| 论文题目: | Identification of hepatitis B virus-specific CTL epitopes presented by HLA-A*33:03 in peripheral blood mononuclear cells from patients and transgenic mice |
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| 作者: | Pan Xuwen, Ding Huiguo, Zhou Xuyu, Tien Po* |
| 联系作者: | Tien Po* |
| 刊物名称: | Biochemical and Biophysical Research Communications |
| 期: | 449 |
| 卷: | 1 |
| 页: | 135-140 |
| 年份: | 2014 |
| 影响因子: | 2.474 |
| 论文下载: | http://www.sciencedirect.com/science/article/pii/S0006291X14008456 |
| 摘要: | Cytotoxic T lymphocyte (CTL) epitopes in the HBV protein of hepatitis B virus (HBV) may play a key role in viral control and liver damage. The aim of this study was to identify and study the function of HLA-A*33:03-restricted CTL epitopes in HBV protein of the HBV genotypes B and C, which are epidemic in China. Sixteen HBV peptides were predicated by computational analysis, and synthesized peptides were examined for their affinity to HLA-A*33:03 using a stable cell line. After being analyzed by enzyme-linked immunospot and cytolytic activity assays, as well as the tetramers staining method using peripheral blood mononuclear cells isolated from HBV-infected patients, five peptides (Hbs245-253, HBs335-343, HBc119-127, HBc104-112, and HBp391-399) were chosen to further confirm their HLA_A*33:03 restriction in transgenic mice. (C) 2014 Elsevier Inc. All rights reserved. |
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