The Aspergillus fumigatus beta-1,3-glucanosyltransferase Gel7 plays a compensatory role in maintaining cell wall integrity under stress conditions----中国科学院微生物研究所

科研成果

论文题目:	The Aspergillus fumigatus beta-1,3-glucanosyltransferase Gel7 plays a compensatory role in maintaining cell wall integrity under stress conditions
作者:	Zhao Wan*, Li Chunli, Liang Jinfgnan, Sun Shufeng
联系作者:	Zhao Wan*
刊物名称:	Glycobiology
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年份:	2014
影响因子:	3.962
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摘要:	Aspergillus fumigatus is an opportunistic fungal pathogen that causes fatal invasive aspergillosis (IA) among immunocompromised patients. The cell wall beta-1,3-glucan is mainly elongated by beta-1,3-glucanosyltransferase Gel family, which is vital for growth and virulence of A. fumigatus. Although seven members of Gels have been annotated, only Gel1, Gel2, and Gel4 were characterized. In this study, the function of Gel7 was analyzed for the first time, by construction the Deltagel7, Deltagel7Deltacwh41 and Deltagel1Deltagel7Deltacwh41 separately. Disruption of gel7 alone did not result in any obvious phenotype except an abnormality in conidia formation, whereas Deltagel7Deltacwh41 and Deltagel1Deltagel7Deltacwh41 exhibited abnormal conidiogenesis, a heat-induced delay of germination and a severe decrease in beta-1,3-glucan content. Our results suggested that the A. fumigatus beta-1, 3-glucanosyltransferase Gel7 was involved in conidiation, and was compensated for the cell wall beta-1,3-glucan defects when Gel1 and Gel2 lost their functions, especially at an elevated temperature.