论文题目: | Cloning and analysis of the Oswc-1 gene encoding a putative blue light photoreceptor from Ophiocordyceps sinensis |
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作者: | Yang Tao, Xiong Weiping, Dong Caihong* |
联系作者: | Dong Caihong* |
刊物名称: | Mycoscience |
期: | 55 |
卷: | 4 |
页: | 241-245 |
年份: | 2014 |
影响因子: | 0.962 |
论文下载: | http://www.sciencedirect.com/science/article/pii/S1340354013001666 |
摘要: | Light plays an important role during both sexual and asexual growth of Ophiocordyceps sinensis, one of the best-known traditional Chinese medicines. In the present study, we cloned Oswc-1, the homologue of the blue light photoreceptor Ncwc-1 of Neurospora crassa, from O. sinensis by Hi-tail polymerase chain reaction. The deduced amino acid sequence of Oswc-1 contains the similar function domains as NcWC-1 including transcriptional activation, LOV (Light, Oxygen, or Voltage), PAS (Per-Arnt-Sim) and Zinc Finger domains. Phylogenetic analysis based on fungal WC-1 supported OsWC-1 was a blue light receptor. The expression of Oswc-1 messenger RNA was up-regulated upon irradiation significantly. (C) 2013 The Mycological Society of Japan. Published by Elsevier B. V. All rights reserved. |
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