| 论文题目: | N-acetylglucosamine-induced white-to-opaque switching in Candida albicans is independent of the Wor2 transcription factor |
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| 作者: | Tong Yaojun, Cao Chengjun, Xie Jing, Ni Jian, Guan Guobo, Tao Li, Zhang Lixin*, Huang Guanghua* |
| 联系作者: | Zhang Lixin*, Huang Guanghua* |
| 刊物名称: | Fungal Genetics and Biology |
| 期: | 62 |
| 卷: | |
| 页: | 71-77 |
| 年份: | 2014 |
| 影响因子: | 3.379 |
| 论文下载: | |
| 摘要: | Candida albicans, a major opportunistic fungal pathogen of humans, can spontaneously undergo white-to-opaque switching, a prerequisite of mating. The phenotypes of white and opaque cells are heritable and bistable. The zinc-finger transcription factor Wor2 (White Opaque Regulator 2) has previously been identified as an important regulator of white-to-opaque switching. Deletion of WOR2 locks cells in the white phase when cultured on media containing glucose as the sole carbon source. In this study, we report that N-acetylglucosamine (GlcNAc) can induce white-to-opaque switching in the wor2/wor2 null mutant and stabilize the opaque phenotype of C. albicans. Moreover, overexpression of RAS1V13 (the activating form of RAS1) hypersensitizes white cells of the wor2/wor2 mutant to GlcNAc. These results suggest that Wor2 is not required for opaque cell formation at least under some culture conditions. Therefore C. albicans cells may adopt a different gene expression profile in response to GlcNAc to activate phenotypic switching. (C) 2013 Elsevier Inc. All rights reserved. |
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