Characterization of human alphabetaTCR repertoire and discovery of D-D fusion in TCRbeta chains----中国科学院微生物研究所

科研成果

论文题目:	Characterization of human alphabetaTCR repertoire and discovery of D-D fusion in TCRbeta chains
作者:	Liu Peipei, Liu Di, Yang Xi, Gao Jing, Chen Yan, Xiao Xue, Liu Fei, Zou Jing, Wu Jun, Ma Juncai, Zhao Fangqing, Zhou Xuyu, George F Gao, Zhu Baoli
联系作者:	Zhou Xuyu, George F Gao, Zhu Baoli*
刊物名称:	Protein Cell
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年份:	2014
影响因子:	3.22
论文下载:	http://link.springer.com/article/10.1007%2Fs13238-014-0060-1
摘要:	The characterization of the human T-cell receptor (TCR) repertoire has made remarkable progress, with most of the work focusing on the TCRbeta chains. Here, we analyzed the diversity and complexity of both the TCRalpha and TCRbeta repertoires of three healthy donors. We found that the diversity of the TCRalpha repertoire is higher than that of the TCRbeta repertoire, whereas the usages of the V and J genes tended to be preferential with similar TRAV and TRAJ patterns in all three donors. The V-J pairings, like the V and J gene usages, were slightly preferential. We also found that the TRDV1 gene rearranges with the majority of TRAJ genes, suggesting that TRDV1 is a shared TRAV/DV gene (TRAV42/DV1). Moreover, we uncovered the presence of tandem TRBD (TRB D gene) usage in ~2% of the productive human TCRbeta CDR3 sequences.