论文题目: | Flavobacterium lacus sp nov., isolated from a high-altitude lake, and emended description of Flavobacterium filum |
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| 作者: | Li Aihua, Liu Hongcan, Sun Bingda, Zhou Yuguang*, Xin Yuhua* |
| 联系作者: | Zhou Yuguang*, Xin Yuhua* |
| 刊物名称: | International Journal of Systematic and Evolutionary Microbiology |
| 期: | 64 |
| 卷: | |
| 页: | 933-939 |
| 年份: | 2014 |
| 影响因子: | 2.632 |
| 论文下载: | http://ijs.sgmjournals.org/content/64/Pt_3/933 |
| 摘要: | Two Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated NP180(T) and NR80, were isolated from water of Nam Co Lake, located in Tibet, China. Growth of strains NP180(T) and NR80 occurred at 4-25 degrees C and at pH 6.5-10.0 (optima, 15-20 degrees C and pH 7.5-8.5). The 16S rRNA gene sequence similarity to the phylogenetically closest related strains, Flavobacterium filum EMB 34(T), F. ponti GSW-R14(T) and F. gelidilacus LMG 21477(T), was 95.1, 94.8 and 94.6%, respectively. The predominant fatty acids were iso-C-15:0, iso-C-15:1 G, iso-C-17:0 3-OH and summed feature 9 (comprising iso-C-17:1 omega 9c and/or 10-methyl C-16:0). The major menaquinone of the two strains was menaquinone 6 (MK-6). Phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid were the major polar lipids in both strains. The G-C contents of the genomic DNA were 34.9 and 35.1 mol%, respectively, for strains NP180(T) and NR80. DNA-DNA relatedness between strains NP180(T) and NR80 was 99%, indicating that they belong to the same species. According to phylogenetic inference and phenotypic characteristics, a novel species, Flavobacterium lacus sp. nov., is proposed. The type strain is NP180(T) (=CGMCC 1.12504(T)=NBRC 109715(T)). An emended description of Flavobacterium Rum is also provided. |
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