Roseibium aquae sp. nov., isolated from a saline lake in Qinghai province, China----中国科学院微生物研究所

科研成果

论文题目:	Roseibium aquae sp. nov., isolated from a saline lake in Qinghai province, China
作者:	Zhong Zhi-Ping, Liu Ying, Liu Hong-Can, Wang Fang, Zhou Yu-Guang, Liu Zhi-Pei*
联系作者:	Liu Zhi-Pei*
刊物名称:	Int J Syst Evol Microbiol
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卷:
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年份:	2014
影响因子:	2.347
论文下载:	http://ijs.sgmjournals.org/content/early/2014/05/26/ijs.0.065508-0.long
摘要:	A Gram-negative bacterium, strain DSG-S4-2T, was isolated from Dasugan Lake, a saline lake (salinity 3.1 %, w/v) in Qaidam basin, Qinghai, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain DSG-S4-2T were non-spore-forming rods, 0.5-0.8 mum wide and 1.2-3.8 mum long, and motile by means of a single polar flagellum. Strain DSG-S4-2T was strictly heterotrophic and aerobic, catalase-positive and oxidase-negative. PufLM and coxL genes were present, bacteriochlorophyll a (BChl a) and a carotenoid pigment were produced. Growth was observed in the presence of 0-8.0 % (w/v) NaCl (optimum, 1.0-2.0 %), at 20-40 degrees C (optimum, 35 degrees C) and pH 6.5-10.5 (optimum, pH 7.5-8.0). Strain DSG-S4-2T contained Q-10 as the sole respiratory quinone. The polar lipids comprised of two aminolipids, diphosphatidglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, sulphoquinovosyldiacylglyceride, phosphatidylcholine and some unknown phospholipids, just as the other members of the genus Roseibium. The predominant fatty acid (>70 %) was summed feature 8 (C18:1 omega7c and/or C18:1 omega6c). The DNA G+C content was 61.4 mol % (Tm). Phylogenetic trees (NJ, ML and MP) based on 16S rRNA gene sequences showed that strain DSG-S4-2T was associated with the members of the genus Roseibium, with highest 16S rRNA gene sequence similarity to Roseibium denhamense OCh 254T (96.3 %) and Roseibium hamelinense OCh 368T (96.3 %). Based on the data presented above, it is concluded that strain DSG-S4-2T represents a novel species of the genus Roseibium, for which the name Roseibium aquae sp. nov. is proposed. The type strain is DSG-S4-2T (=CGMCC 1.12426T= JCM 19310T).