miR444a has multiple functions in rice nitrate-signaling pathway----中国科学院微生物研究所

科研成果

论文题目:	miR444a has multiple functions in rice nitrate-signaling pathway
作者:	Yan Yongsheng, Wang Huacai, Hamera Sadia, Chen Xiaoying, Fang Rongxiang
联系作者:	Chen Xiaoying, Fang Rongxiang
刊物名称:	Plant J
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年份:	2014
影响因子:	7.113
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摘要:	Nitrate (NO3 - ) is a key signal molecule in plant metabolism and development, besides its role as a nutrient. It is known that Arabidopsis ANR1 is a major component in the NO3 - -signaling pathway triggering lateral root growth and miR444 which is specific to monocots targets four genes homologous to ANR1 in rice. Here, we show that miR444a plays multiple roles in the rice NO3 - -signaling pathway not only in root development, but also involving in nitrate accumulation and even in Pi starvation responses. miR444a overexpression resulted in reduced rice lateral root elongation, but promotion of rice primary and adventitious root growth, in a nitrate-dependent manner. In addition, overexpression of miR444a improved nitrate accumulation and expression of nitrate transporter genes under high nitrate concentration condition, but reduced the remobilization of nitrate from old leaves to young leaves thus affecting the plant adaptability to nitrogen limitation. Intriguingly, we found that Pi starvation strongly induced miR444 accumulation in rice roots and overexpression of miR444a altered Pi starvation-induced root architecture and enhanced Pi accumulation and expression of three Pi transporter genes. We further provided evidence that miR444a was involved in the interaction between the NO3 - - and Pi -signaling pathways in rice. Taken together, our observations demonstrate that miR444a plays multiple roles in the rice NO3 - -signaling pathway in nitrate-dependent root growth, nitrate accumulation and phosphate starvation responses. This article is protected by copyright. All rights reserved.